Identification of Key Genes Associated with Heat Stress in Rats by Weighted Gene Co-Expression Network Analysis

Zhang, Fan; Dou, Junling; Zhao, Xiuxin; Luo, Hanpeng; Ma, Longgang; Wang, Lei; Wang, Yachun

doi:10.3390/ani13101618

Cited by 1 publication

(5 citation statements)

References 54 publications

(65 reference statements)

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“…Research shows that transcriptome can help researchers analyze which pathways and genes are activated in response to the stressor ( Zhang Y. et al, 2023 ; Zhang F. et al, 2023 ; Zhang X. et al, 2023 ). Compared to other parts of the intestine, the cecum of broilers plays a more important role in host defense ( Khan and Chousalkar, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Metabolomics research can understand changes in the metabolism of organisms, helping researchers better understand how chicken products are affected by the external environment ( Zhang Y. et al, 2023 ; Zhang F. et al, 2023 ; Zhang X. et al, 2023 ). The fermentation products produced by cecal microorganisms have a positive impact on intestinal health, and numerous cecal metabolites play a crucial role in maintaining intestinal barrier function ( Liu et al, 2022 , 2022a , b ).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the oxidative damage caused by heat stress also reflects the reduction of ascorbyl palmitate content. Zhang Y. et al (2023) , Zhang F. et al (2023) , and Zhang X. et al (2023) showed that the increase of ADP ribose content can identify damaged DNA and further activate the base excision repair mechanism to participate in heat stress regulation. This also confirms the upregulation of ADP ribose metabolite content in this study.…”

Section: Discussionmentioning

confidence: 99%

“…It can build a direct correlation between metabolites and biological phenotypes, dynamically track and analyze the metabolites of animal bodies, help to analyze the relationship between phenotypes and genetics, environmental and other factors, and provide a basis for the improvement and breeding of economic traits. Metabolomics is widely used to understand the changes in organism metabolism, such as metabolism, sugar, lipid, amino acid metabolism, and meat quality evaluation ( Zhang Y. et al, 2023 ; Zhang F. et al, 2023 ; Zhang X. et al, 2023 ). Zampiga et al (2021) found that chronic heat stress could regulate the changes of metabolites in breast muscle and plasma of broilers by metabolome, and found metabolites that might play an important role in regulating Energy homeostasis of the body.…”

Section: Introductionmentioning

confidence: 99%

“…The cecum is the broiler gastrointestinal tract’s most diverse area of microbes and various microbes are crucial in improving growth performance and maintaining physical health ( Luo et al, 2021 ). Previous studies mainly explored the effects of heat stress on broiler intestines from a single omics perspective, including transcriptome, metabolomics, and microbiome ( Dridi et al, 2022 ; Kim et al, 2022 ; Zhang Y. et al, 2023 ; Zhang F. et al, 2023 ; Zhang X. et al, 2023 ). However, very few studies have comprehensively revealed the heat-stress impact on broilers’ guts.…”

Section: Introductionmentioning

confidence: 99%

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Heat stress exposure cause alterations in intestinal microbiota, transcriptome, and metabolome of broilers

Liu,

Ma,

Wang

et al. 2023

Front. Microbiol.

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IntroductionHeat stress can affect the production of poultry through complex interactions between genes, metabolites and microorganisms. At present, it is unclear how heat stress affects genetic, metabolic and microbial changes in poultry, as well as the complex interactions between them.MethodsThus, at 28 days of age a total of 200 Arbor Acres broilers with similar body weights were randomly divided into the control (CON) and heat stress treatment (HS). There were 5 replicates in CON and HS, respectively, 20 per replication. From the 28–42 days, the HS was kept at 31 ± 1°C (9:00–17:00, 8 h) and other time was maintained at 21 ± 1°C as in the CON. At the 42nd day experiment, we calculated the growth performance (n = 8) of broilers and collected 3 and 6 cecal tissues for transcriptomic and metabolomic investigation and 4 cecal contents for metagenomic investigation of each treatment.Results and discussionThe results indicate that heat stress significantly reduced the average daily gain and body weight of broilers (value of p < 0.05). Transcriptome KEGG enrichment showed that the differential genes were mainly enriched in the NF-kB signaling pathway. Metabolomics results showed that KEGG enrichment showed that the differential metabolites were mainly enriched in the mTOR signaling pathway. 16S rDNA amplicon sequencing results indicated that heat stress increased the relative abundance of Proteobacteria decreased the relative abundance of Firmicutes. Multi-omics analysis showed that the co-participating pathway of differential genes, metabolites and microorganisms KEGG enrichment was purine metabolism. Pearson correlation analysis found that ornithine was positively correlated with SULT1C3, GSTT1L and g_Lactobacillus, and negatively correlated with CALB1. PE was negatively correlated with CALB1 and CHAC1, and positively with g_Alistipes. In conclusion, heat stress can generate large amounts of reactive oxygen and increase the types of harmful bacteria, reduce intestinal nutrient absorption and antioxidant capacity, and thereby damage intestinal health and immune function, and reduce growth performance indicators. This biological process is manifested in the complex regulation, providing a foundational theoretical basis for solving the problem of heat stress.

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