Identification of leaf rust resistance genes in wheat (Triticum aestivum L.) cultivars using molecular markers

Urbanovich, O. Yu.; Малышев, С. В.; Dolmatovich, T. V.; Kartel, N. A.

doi:10.1134/s1022795406050127

Cited by 20 publications

(11 citation statements)

References 36 publications

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“…Despite its low frequency in local wheat cultivars, the wide range of effectiveness positioned Lr9 as a useful source of resistance when deployed in combination with complementary Lr genes like Lr51, Lr21, etc. Lr9, derived from Triticum umbellulata, has also been detected in low frequency in some European countries and in the USA (Urbanovich et al 2006;Kolmer et al 2007a). …”

Section: Seedling Resistance Genes Detected In Adapted Cultivars Usinmentioning

confidence: 95%

Identification of leaf rust resistance genes i n selected Argentinean bread wheat cultivars by gene postulation and molecular markers

Vanzetti¹,

Campos²,

Demichelis³

et al. 2011

Electro Journal of Biotech

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Leaf rust, caused by Puccinia triticina Eriks. is a common and widespread disease of bread wheat (Triticum aestivum L.), in Argentina. Host resistance is the most economical, effective and ecologically sustainable method of controlling the disease. Gene postulation helps to determine leaf rust resistance genes (Lr genes) that may be present in a large group of wheat germplasm. Additionally presence of Lr genes can be determined using associated molecular markers. The objective of this study was to identify Lr genes that condition leaf rust resistance in 66 wheat cultivars from Argentina. Twenty four differential lines with individual known leaf rust resistance genes were tested with 17 different pathotypes of leaf rust collected from Argentina. Leaf rust infection types produced on seedling plants of the 66 local cultivars were compared with the infection types produced by the same pathotypes on Lr differentials to postulate which seedling leaf rust genes were present. Presence of Lr9, Presence of Lr21, Lr25, Lr29, and Lr47 could not be determined with the seventeen pathotypes used in the study because all were avirulent to these genes. Eleven cultivars (16.7%) were resistant to all pathotypes used in the study and the remaining 55 (83.3%) showed virulent reaction against one or more local pathotypes. Cultivars with seedling resistance gene combinations including Lr16 or single genes Lr47 (detected with molecular marker), Lr19 and Lr41, showed high levels of resistance against all pathotypes or most of them. On the opposite side, cultivars with seedling resistance genes Lr1, Lr3a, Lr3a + Lr24, Lr10, Lr3a + Lr10, Lr3a + Lr10 + Lr24 showed the highest number of virulent reactions against local pathotypes. Occurrence of adult plant resistance genes Lr34, Lr35 and Lr37 in local germplasm was evaluated using specific molecular markers confirming presence of Lr34 and Lr37. Our data suggest that combinations including seedling resistance genes like Lr16, Lr47, Lr19, Lr41, Lr21, Lr25 and Lr29, with adult plant resistance genes like Lr34, SV2, Lr46 will probably provide durable and effective resistance to leaf rust in the region.

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Section: Seedling Resistance Genes Detected In Adapted Cultivars Usinmentioning

confidence: 95%

Identification of leaf rust resistance genes i n selected Argentinean bread wheat cultivars by gene postulation and molecular markers

Vanzetti¹,

Campos²,

Demichelis³

et al. 2011

Electro Journal of Biotech

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“…For instance, the cosegregating RFLP marker Lrk10 was converted into a PCRbased sequence-tagged-site (STS) marker for the gene Lr10 (Schachermayr et al 1997) and Naik et al (1998) converted the random ampliWed polymorphic DNA (RAPD) marker OPJ-01 378 into an STS marker for the gene Lr28. Seyfarth et al (1999) also developed an STS marker for the gene Lr35 through cloning and sequencing of a cosegregating RFLP probe and, currently, Urbanovich et al (2006) developed and applied DNA marker assays to select leaf rust resistance at nine loci in wheat.…”

