Identification of Key Genes and Pathways Involved in the Heterogeneity of Intrinsic Growth Ability Between Neurons After Spinal Cord Injury in Adult Zebrafish

Fu, Hua; Han, Gonghai; Li, Haojiang; Liang, Xue‐Zhen; Hu, Die; Zhang, Licheng; Tang, Peifu

doi:10.1007/s11064-019-02841-1

Cited by 8 publications

(3 citation statements)

References 39 publications

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“…Zebrafish studies have already yielded valuable information on potential candidate proteins involved in neuroregeneration following SCIs, such as CTGFa [ 132 ], Cav1 [ 51 ], ANP32a [ 133 ], matrix metalloproteinase-9 [ 158 ], and sonic hedgehog [ 159 , 160 ]. Furthermore, in the current zebrafish study, differentially expressed genes were sorted and analyzed in-depth using bioinformatics methods, such as the Notch signaling pathway [ 161 , 162 ], Wnt signaling pathway [ 163 ], and Hippo-Yap/Taz signaling pathway [ 164 ]. These results suggest that, after an SCI in adult zebrafish, our understanding of the molecular mechanisms underlying axon regeneration can be facilitated, and these candidate genes and pathways can serve as therapeutic targets for the treatment of CNS injuries.…”

Section: Future Directions: Zebrafish Neuron Regeneration Research In...mentioning

confidence: 99%

The Promising Role of a Zebrafish Model Employed in Neural Regeneration Following a Spinal Cord Injury

Zeng,

Tsai

2023

IJMS

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Spinal cord injury (SCI) is a devastating event that results in a wide range of physical impairments and disabilities. Despite the advances in our understanding of the biological response to injured tissue, no effective treatments are available for SCIs at present. Some studies have addressed this issue by exploring the potential of cell transplantation therapy. However, because of the abnormal microenvironment in injured tissue, the survival rate of transplanted cells is often low, thus limiting the efficacy of such treatments. Many studies have attempted to overcome these obstacles using a variety of cell types and animal models. Recent studies have shown the utility of zebrafish as a model of neural regeneration following SCIs, including the proliferation and migration of various cell types and the involvement of various progenitor cells. In this review, we discuss some of the current challenges in SCI research, including the accurate identification of cell types involved in neural regeneration, the adverse microenvironment created by SCIs, attenuated immune responses that inhibit nerve regeneration, and glial scar formation that prevents axonal regeneration. More in-depth studies are needed to fully understand the neural regeneration mechanisms, proteins, and signaling pathways involved in the complex interactions between the SCI microenvironment and transplanted cells in non-mammals, particularly in the zebrafish model, which could, in turn, lead to new therapeutic approaches to treat SCIs in humans and other mammals.

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Section: Future Directions: Zebrafish Neuron Regeneration Research In...mentioning

confidence: 99%

The Promising Role of a Zebrafish Model Employed in Neural Regeneration Following a Spinal Cord Injury

Zeng,

Tsai

2023

IJMS

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“…This scarring creates an unfavorable environment for tissue renewal [ 8 ]. Experiments in non-mammalian species, particularly fishes and amphibians, have shown some peculiarities that may account for the increased regenerative potential of these species [ 9 , 10 , 11 , 12 , 13 ]. Thus, investigators have probed the gene expression profiles of species that can regenerate their spinal cord following transection, as a source of insight (and future therapies) to understand the limits of CNS regeneration in mammals.…”

Section: Introductionmentioning

confidence: 99%

The Stress Response of the Holothurian Central Nervous System: A Transcriptomic Analysis

Cruz-González

Quesada-Díaz

Miranda-Negrón

et al. 2022

IJMS

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Injury to the central nervous system (CNS) results in permanent damage and lack of function in most vertebrate animals, due to their limited regenerative capacities. In contrast, echinoderms can fully regenerate their radial nerve cord (RNC) following transection, with little to no scarring. Investigators have associated the regenerative capacity of some organisms to the stress response and inflammation produced by the injury. Here, we explore the gene activation profile of the stressed holothurian CNS. To do this, we performed RNA sequencing on isolated RNC explants submitted to the stress of transection and enzyme dissection and compared them with explants kept in culture for 3 days following dissection. We describe stress-associated genes, including members of heat-shock families, ubiquitin-related pathways, transposons, and apoptosis that were differentially expressed. Surprisingly, the stress response does not induce apoptosis in this system. Other genes associated with stress in other animal models, such as hero proteins and those associated with the integrated stress response, were not found to be differentially expressed either. Our results provide a new viewpoint on the stress response in the nervous system of an organism with amazing regenerative capacities. This is the first step in deciphering the molecular processes that allow echinoderms to undergo fully functional CNS regeneration, and also provides a comparative view of the stress response in other organisms.

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“…This scarring creates a milieu unfavorable for tissue renewal [8]. Experiments in non-mammalian species, particularly fishes and amphibians, have shown some peculiarities that may account for the increased regenerative potential of these species [9][10] [11][12] [13]. Thus, investigators have probed the gene expression profiles of species that can regenerate their spinal cord, following transection, as a source of insights (and future therapies) to understand the limits of CNS regeneration in mammals.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of the Stress Response of the Holothurian Central Nervous System

Cruz-González¹,

Quesada-Díaz²,

Miranda-Negrón³

et al. 2022

Preprint

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Injury to the central nervous system (CNS), in most vertebrate animals, results in permanent damage and lack of function, due to their limited regenerative capacities. In contrast, echinoderms can fully regenerate their radial nerve cord (RNC) following transection, with little or no scarring. Investigators have associated the regenerative capacity of some organisms with the stress response and inflammation produced by the injury. Here we explore the gene activation profile of the stressed holothurian CNS. To do this, we performed RNA sequencing on isolated RNC explants submitted to the stress of transection and enzyme dissection and compared them to explants kept in culture for 3 days following dissection. We describe stress-associated genes, including members of heat-shock families, ubiquitin-related pathways, transposons, and apoptosis that were differentially expressed. Surprisingly, the stress response does not induce apoptosis in this system. Other genes associated with stress in other animal models, such as hero proteins and those associated with the integrated stress response, were not found to be differentially expressed either. Our results provide a new viewpoint on the stress response in the nervous system of an organism with an amazing regenerative capacity. This is the first step to deciphering the molecular processes that allow echinoderms to undergo fully functional CNS regeneration while also providing a comparative view for students of the stress response in other organisms.

show abstract

Identification of Key Genes and Pathways Involved in the Heterogeneity of Intrinsic Growth Ability Between Neurons After Spinal Cord Injury in Adult Zebrafish

Cited by 8 publications

References 39 publications

The Promising Role of a Zebrafish Model Employed in Neural Regeneration Following a Spinal Cord Injury

The Promising Role of a Zebrafish Model Employed in Neural Regeneration Following a Spinal Cord Injury

The Stress Response of the Holothurian Central Nervous System: A Transcriptomic Analysis

Molecular Characterization of the Stress Response of the Holothurian Central Nervous System

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