Identification of loci associated with embryo yield in microspore culture of Brassica rapa by segregation distortion analysis

Abstract

We identified three physical positions associated with embryo yield in microspore culture of Brassica rapa by segregation distortion analysis. We also confirmed their genetic effects on the embryo yield. Isolated microspore culture is well utilized for the production of haploid or doubled-haploid plants in Brassica crops. Brassica rapa cv. 'Ho Mei' is one of the most excellent cultivars in embryo yield of microspore culture. To identify the loci associated with microspore embryogenesis, segregation analysis of 154 DNA markers anchored to B. rapa chromosomes (A01-A10) was performed using a population of microspore-derived embryos obtained from an F1 hybrid between 'CR-Seiga', a low yield cultivar in microspore-derived embryos, and 'Ho Mei'. Three regions showing significant segregation distortion with increasing 'Ho Mei' alleles were detected on A05, A08 and A09, although these regions showed the expected Mendelian segregation ratio in an F2 population. The additive effect of alleles in these regions on embryo yield was confirmed in a BC3F1 population. One region on A08 containing Br071-5c had a higher effect than the other regions. Polymorphism of nucleotide sequences around the Br071-5c locus was investigated to find the gene possibly responsible for efficient embryogenesis from microspores.

Identification and Characterization of Segregation Distortion Loci on Cotton Chromosome 18

“…With the development of molecular markers, SD has been widely reported in several plant species, particularly in interspecific crosses for genetic mapping, such as rice (Shanmugavadivel et al, 2013), wheat (Takumi et al, 2013; Adamski et al, 2014), chickpea (Ravikumar et al, 2013), Arabidopsis (Leppala et al, 2013), coffee (Gartner et al, 2013), soybean (Baumbach et al, 2012), potato (Manrique-Carpintero et al, 2016), Brassica rapa (Kitashiba et al, 2016), and Populus deltoids (Zhou et al, 2015). SD has previously been reported in interspecific populations of cotton (Guo et al, 2007; Blenda et al, 2012; Byers et al, 2012; Liang et al, 2012; Yu et al, 2013; Diouf et al, 2014; Zhang et al, 2014; Li et al, 2016), intraspecific populations of G. barbadense (Wang et al, 2013), intraspecific populations of G. hirsutum (Lin et al, 2009; Zhang et al, 2012, 2016; Ning et al, 2014), and intraspecific populations of G. arboreum (Li et al, 2007); however, the genetic mechanism of SD in cotton remains unclear.…”

Overview of In Vitro and In Vivo Doubled Haploid Technologies

“…Even within a species, there will be varieties, lines and even individuals that respond differently. This strong influence of the genotype, together with the fact that this trait is transmitted across generations and segregates in the hybrids offspring [9,10], indicates that it is under genetic control [11][12][13][14][15][16][17]. Furthermore, it was proposed that, at least for Brassica napus, the embryogenic competence of microspores is controlled by two loci with additive effects [10].…”

The Resistance of Oilseed Rape Microspore-Derived Embryos to Osmotic Stress Is Associated With the Accumulation of Energy Metabolism Proteins, Redox Homeostasis, Higher Abscisic Acid, and Cytokinin Contents

“…More than 340 and 78 article topics and titles, respectively, focused on "B. napus + microspore, " were published in the 2005-2020 period (Web of Science). Recently, Kitashiba et al (2016) have identified loci associated with embryo yield in microspore culture of Brassica rapa. Daurova et al changed microspore-derived embryos (MDE) fatty acid composition (Daurova et al, 2020).…”