IDENTIFICATION OF JAVAN LANGUR (Trachypithecus auratus) IN JAVAN LANGUR CENTER (JLC) COBAN TALUN, BATU BASED ON Cytochrome-b SEQUENCE

Abstract

Javan Langur (Trachypithecus auratus) is an endemic langur in Indonesia which classified as vulnerable primate. Some researches divided Javan Langur species into some subspecies, yet was not described properly. Cytochrome-b sequence from mitochondrial DNA is able to determine samples between and intraspecies as well. This study aimed to identify the Javan Langur (Trachypithecus auratus) samples named Andin (@&) and Bobby (B&), both are rehabilitated in Javan Langur Center (JLC). The amplification of Cytochrome-b gene from whole blood DNA with PCR technique using forward primer L151625'-CTTCCATGAGGACAAATATC-3' and reverse primer Rmuc15'-GTGGAGTATAGGTATGATTGC-3'. The phylogenetic tree reconstruction based on MEGA 5 using maximum likelihood (ML) method and pairwise distance analysis with Kimura-2 parameter model resulted that Andin and Bobby are in the same group with T. a. auratus. Andin is in the same clade with T. a. auratus haplotype aaF, while Bobby in the same clade T. a. auratus and T. a. auratus haplotype aaC. We concluded that both of them belong to T. a. auratus subspecies.

No longer based on photographs alone: refuting the validity of golden-crowned langur Presbytis johnaspinalli Nardelli 2015 (Mammalia, Primates, Cercopithecidae)

“…Roos et al (2008) sequenced 573 base pairs (bp) from 50 ebony langurs and found 19 haplotypes, labelling them aaA (T. auratus auratus A), aaB etc. For Bobby, 503 bp were sequenced confirming him to be an ebony langur with haplotype aaC (Rahmawati 2013;Rahmawati et al 2015). For Rus, at least 468 bp were successfully sequenced and it showed a one base-pair difference to haplotypes aaB, aaG and aaJ (Mushlih et al 2011), confirming her also to be an ebony langur.…”