Identification of major proteins in sclerotia of Sclerotinia minor and Sclerotinia trifoliorum

Petersen, Gary R.; Russo, Geraldine M.; Etten, James L. Van

doi:10.1016/0147-5975(82)90119-0

Cited by 21 publications

(16 citation statements)

References 8 publications

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“…These markers include proteins (Petersen et al 1982;Tariq et al 1985), random amplified polymorphic DNA (RAPD) (Ekins 2000) and restriction fragment length polymorphisms (RFLP) (Kohn et al 1988;Ekins et al 2005). Recently Powers et al (2001) reported an intron in the nuclear ribosomal DNA of S. trifoliorum species that distinguishes it from S. sclerotiorum and S. minor.…”

Section: Introductionmentioning

confidence: 97%

“…S. minor can be relatively easily differentiated from the other two species based on the small size of the sclerotia and their abundant formation on host tissues (Petersen et al 1982). Growth rate and sclerotial characteristics have also been used to separate S. sclerotiorum from S. trifoliorum (Petersen et al 1982;Tariq et al 1985;Willetts and Wong, 1980). Morphological differentiation of S. sclerotiorum and S. trifoliorum relies on the size of ascspores within the ascus.…”

Section: Introductionmentioning

confidence: 99%

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Occurence of sclerotinia stem rot of fenugreek caused by Sclerotinia trifoliorum and S. sclerotiorum in Tunisia

Gargouri¹,

Berraies

Gharbi³

et al. 2017

Eur J Plant Pathol

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Fenugreek is an annual leguminous crop grown for hay and grains in Tunisia. It is also considered a valuable rotation crop with cereals. Sclerotinia rot was observed in production fields since 2010. The survey conducted in 2013 revealed that the incidence of diseased plants varied between 5 and 20%. The identification of isolates of Sclerotinia obtained from fenugreek plants with symptoms of stem rot was determined using morphological and molecular criteria. The size, shape and abundance of sclerotia in potato dextrose agar (PDA) cultures were used to classify isolates as S. sclerotiorum or S. trifoliorum. A comparison of colony diameter on PDA after 24, 48 and 72 h at 25°C, showed that one isolate grew faster (36 mm/ day) than the other 10 isolates (14.8 mm/day). There was a significant difference in sclerotial size between the fast and the slow growing isolates, but there was no significant difference in the number of sclerotia produced after 3 weeks on PDA. Two of the slow growing isolates exhibited ascospore dimorphism, whereas the fast growing isolate did not. PCR amplification with the primer pair ITS5/ITS4 produced a fragment of 560 base pairs from the fast growing isolate and 1000 base pairs from all of the slow growing isolates. The ITS sequences of the fast growing isolate had 100% homology with S. sclerotiorum, whereas those of the slow growing isolates had 100% homology with S. trifoliorum. Isolates of both species were pathogenic on fenugreek seedlings in the greenhouse assay and there was no significant difference in the percentage of dead plants two weeks after inoculation between the two species.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Occurence of sclerotinia stem rot of fenugreek caused by Sclerotinia trifoliorum and S. sclerotiorum in Tunisia

Gargouri¹,

Berraies

Gharbi³

et al. 2017

Eur J Plant Pathol

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“…This protein is found not only in S. sclerotiorum, but also in other Sclerotinia spp. (Petersen et al, 1982). Immunoblots and ELISA analysis using polyclonal anti-SSP antibodies further revealed that related proteins are present universally in determinate sclerotia-forming species of the Sclerotiniaceae but cross-reacting proteins were not present in substratal stroma-forming species or sclerotial forming species outside of the Sclerotiniaceae (Novak and Kohn, 1991).…”

Section: Introductionmentioning

confidence: 97%

The development-specific ssp1 and ssp2 genes of Sclerotinia sclerotiorum encode lectins with distinct yet compensatory regulation

Li¹,

Rollins²

2010

Fungal Genetics and Biology

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“…Until recently sclerotia were thought to contain mostly carbohydrates and lipids as storage compounds (Chet et al, 1969;Bullock et al, 1980). However, since large amounts of proteins have been found in sclerotia of Sclerotinia minor and Sclerotinia trifoliorum (Petersen et al, 1982), and of Sclerotium rolfsii and Botrytis cinerea (Insell et al, 1985) their role as storage proteins in these resting structures becomes apparent.…”

Section: Y Imentioning

confidence: 99%

Developmental accumulation of lectin in Rhizoctonia solani: a potential role as a storage protein

Kellens

Peumans

1990

Journal of General Microbiology

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The lectin in Rhizoctonia solani is a major protein, although its function was previously unknown. An enzymelinked immunosorbent assay was developed to quantify the lectin content in mycelium, sclerotia and culture filtrate during the development of R. solmi grown in a synthetic medium. Lectin content was low in young mycelium but increased dramatically at the onset of sclerotium formation, reaching a maximum in adult sclerotia. Upon myceliogenic germination, the lectin content of the sclerotia rapidly decreased. It thus appears that the lectin is a developmentally regulated sclerotium-specific protein accumulating during sclerotium formation and disappearing during germination. Its relative abundance and the pattern of developmental control suggests that the lectin is probably a storage protein. Lectin accumulation was also influenced by the composition of the medium. Addition of supplementary carbohydrate (D-glucose) caused a marked reduction in lectin content whereas increased nitrogen in the form of L-asparagine led to a higher lectin content. However, when the L-asparagine concentration was too high, autolysis occurred and part of the lectin was released into the medium.

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Identification of major proteins in sclerotia of Sclerotinia minor and Sclerotinia trifoliorum

Cited by 21 publications

References 8 publications

Occurence of sclerotinia stem rot of fenugreek caused by Sclerotinia trifoliorum and S. sclerotiorum in Tunisia

Occurence of sclerotinia stem rot of fenugreek caused by Sclerotinia trifoliorum and S. sclerotiorum in Tunisia

The development-specific ssp1 and ssp2 genes of Sclerotinia sclerotiorum encode lectins with distinct yet compensatory regulation

Developmental accumulation of lectin in Rhizoctonia solani: a potential role as a storage protein

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