Identification of key biomarkers in steroid-induced osteonecrosis of the femoral head and their correlation with immune infiltration by bioinformatics analysis

Zhao, Jun; Zhang, Xingshi; Guan, Junjie; Su, Yu; Jiang, Ji-Zhao

doi:10.1186/s12891-022-04994-7

Cited by 10 publications

(11 citation statements)

References 53 publications

(42 reference statements)

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“…Microsoft Excel was used to segregate the relevant physiological data and tables. Barplot in R (4.0.2) was used to draw the histogram (mean ± standard error (SE); SE = SD/sqrt (n)), cor to draw the correlation analysis chart (Zhao et al, 2022), and gplots and pheatmap to draw the heat map after the FPKM values of gene expression in three time periods were standardized.…”

Section: Data Processing and Analysismentioning

confidence: 99%

Effects of light spectrum on the morphophysiology and gene expression of lateral branching in Pepino (Solanum muricatum)

Yang

Lou

et al. 2022

Front. Plant Sci.

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In the present study, we determined the morphological and physiological indicators of Pepino to elucidate its lateral branching responses to different light qualities using a full-spectrum lamp (F) as the control and eight different light ratios using blue light (B) and red light (R). In addition, correlation analysis revealed that the gene expression patterns correlated with lateral branching under various light treatments. Compared with the F treatment, the R treatment increased the plant height and inhibited the elongation of lateral branches, in contrast with the B treatment. The number of lateral branches did not change significantly under different light quality treatments. Moreover, correlation analysis showed that the ratio of blue light was significantly positively correlated with the length of lateral branches and significantly negatively correlated with plant height, aboveground dry weight, and other indicators. We conducted transcriptome sequencing of the sites of lateral branching at three periods under different light quality treatments. The gene related to photodynamic response, cryptochrome (CRY), was the most highly expressed under B treatment, negatively regulated lateral branch length, and positively correlated with plant height. Branched 1, a lateral branch regulation gene, was upregulated under R treatment and inhibited branching. Overall, the red light facilitated internode elongation, leaf area expansion, plant dry weight increase, and inhibition of lateral branching. Soluble sugar content increased, and the lateral branches elongated under blue light. Different light qualities regulated lateral branching by mediating different pathways involving strigolactones and CRY. Our findings laid a foundation for further clarifying the response mechanism of Pepino seedlings to light and provided a theoretical reference for elucidating the regulation of different light qualities on the lateral branching of Pepino.

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Section: Data Processing and Analysismentioning

confidence: 99%

Effects of light spectrum on the morphophysiology and gene expression of lateral branching in Pepino (Solanum muricatum)

Yang

Lou

et al. 2022

Front. Plant Sci.

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“…Therefore, we hypothesize that in the pathogenesis of SONFH, an imbalance in copper metabolism may cause disordered immune regulation in the body and thus induce femoral head necrosis. Recently, studies have shown that immune cell infiltration is associated with the progression of SONFH [ 35 , 60 , 61 ]. In the present study, we concluded that PNP expression had a notable and the strongest negative association with plasmacytoid dendritic cell infiltration, while PNP expression had a notable and the strongest positive association with CD56 bright natural killer cell infiltration.…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics-Based Analysis of Key Genes in Steroid-Induced Osteonecrosis of the Femoral Head That Are Associated with Copper Metabolism

Cai

et al. 2023

Biomedicines

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Osteonecrosis of the femoral head (ONFH) is a common disabling disease. Copper has positive effects on cells that regulate bone metabolism. However, the relationship between copper metabolism (CM) and steroid-induced ONFH (SONFH) remains unclear. The GSE123568 dataset was downloaded from the Gene Expression Omnibus. The differentially expressed CM-related SONFH genes (DE-CMR-SONFHGs) were identified via differential analysis and weighted gene coexpression network analysis (WGCNA). Receiver operating characteristic (ROC) analysis was performed for the predictive accuracy of key genes. Targeting drugs and the copper death-related genes (CDRGs) relevant to key genes were investigated. The bioinformatics results were confirmed via quantitative real-time polymerase chain reaction (qRT–PCR) and Western blot (WB) analysis. Two out of 106 DE-CMR-SONFHGs were identified as key genes (PNP and SLC2A1), which had diagnostic value in distinguishing SONFH from control samples and were related to various immune cell infiltrations. Eleven PMP-targeting drugs and five SLC2A1-targeting drugs were identified. The qRT–PCR, as well as WB, results confirmed the downregulation PNP and SLC2A1 and high expression of the CDRGs DLD, PDHB, and MTF1, which are closely related to these two key genes. In conclusion, PNP and SLC2A1 were identified as key genes related to SONFH and may provide insights for SONFH treatment.

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“…64 During the progression of GIONFH, large numbers of chronic inflammatory cells may infiltrate into the peripheral blood and necrotic zone. 65 Multiple studies [66][67][68] evaluated immune infiltration in serum by analyzing GSE123568 which enrolled thirty patients with GIONFH and ten healthy controls, and found decreased infiltration of memory B cells and activated dendritic cells. It could be noticed that macrophages (M0, M1 and M2) were not significantly enriched in the GIONFH group.…”

Section: Immune Infiltration and Macrophage Distribution In Gionfh Im...mentioning

confidence: 99%

Polarization Behavior of Bone Macrophage as Well as Associated Osteoimmunity in Glucocorticoid-Induced Osteonecrosis of the Femoral Head

Zhang¹,

Sun²,

Li³

et al. 2023

JIR

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Glucocorticoid-induced osteonecrosis of the femoral head (GIONFH) is a disabling disease with high mortality in China but the detailed molecular and cellular mechanisms remain to be investigated. Macrophages are considered the key cells in osteoimmunology, and the cross-talk between bone macrophages and other cells in the microenvironment is involved in maintaining bone homeostasis. M1 polarized macrophages launch a chronic inflammatory response and secrete a broad spectrum of cytokines (eg, TNF-α, IL-6 and IL-1β) and chemokines to initiate a chronic inflammatory state in GIONFH. M2 macrophage is the alternatively activated anti-inflammatory type distributed mainly in the perivascular area of the necrotic femoral head. In the development of GIONFH, injured bone vascular endothelial cells and necrotic bone activate the TLR4/NF-κB signal pathway, promote dimerization of PKM2 and subsequently enhance the production of HIF-1, inducing metabolic transformation of macrophage to the M1 phenotype. Considering these findings, putative interventions by local chemokine regulation to correct the imbalance between M1/M2 polarized macrophages by switching macrophages to an M2 phenotype, or inhibiting the adoption of an M1 phenotype appear to be plausible regimens for preventing or intervening GIONFH in the early stage. However, these results were mainly obtained by in vitro tissue or experimental animal model. Further studies to completely elucidate the alterations of the M1/M2 macrophage polarization and functions of macrophages in glucocorticoid-induced osteonecrosis of the femoral head are imperative.

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Identification of key biomarkers in steroid-induced osteonecrosis of the femoral head and their correlation with immune infiltration by bioinformatics analysis

Cited by 10 publications

References 53 publications

Effects of light spectrum on the morphophysiology and gene expression of lateral branching in Pepino (Solanum muricatum)

Effects of light spectrum on the morphophysiology and gene expression of lateral branching in Pepino (Solanum muricatum)

Bioinformatics-Based Analysis of Key Genes in Steroid-Induced Osteonecrosis of the Femoral Head That Are Associated with Copper Metabolism

Polarization Behavior of Bone Macrophage as Well as Associated Osteoimmunity in Glucocorticoid-Induced Osteonecrosis of the Femoral Head

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