Identification of leptospira serovars by restriction-endonuclease analysis

Marshall, R.B.; Wilton, B. E.; Robinson, Anthony J.

doi:10.1099/00222615-14-1-163

Cited by 154 publications

(83 citation statements)

References 13 publications

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“…The two isolates of serovar hardjo originated from same animal species and produced identical restriction patterns. These findings support previous reporteds about no detectable variation in the restriction endonuclease fingerprints among isolates from different species of animals or among isolates from the same host species (Marshall et al, 1981;Robinson et al, 1982, Bolin andZuerner, 1996;Djorjevic et al, 1993). Identical restriction patterns of isolates from different species was suggestive of the stability of the genome of the organism.…”

Section: Discussionsupporting

confidence: 82%

“…DNΑ-based techni ques were introduced to identify leptospiral serovars and even they couldn't differentiate between field strains (Zuerner and Bolin, 1990;Pacciarini et al, 1992;Djordjevic et al,1993;Zuerner et al, 1993;Corney and Colley, 1996;Bolin and Zuerner, 1996;Letocard et al, 1997;Rocha, 2004). Restriction endonuclease analysis (REA) has been widely used as an epidemiological tool and a typing method for bacterial isolates of public Marshall et al (1981) were the first to apply the REA in the classification of leptospires. Since then, after the improvement of DNA extraction methods and the resolution of the restriction fragments, the REA has been widely used to differen tiate and classify leptospires (Marshall et al, 1984;Thiermann et al, 1985Thiermann et al, , 1986Terpstra et al, 1987;Tamai et al, 1988;Ellis et al, 1988;Thiermann and Le Vebvre, 1988;Silbreck and Davis, 1989;Zuerner and Bolin., 1990;Woodward and Redstone, 1993;Corney and Colley, 1996;Bolin and Zurner, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Differentiation of leptospiral serovars by restriction endonucleases analysis

Eljalii

Ali

2017

J Hellenic Vet Med Soc

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ΠΕΡΙΛΗΨΗ.* DNA από δέκα στελέχη λεπτόσπειρας χρησιμο ποιήθηκε για πέψη με χα ένζυμα περιορισμού HindUl, BamHi και EcoEl, με σκοπό χη διαφοροποίηση χους ανά ορόχυπο. Μεταξύ χων ορόχυπων που εξεχάοχηκαν διαπιοχώθηκε υψηλή εχερογέ-νεια. Ομοιότητες αναδείχθηκαν μεχαξυ στελεχών χου ίδιου ορό-χυπου. Δεν διαπιστώθηκε συσχέχιση μεταξύ του είδους από το οποίο απομονώθηκαν τα στελέχη που εξετάστηκαν και των τύπων στους οποίους διακρίθηκαν βάσει τυποποίησης με ένζυμα περιο ρισμού. Φαίνεται ότι η ανάλυση με τη χρήση ένζυμων περιορισμού θα ήταν χρήσιμη στη διάκριση ορότυπων της λεπτόσπειρας. * Η απόδοση της περίληψης στα ελληνικά έγινε από τον κ. Οίκο-νομόπουλο Ιωάννη, Κτηνίατρο, ΓΠΑ.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Differentiation of leptospiral serovars by restriction endonucleases analysis

Eljalii

Ali

2017

J Hellenic Vet Med Soc

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“…The BRENDA technique has been described previously by Marshall, Wilton & Robinson (1981) and Kakoyiannis, Winter & Marshall (1984 24 unique patterns. The patterns from two of these were indistinguishable from the patterns given by a poultry isolate.…”

Section: Methodsmentioning

confidence: 99%

“…Bacterial restriction endonuclease DNA analysis (BRENDA) has already been shown to be a useful technique for the identification of a broad spectrum of different organisms, including Leptospira interrogans (Marshall, Wilton & Robinson, 1981) Vibrio cholerae (Kaper et al 1982), Rickettsia prowazekii (Regnenery et al 1983), Moraxella bovis (Marshall et al 1985) and Campylobacter coli (Kakoyiannis, Winter & Marshall, 1984).…”

Section: Introductionmentioning

confidence: 99%

The relationship between intestinalCampylobacterspecies isolated from animals and humans as determined by BRENDA

