Identification of major phenolic compounds of Aloe vera and its protective effect on ovaries under oxidative stress in diabetic rats

Abstract

The study investigated the major phenolic compounds of Aloe vera leaf (AVL) by the high-performance liquid chromatography (HPLC) method, and its protective effect on ovaries under oxidative stress of streptozotocininduced diabetic rats. The study was conducted on control (untreated healthy rats; C), diabetes (untreated diabetic rats; D) and diabetes+A. vera treatment (diabetic rats treated with A. vera; D+A) groups. D+A group was given an ethanolic extract of A. vera (300 mg/kg) for 14 days. Major phenolic compounds of AVL were chlorogenic acid and rutin. alanine transaminase (ALT) and aspartate aminotransferase (AST) levels increased in D group compared to others groups (P<0.01). Alkaline phosphatase (ALP) and albumin levels were lower and higher in the C group than in other groups, respectively (P<0.01). In the D group compared to other groups, oxidative stress index (OSI) and total oxidant status levels increased, while total antioxidant status level decreased in both ovarian tissues and blood (P<0.01). Preantral and antral follicle counts decreased in the D group according to other groups (P<0.01) and atretic follicle count increased (P<0.05). Proliferating cell nuclear antigen (PCNA) expression level (P<0.01), and B-cell lymphoma-2-associated-Xprotein (Bax; P<0.01) and tumor necrosis factor-alpha (TNF-α) expression levels (P<0.05) decreased and increased in group D according to other groups, respectively. In conclusion, treatment with AVL decreased OSI, improved follicular dynamics, and restored Bax, TNF-α and PCNA expression in diabetic rats.

Protective effect of Gundelia tournefortii extract on spleen tissue in experimental type I diabetes in rats

“…Diabetes mellitus, which is an important health problem for humans, has been studied many times, and it is still one of the most frequently studied subjects in the scientific world [19,20,24]. In many of these studies, histopathologic examination was performed, and it was revealed that diabetes has a splenotoxic effect.…”

“…Animals in Diabetes + Treatment 1, Diabetes + Treatment 2, and Diabetes + Treatment 3 groups were administered 50 mg/kg, 100 mg/kg, and 200 mg/kg of Gundelia tournefortii extract daily by oral gavage for 21 days, respectively. At the end of the experiment, animals were sacrificed under general anesthesia (Xylazine 10 mg/kg and Ketamine 5 mg/kg), and spleen tissues were removed under appropriate storage conditions [19].…”

“…Gundelia tournefortii has been reported to trigger the translocation of Glucose transporter type 4 (GLUT4) protein to the plasma membrane in muscle cells, and there are inferences that alcoholic and hexane extracts of the plant can be used as anti-diabetic [15]. In the literature reviews, there are studies investigating the protective effects of experimental type I diabetes on the spleen and other tissues [16][17][18][19][20][21]. This study aimed to histopathologically investigate the effects of Gundelia tournefortii plant extract on the spleen in rats with experimental type.…”

A novel electrochemical sensor based on a molecularly imprinted polymer for highly selective and sensitive determination of rutin from herbal supplements and plant extracts

“…Phenolic compounds show strong antioxidant effects in general. [1][2][3][4] Rutin is also called vitamin P in some sources and stands out by exhibiting a variety of pharmacological actions, including anti-inammatory, anti-allergic, antioxidant, antidiabetic, antihypertensive, cardioprotective, and neuroprotective effects. 5,6 RUT is included in many plants that people frequently consume daily, such as coffee, rooibos, buckwheat, etc.…”