Identification of KLF13 and KLF14 (SP6), Novel Members of the SP/XKLF Transcription Factor Family

Scohy, Sophie; Gabant, Philippe; Reeth, Thierry Van; Hertveldt, Valérie; Dreze, Pierre Luc; Vooren, Pascale Van; Riviere, Michèle; Szpirer, Josiane; Szpirer, Claude

doi:10.1006/geno.2000.6362

Cited by 72 publications

(72 citation statements)

References 45 publications

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“…Donor and acceptor sequences are shown (small letters for exon sequences, and large letters for the intron sequence). The broken box and dotted line represent the previously predicted exon 1 and promoter region of the Sp6/KFL14 gene (1). B, the promoter sequence and exon 1 and 2 sequences with translated amino acids.…”

Section: Resultsmentioning

confidence: 99%

“…The coding sequence of Sp6/KLF14 cDNA was previously predicted on the basis of composite sequences from the mouse gene and expressed sequence tags (1). To obtain the 5Ј end of epiprofin cDNA to compare it with Sp6/KLF14 cDNA, we performed 5Ј Cap rapid amplification of cDNA ends PCR using mouse E18 molar RNA with specific extension primers for epiprofin and cloned into the pCR4-TOPO vector.…”

Section: Resultsmentioning

confidence: 99%

“…Although epiprofin cDNA was isolated from a molar tooth germ cDNA library by differential screening, it was encoded by a gene that had previously been reported to code for Sp6/ KLF14, a new member of the Krü ppel-like protein family identified by a zinc finger motif search through GenBank TM (1). The full-length epiprofin cDNA is different in its 5Ј sequence from the proposed 5Ј sequence of the mouse Sp6/KLF14 cDNA derived from composites of two expressed sequence tags and the mouse gene.…”

Section: Discussionmentioning

confidence: 99%

“…Some of these proteins, such as Sp1 and BTEB1/KLF9, are ubiquitously expressed (11,12), whereas others, such as EKLF/KLF1 and LKLF/KLF2, are expressed in a tissue-specific manner (13,14). KLF13 and KLF14/Sp6 were identified by screening a mouse expressed sequence tag data base using the Sp1 zinc finger domain as a probe and were reported to be ubiquitous by RT-PCR analysis (1). Sp/KLF protein factors regulate a wide range of cellular functions including cell growth, differentiation, apoptosis, and tumor formation.…”

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confidence: 99%

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The Krüppel-like Factor Epiprofin Is Expressed by Epithelium of Developing Teeth, Hair Follicles, and Limb Buds and Promotes Cell Proliferation

Nakamura

Unda

de-Vega

et al. 2004

Journal of Biological Chemistry

112

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We identified a cDNA clone for epiprofin, which is preferentially expressed in teeth, by differential hybridization using DNA microarrays from an embryonic day 19.5 mouse molar cDNA library. Sequence analysis revealed that this cDNA encodes a member of the Krü ppellike factor family containing three characteristic C 2 H 2 -type zinc finger motifs. The full-length cDNA was obtained by the 5 Cap capture method. Except for its 5 -terminal sequence, the epiprofin mRNA sequence is almost identical to the predicted sequence of Krü ppellike factor 14/Sp6 (specificity protein 6), which was previously identified in expressed sequence tag data bases and GenBank TM by an Sp1 zinc finger DNA-binding domain search (Scohy, S., Gabant, P., Van Reeth, T., Hertveldt, V., Dreze, P. L., Van Vooren, P., Riviere, M., Szpirer, J., and Szpirer, C. (2000) Genomics 70, 93-101). This sequence difference is due to differences in the assignment of the location of exon 1. In situ hybridization revealed that epiprofin mRNA is expressed by proliferating dental epithelium, differentiated odontoblast, and also hair follicle matrix epithelium. In addition, whole mount in situ hybridization showed transient expression of epiprofin mRNA in cells of the apical ectodermal ridge in developing limbs and the posterior neuropore. Transfection of an epiprofin expression vector revealed that this molecule is localized in the nucleus and promotes cell proliferation. Thus, epiprofin is a highly cell-and tissue-specific nuclear protein expressed primarily by proliferating epithelial cells of teeth, hair follicles, and limbs that may function in the development of these tissues by regulating cell growth.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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The Krüppel-like Factor Epiprofin Is Expressed by Epithelium of Developing Teeth, Hair Follicles, and Limb Buds and Promotes Cell Proliferation