Section: Discussionmentioning

confidence: 99%

Mapping of the leaf rust resistance gene Lr38 on wheat chromosome arm 6DL using SSR markers

et al. 2007

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Leaf rust caused by the fungus Puccinia triticina is one of the most important diseases of wheat (Triticum aestivum) worldwide. The use of resistant wheat cultivars is considered the most economical and environment-friendly approach in controlling the disease. The Lr38 gene, introgressed from Agropyron intermedium, confers a stable seedling and adult plant resistance against multiple isolates tested in Europe. In the present study, 94 F 2 plants resulting from a cross made between the resistant Thatcherderived near-isogenic line (NIL) RL6097, and the susceptible Ethiopian wheat cultivar Kubsa were used to map the Thatcher Lr38 locus in wheat using simple sequence repeat (SSR) markers. Out of 54 markers tested, 15 SSRs were polymorphic between the two parents and subsequently genotyped in the population. The P. triticina isolate DZ7-24 (race FGJTJ), discriminating Lr38 resistant and susceptible plants, was used to inoculate seedlings of the two parents and the segregating population. The SSR markers Xwmc773 and Xbarc273 Xanked the Lr38 locus at a distance of 6.1 and 7.9 cM, respectively, to the proximal end of wheat chromosome arm 6DL. The SSR markers Xcfd5 and Xcfd60 both Xanked the locus at a distance of 22.1 cM to the distal end of 6DL. In future, these SSR markers can be used by wheat breeders and pathologists for marker assisted selection (MAS) of Lr38-mediated leaf rust resistance in wheat.

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“…tritici, is the most common fungal disease occurring in all regions where winter and spring wheat is cultivated (Vanzetti et al, 2011;Abdelbacki et al, 2013). Every year wheat leaf rust damages more than 10% of crops (Prabhu et al, 2004;Urbanovich et al, 2006), but severe infestation may affect as much as 40% of crops (Kassem et al, 2011). In Poland on average 9.1% of crops is lost to wheat leaf rust annually (Trawal, Walczak, 2012).…”

Section: Introductionmentioning

confidence: 99%

An analysis of the functionality of molecular markers related to the Lr19 gene conditioning resistance to wheat leaf rust

Kiel

Weigt

Karpińska

et al. 2020

Zemdirbyste-Agriculture

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Among all cereals, common wheat (Triticum aestivum L.) occupies the largest area of crops worldwide. Wheat leaf rust, caused by the pathogen Puccinia recondita f. sp. tritici considerably reduces the yield of wheat. Breeding for resistance combined with selection based on molecular markers may become an effective tool in the struggle against fungal diseases. Lr19 is the gene that carries high resistance to wheat leaf rust. In recent years, we have seen the development of many molecular markers in close neighbourhood of the Lr19 gene, e.g., SCS265, SCS253, GB, Xwmc221, XustSSR2001-7DL, Xgwm37 and Xgwm44. The aim of the study was to investigate the functionality of molecular markers related to the Lr19 gene. The study was based on two reference genotypes with the Lr19 gene ('Agatha' and Lr19) and three lines strongly infested by wheat leaf rust. Seven molecular markers were analysed, but only two of them (GB and Xwmc221) proved to be specific to the gene under study. These markers were used for analysis of 25 wheat genotypes, which were evaluated for leaf rust resistance in field conditions to confirm their usefulness for selection of breeding material. These findings point to the need of continuous search for functional molecular markers giving repeatable and reliable results.

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Identification of leaf rust resistance genes in wheat (Triticum aestivum L.) cultivars using molecular markers

Cited by 20 publications

References 36 publications

Identification of leaf rust resistance genes i n selected Argentinean bread wheat cultivars by gene postulation and molecular markers

Identification of leaf rust resistance genes i n selected Argentinean bread wheat cultivars by gene postulation and molecular markers

Mapping of the leaf rust resistance gene Lr38 on wheat chromosome arm 6DL using SSR markers

An analysis of the functionality of molecular markers related to the Lr19 gene conditioning resistance to wheat leaf rust

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