1988

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SUMMARYIntestinal thermophilic Campylobacter species produce stable patterns when subjected to bacterial restriction endonuclease DNA analysis (BRENDA); this technique is therefore of considerable value in epidemiological studies. BRENDA was used to examine thermophilic Campylobacter species from humans, wild and domestic animals. One hundred and ninety-four (61 %) of 316 isolates of Campylobacter jejuni from humans had BRENDA patterns which could be matched to those of animal isolates. Poultry appear to be a major source of infection for C. jejuni in humans with nearly half (49 7 %) of the human isolates giving patterns which were indistinguishable from those isolated from poultry. A total of 60 BRENDA types were identified from 316 human isolates and 11 of these had the same pattern as those isolated from poultry. One of the three Campylobacter coli BRENDA types recovered from poultry was indistinguishable from a human isolate type. Pigs appear to be only a minor source of C. coli infection for humans in New Zealand. Rats were found to be infected with strains of C. jejuni with BRENDA patterns indistinguishable from those infecting humans, poultry and a horse. None of the 102 isolates of Campylobacter species from wild birds gave BRENDA patterns similar to those of isolates from humans.

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“…riants in different types of taxa, including leptospires (13,17), Mycobacterium bovis (8), mycoplasmas (20,21), Rickettsia prowazekii (22), Campylobacter jejuni (7), and Neisseria meningitidis (3). Furthermore, REA has been used for identification of closely related species in the genera Bacteroides (6), Brucella (18), Mycobacterium (12), and Lactobacillus (16).…”

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confidence: 99%

Restriction Endonuclease Patterns and Multivariate Analysis as a Classification Tool for Lactobacillus spp.

Ståhl

Molin

Persson

et al. 1990

International Journal of Systematic Bacteriology

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Three Lactobacillus plantarum and seven Lactobacillus reuteri strains were studied by using restriction endonuclease analysis (REA) combined with principal-component analysis (PCA) and soft independent modeling of class analogy (SIMCA). Chromosomal DNAs from the strains were extracted and cleaved with restriction enzymes, and the DNA fragments were separated according to size by agarose gel electrophoresis. Band patterns were read by using a laser densitometer. The procedure to obtain reproducible digestion patterns with a suitable number of DNA fragments was optimized. Asp 718, ClaI and EcoRI were the most suitable of the 17 restriction enzymes tested, each giving 30 to 50 DNA fragments down to a molecular weight of 4.2 X lo6. The digestion patterns of all three enzymes, which gave a data set for 10 strains and 80 variables, were used for classification by PCA and SIMCA. All strains were clearly separated, and the separation within each species was in general accordance with data on DNA-DNA homology found in the literature. We concluded that restriction endonuclease analysis, combined with PCA and SIMCA, can be used for the classification of Lactobacillus spp.The genus Lactobacillus comprises about 50 validly described species; for historical reasons, in this genus circumscription of taxa has been based mainly on phenotypic features (e.g., the ability to ferment carbohydrates). Furthermore, the members of the genus Lactobacillus are formally differentiated from members of the genera Leuconostoc, Lactococcus, and Pediococcus on morphological grounds; in several cases this differentiation has caused systematic problems, as it can be diacult to distinguish between rods and cocci (23). Numerical studies have revealed the weakness in the phenotypic foundation (5,19). The systematics of this group have been improved by chemotaxonomy and DNA-DNA homology studies. Thus, the genus consists of deterministically defined taxa, but the relationships among the taxa are vague.Examples of characteristics that indicate the weakness in the present definition of the genus Lactobacillus are (i) the wide range in guanine-plus-cytosine contents of DNAs from different Lactobacillus species (32 to 53 mol%), (ii) the different kinds of cross-linkage of the peptidoglycans in cell walls, and (iii) the mingling in a phylogenetic tree of Lactobacillus, Leuconostoc, and Pediococcus strains as indicated by rRNA sequencing data (25). Thus, there seems to be a need to study the genus Lactobacillus with reclassification in mind. What method should be applied? Sequencing of rRNA is technically difficult and labor intensive. The accuracy of using enzymes as genetic markers (15) can be questioned as long as the majority of the information in the DNA remains unknown. And DNA-DNA homology is, unfortunately, not suitable outside the constraints of highly related species. An alternative method is restriction endonuclease analysis (REA), which, compared with rRNA sequencing, is technically easy to handle and may, in contrast to DNA-DNA homology, have g...

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Identification of leptospira serovars by restriction-endonuclease analysis

Cited by 154 publications

References 13 publications

Differentiation of leptospiral serovars by restriction endonucleases analysis

Differentiation of leptospiral serovars by restriction endonucleases analysis

The relationship between intestinalCampylobacterspecies isolated from animals and humans as determined by BRENDA

Restriction Endonuclease Patterns and Multivariate Analysis as a Classification Tool for Lactobacillus spp.

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