Identification of Leishmania species by kinetoplast DNA-polymerase chain reaction for the first time in Khaf district, Khorasan-e-Razavi province, Iran

Fata, Abdolmajid; Ghodratollah, Salehi Sangani; Hushang, Rafatpanah; Bazzaz, Mojtaba Mousavi; Mohaghegh, Mohammad Ali; Abdolghayoum, Movahedi

doi:10.4103/2229-5070.145587

Cited by 15 publications

(9 citation statements)

References 11 publications

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“…The groups were compared using one-way ANOVA test, followed by Tukey's honest significant difference (HSD) test at P value below 0.05. The Microsoft Excel software (Microsoft Inc.) was used to determine the lethal (1) Mortality rate :…”

Section: Discussionmentioning

confidence: 99%

“…At each interval (6,24,48, and 72 hours), 10 µL of each sample was gently mixed with 10 µL of 0.4% trypan blue on a glass slide. The mortality of parasites was determined by counting the number of alive (not stained) and dead (stained) promastigotes according to the following formula (1). Accordingly, at least 50 microscopic fields were examined for each sample.…”

Section: Experimentationmentioning

confidence: 99%

“…Cutaneous leishmaniasis (CL) is an important zoonotic disease worldwide (1,2). Currently, pentavalent antimonials, such as meglumine antimoniate (Glucantime ® ), are used as first-line treatment for CL (3).…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Antileishmanial Activity of Falcaria vulgaris Fractions on Leishmania major

AliEskandarian

HamedJafari

Asghari

et al. 2017

Jundishapur J Nat Pharm Prod

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Section: Discussionmentioning

confidence: 99%

Section: Experimentationmentioning

confidence: 99%

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In Vitro Antileishmanial Activity of Falcaria vulgaris Fractions on Leishmania major

AliEskandarian

HamedJafari

Asghari

et al. 2017

Jundishapur J Nat Pharm Prod

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“…[4161721] Therefore, it is an ideal target for the identification of Leishmania species. [22] Hence, we used kDNA-PCR to determine the genetic diversity of this protozoon, in Ilam Province.…”

Section: Introductionmentioning

confidence: 99%

Determination of genetic diversity of Leishmania species using mini-circle kDNA, in Iran-Iraq countries border

et al. 2018

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Background and Objective:Cutaneous leishmaniasis (CL) is one of the most important diseases worldwide, with a different range of prevalence in endemic areas. Anthroponotic and zoonotic CL are two epidemiological forms of CL, in Iran. Although Ilam Province in the west of Iran is one of the main endemic areas of the disease, there is no inclusive study to determine the genetic variations of parasite in these areas. The objective of this study was to determine the genetic diversity of Leishmania species in Ilam Province, using mini-circle kDNA gene.Materials and Methods:Direct smears were taken from skin lesions of 200 suspected cases of CL. Smears were stained, screened under light microscope. Polymerase chain reaction (PCR) was performed, using specific kinetoplast DNA primers. Data were analyzed, using the molecular bio-software.Results:All the samples were positive by direct examination. PCR results showed all cases were positive for Leishmania major. Although all isolated cases belong to a different county of Ilam province, all were positive for L. major with intra-species genetic diversity, divided into four clades in the dendrogram.Interpretation and Conclusion:This variation can affect drug resistance and controlling strategies of parasite. It is possible that different species of sand flies and rodents are the vector and reservoir of parasite, respectively; however, further studies are needed to validate this.

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“…It was reported that the 45 isolates of Leishmania from different geographical areas of Iran were suspected of cutaneous leishmaniasis (4). Every year it was reported that at least 20 000 cases of cutaneous leishmaniasis are in Iran (5).…”

Section: Introductionmentioning

confidence: 99%

Identification of Leishmania Species for Cutaneous leishmaniasis in Gonabad, Bardaskan and Kashmar, Central Khorasan, 2015

Rezai

Moghaddas

Bagherpor

et al. 2017

Jundishapur J Microbiol

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Background: About 90% of cutaneous leishmaniasis (CL) cases are reported from 7 countries including Iran. In this study, the cutaneous leishmaniasis species causing CL in Gonabad, Bardaskan, Kashmar cities in central Khorasan (Iran) were identified by kDNA-PCR. Methods:During the study, 93 suspected patients with CL, who were referred to the dermatology research center in these cities, were evaluated based on age, clinical forms, and place of residence. Direct microscopy was employed for parasitological diagnosis and PCR from skin ulcers performed using specific kDNA primers. Data were analyzed using the SPSS software.Results: Among 93 individuals with skin ulcers suspected to CL, the results of 81 direct smears were positive. PCR bands were observed in 84 examined samples, of which 68 (81%) samples were identified for Leishmania tropica and 16 (19%) samples for L. major. 12 patients were from Bardaskan with 10 cases of L. tropica, 23 patients from Gonabad with 20 cases of L. tropica, and 49 patients from Kashmar with 38 cases of L. tropica. Most of the lesions were located on hands (37%), the most clinical feature was papule (75%), and most of patients were 21 -30 years old. Conclusions:Previous epidemiologic studies have indicated that anthroponotic cutaneous leishmaniasis is the only dominant CL in the center and south of Khorasan. However, this study introduced new foci of zoonotic cutaneous leishmaniasis in these areas. Kashmar city was introduced as a new focus of zoonotic cutaneous leishmaniasis.

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Identification of Leishmania species by kinetoplast DNA-polymerase chain reaction for the first time in Khaf district, Khorasan-e-Razavi province, Iran

Cited by 15 publications

References 11 publications

In Vitro Antileishmanial Activity of Falcaria vulgaris Fractions on Leishmania major

In Vitro Antileishmanial Activity of Falcaria vulgaris Fractions on Leishmania major

Determination of genetic diversity of Leishmania species using mini-circle kDNA, in Iran-Iraq countries border

Identification of Leishmania Species for Cutaneous leishmaniasis in Gonabad, Bardaskan and Kashmar, Central Khorasan, 2015

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