Nakamura

Unda

de-Vega

et al. 2004

Journal of Biological Chemistry

112

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“…Low-level detection of KLF13 transcripts has been detected by RT-PCR in other adult mouse tissues [49] although the full significance of this finding is unclear. In the mouse embryo, there is robust developmental expression of KLF13 in the heart, cephalic mesenchyme, dermis, and epithelial layers of the gut and urinary bladder [50].…”

Section: Klf13mentioning

confidence: 99%

Kruppel-like Factors (KLFs) in muscle biology

Haldar¹,

Ibrahim²,

Jain³

2007

Journal of Molecular and Cellular Cardiology

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The Kruppel-like Factor (KLF) family of zinc-finger transcription factors are critical regulators of cell differentiation, phenotypic modulation and physiologic function. An emerging body of evidence implicates an important role for these factors in cardiovascular biology, however, the role of KLFs in muscle biology is only beginning to be understood. This article reviews the published data describing the role of KLFs in cardiomyocytes, smooth muscle cells, and skeletal muscle and highlights the importance of these factors in cardiovascular development, physiology and disease pathobiology.

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MicroRNA‐125a contributes to elevated inflammatory chemokine RANTES levels via targeting KLF13 in systemic lupus erythematosus

Zhao

Tang

et al. 2010

Arthritis & Rheumatism

223

151

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Objective. MicroRNA (miRNA) have received increasing attention as posttranscriptional regulators that fine-tune the homeostasis of the inflammatory response. This study aimed to clarify whether miR-125a, which was identified in a pilot expression profiling step, is involved in the inflammatory chemokine pathway in systemic lupus erythematosus (SLE).Methods. Independent verification of miR-125a expression in amplified samples from SLE patients and normal controls was performed by TaqMan quantitative polymerase chain reaction (PCR) analysis. A combination of 3 bioinformatic prediction techniques and reporter gene assays was used to identify miR-125a targets. In vitro systems of overexpression by transfection and inducible expression by stimulation were performed to investigate the function of miR-125a, which was followed by real-time quantitative PCR and enzymelinked immunosorbent assay.Results. In SLE patients, the expression of miR125a was reduced and the expression of its predicted target gene, KLF13, was increased. Bioinformatics predicted that miR-125a base-paired with sequences in the 3 -untranslated region of KLF13. Overexpression of miR-125a led to a significant reduction in the expression of RANTES and KLF13. MicroRNA-125a inhibited endogenous KLF13 expression in a dose-dependent manner, as determined using gain-and loss-of-function methods. A luciferase reporter system confirmed the miR-125a binding sites. Notably, miR-125a expression was induced in T cells in a dose-and time-dependent manner. Finally, the introduction of miR-125a into T cells from SLE patients alleviated the elevated RANTES expression.Conclusion. MicroRNA-125a negatively regulates RANTES expression by targeting KLF13 in activated T cells. The underexpression of miR-125a contributes to the elevated expression of RANTES in SLE. Our findings extend the role of miRNA in the pathogenesis of lupus and provide potential strategies for therapeutic intervention.

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Identification of KLF13 and KLF14 (SP6), Novel Members of the SP/XKLF Transcription Factor Family

Cited by 72 publications

References 45 publications

The Krüppel-like Factor Epiprofin Is Expressed by Epithelium of Developing Teeth, Hair Follicles, and Limb Buds and Promotes Cell Proliferation

The Krüppel-like Factor Epiprofin Is Expressed by Epithelium of Developing Teeth, Hair Follicles, and Limb Buds and Promotes Cell Proliferation

Kruppel-like Factors (KLFs) in muscle biology

MicroRNA‐125a contributes to elevated inflammatory chemokine RANTES levels via targeting KLF13 in systemic lupus erythematosus

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