Identification of Leishmania at the species level with matrix-assisted laser desorption ionization time-of-flight mass spectrometry

Cassagne, Christophe; Pratlong, Francine; Jeddi, Fakhri; Benikhlef, Razika; Karim, Abdul; Normand, Anne‐Cécile; Faraut, Françoise; Bastien, Patrick; Piarroux, Renaud

doi:10.1111/1469-0691.12387

Cited by 35 publications

(38 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recently, mass spectrometry was suggested for typing of Leishmania parasites (23,101), providing a promising means of rapid identification to the species level. Nevertheless, it is suitable only for identifying cultured parasites, and spectral data are not comparable across different laboratories.…”

Section: Other Culture-dependent Methodsmentioning

confidence: 99%

Species Typing in Dermal Leishmaniasis

2015

View full text Add to dashboard Cite

SUMMARY Leishmania is an infectious protozoan parasite related to African and American trypanosomes. All Leishmania species that are pathogenic to humans can cause dermal disease. When one is confronted with cutaneous leishmaniasis, identification of the causative species is relevant in both clinical and epidemiological studies, case management, and control. This review gives an overview of the currently existing and most used assays for species discrimination, with a critical appraisal of the limitations of each technique. The consensus taxonomy for the genus is outlined, including debatable species designations. Finally, a numerical literature analysis is presented that describes which methods are most used in various countries and regions in the world, and for which purposes.

show abstract

Section: Other Culture-dependent Methodsmentioning

confidence: 99%

Species Typing in Dermal Leishmaniasis

2015

View full text Add to dashboard Cite

show abstract

“…Forty-seven clinical isolates were harvested from patients diagnosed with cutaneous leishmaniasis (CL) (18 isolates) or visceral leishmaniasis (VL) (29 isolates) from the Maghreb (26 isolates) and southern France (21 isolates). Clinical isolates derived from the Maghreb were identified at the species level by either isoenzyme analysis performed at the Pasteur Institute of Algeria or matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry performed at the parasitology department of La Timone hospital in Marseilles, France (25,26). French isolates were identified at the species level by isoenzyme analysis in the French National Reference Center for Leishmaniasis in Montpellier (25).…”

Section: Methodsmentioning

confidence: 99%

Heterogeneity of Molecular Resistance Patterns in Antimony-Resistant Field Isolates of Leishmania Species from the Western Mediterranean Area

Jeddi

Mary

Karim

et al. 2014

Antimicrob Agents Chemother

View full text Add to dashboard Cite

h Antimonials remain the first-line treatment for the various manifestations of leishmaniasis in most areas where the disease is endemic, and increasing cases of therapeutic failure associated with parasite resistance have been reported. In this study, we assessed the molecular status of 47 clinical isolates of Leishmania causing visceral and cutaneous leishmaniasis from Algeria, Tunisia, and southern France. In total, we examined 14 genes that have been shown to exhibit significant variations in DNA amplification, mRNA levels, or protein expression with respect to resistance to antimonials. The gene status of each clinical isolate was assessed via qPCR and qRT-PCR. We then compared the molecular pattern against the phenotype determined via an in vitro sensitivity test of the clinical isolates against meglumine antimoniate, which is considered the reference technique. Our results demonstrate significant DNA amplification and/or RNA overexpression in 56% of the clinical isolates with the resistant phenotype. All clinical isolates that exhibited significant overexpression of at least 2 genes displayed a resistant phenotype. Among the 14 genes investigated, 10 genes displayed either significant amplification or overexpression in at least 1 clinical isolate; these genes are involved in several metabolic pathways. Moreover, various gene associations were observed depending on the clinical isolates, supporting the multifactorial nature of Leishmania resistance. Molecular resistance features were found in the 3 Leishmania species investigated (Leishmania infantum, Leishmania major, and Leishmania killicki). To our knowledge, this is the first report of the involvement of molecular resistance genes in field isolates of Leishmania major and Leishmania killicki with the resistance phenotype.

show abstract

“…PCR and subsequent sequencing of DNA products can permit the species identification of samples for which parasite culture remained negative or did not allow isoenzymatic characterization [118]. The multilocus sequence typing (MLST) and the Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry are promising approaches, providing rapid and accurate identification of Leishmania from in vitro culture at the species level [119,120].…”

Section: Cutaneous and Mucosal Leishmaniasismentioning

confidence: 99%

Leishmaniasis in travelers: A literature review

Mansueto

Seidita

Vitale

et al. 2014

Travel Medicine and Infectious Disease

112

View full text Add to dashboard Cite

Summary Leishmaniasis is a vector-borne protozoan infection whose clinical spectrum ranges from asymptomatic infection to fatal visceral leishmaniasis. Over the last decades, an increase in imported leishmaniasis cases in developed, non-endemic countries, have been pointed-out from a review of the international literature. Among the possible causes are increasing international tourism, influx of immigrants from endemic regions and military operations. The main area for the acquisition of cutaneous leishmaniasis, especially for adventure travelers on long-term trips in highly-endemic forested areas, is represented from South America, whereas popular Mediterranean destinations are emerging as the main areas to acquire visceral variant. Leishmaniasis should be considered in the diagnostic assessment of patients presenting with a compatible clinical syndrome and a history of travel to an endemic area, even if this occurred several months or years before. Adventure travelers, researchers, military personnel, and other groups of travelers likely to be exposed to sand flies in endemic areas, should receive counseling regarding leishmaniasis and appropriate protective measures. ª

show abstract

Identification of Leishmania at the species level with matrix-assisted laser desorption ionization time-of-flight mass spectrometry

Cited by 35 publications

References 16 publications

Species Typing in Dermal Leishmaniasis

Species Typing in Dermal Leishmaniasis

Heterogeneity of Molecular Resistance Patterns in Antimony-Resistant Field Isolates of Leishmania Species from the Western Mediterranean Area

Leishmaniasis in travelers: A literature review

Contact Info

Product

Resources

About