Identification of  LsrB regulon

An, Fang; Li, Ningning; Zhang, Lanya; Zheng, Wei; Xing, Shenghui; Tang, Gong‐Jian; Yan, Jun; Yu, Liangliang; Luo, Li

doi:10.1093/abbs/gmab054

Cited by 2 publications

(2 citation statements)

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“…JspA increases expression of lsrB, which encodes a LysR-family transcription factor required for effective nodulation (Luo et al, 2005) and involved in the differential expression of over 200 genes, including many that regulate redox homeostasis (An et al, 2021). Deletion of lsrB resulted in poor growth and increased sensitivity to the detergent deoxycholate (Barnett et al, 2019), and lsrB orthologs in B. abortus and A. tumefaciens contribute to pathogenesis (Budnick et al, 2020;Eisfeld et al, 2021;Sheehan et al, 2015;Tang et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

A protease and a lipoprotein jointly modulate the conserved ExoR-ExoS-ChvI signaling pathway critical inSinorhizobium melilotifor symbiosis with legume hosts

Bustamante,

Ceron,

Gao

et al. 2023

Preprint

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Sinorhizobium melilotiis a model alpha-proteobacterium for investigating microbe-host interactions, in particular nitrogen-fixing rhizobium-legume symbioses. Successful infection requires complex coordination between compatible host and endosymbiont, including bacterial production of succinoglycan, also known as exopolysaccharide-I (EPS-I). InS. melilotiEPS-I production is controlled by the conserved ExoS-ChvI two-component system. Periplasmic ExoR associates with the ExoS histidine kinase and negatively regulates ChvI-dependent expression ofexogenes, necessary for EPS-I synthesis. We show that two extracytoplasmic proteins, LppA (a lipoprotein) and JspA (a metalloprotease), jointly influence EPS-I synthesis by modulating the ExoR-ExoS-ChvI pathway and expression of genes in the ChvI regulon. Deletions ofjspAandlppAled to lower EPS-I production and competitive disadvantage during host colonization, for bothS. melilotiwithMedicago sativaandS. medicaewithM. truncatula. Overexpression ofjspAreduced steady-state levels of ExoR, suggesting that the JspA protease participates in ExoR degradation. This reduction in ExoR levels is dependent on LppA and can be replicated with ExoR, JspA, and LppA expressed exogenously inCaulobacter crescentusandEscherichia coli. Akin to signaling pathways that sense extracytoplasmic stress in other bacteria, JspA and LppA may monitor periplasmic conditions during interaction with the plant host to adjust accordingly expression of genes that contribute to efficient symbiosis. The molecular mechanisms underlying host colonization in our model system may have parallels in related alpha-proteobacteria.Author summarySymbiotic bacteria that live in the roots of legume plants produce biologically accessible nitrogen compounds, offering a more sustainable and environmentally sound alternative to industrial fertilizers generated from fossil fuels. Understanding the multitude of factors that contribute to successful interaction between such bacteria and their plant hosts can help refine strategies for improving agricultural output. In addition, because disease-causing microbes share many genes with these beneficial bacteria, unraveling the cellular mechanisms that facilitate host invasion can reveal ways to prevent and treat infectious diseases. In this report we show that two genes in the model bacteriumSinorhizobium meliloticontribute to effective symbiosis by helping the cells adapt to living in host plants. This finding furthers knowledge about genetics factors that regulate interactions between microbes and their hosts.

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Section: Discussionmentioning

confidence: 99%

A protease and a lipoprotein jointly modulate the conserved ExoR-ExoS-ChvI signaling pathway critical inSinorhizobium melilotifor symbiosis with legume hosts

Bustamante,

Ceron,

Gao

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…For example, JspA inhibits expression of the transcription regulator LdtR, which plays a role in osmotic stress tolerance, motility, and likely cell wall remodeling [126,127]. JspA increases expression of lsrB, which encodes a LysR-family transcription factor required for effective nodulation [128] and involved in the differential expression of over 200 genes, including many that regulate redox homeostasis [129]. Deletion of lsrB resulted in poor growth and increased sensitivity to the detergent deoxycholate [127], and lsrB orthologs in B. abortus and A. tumefaciens contribute to pathogenesis [130][131][132][133].…”

Section: Plos Geneticsmentioning

confidence: 99%