Identification of Isomorphic Malaria Vectors using a DNA Probe

Panyim, Sakol; Yasothornsrikul, Sukkid; Tungpradubkul, Sumalee; Baimai, Visut; Rosenberg, Ronald; Andre, Richard G.; Green, C. A.

doi:10.4269/ajtmh.1988.38.47

Cited by 36 publications

(15 citation statements)

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“…dirus D. The species status of An. baimaii was confirmed by crossing experiments and chromosomal differences (Thu et al 1985(Thu et al , 1986a1988b), a DNA probe based on 7,000 recombinant clones derived from wild females (Panyim et al 1988b), a nonradioactive DNA probe method (Audtho et al 1995), nucleotide sequence of the ITS2 of rDNA (Xu & Qu 1997b;Xu et al 1998), allele-specific amplification in the nucleotide sequence of ITS2 (Walton et al 1999), a PCR based method (Huong et al 2001), and a SCAR marker and multiplex PCR-based method (Manguin et al 2002). Additionally, Green et al (1992) found support for the hypothesis that the four chromosomal / electrophoretic forms of the Dirus Complex represented four distinct biological species, and Damrongphol & Baimai (1989) pointed out diagnostic morphological characters of the eggs, useful for distinguishing An.…”

Section: Anopheles (Cellia) Baimaiimentioning

confidence: 93%

“…baimaii. More recently, recombinant DNA techniques, enzymatic tests, DNA probes, and DNA markers were developed to distinguish members of the Dirus Complex and confirm their species status (Panyim et al 1985(Panyim et al , 1988a(Panyim et al , 1988bGreen et al 1992;Audtho et al 1995;Xu & Qu 1997b;Xu et al 1998;Walton et al 1999;Huong et al 2001;Manguin et al 2002). Species of the Dirus Complex can be separated based on distinct patterns of major blocks of constitutive heterochromatin in the sex chromosomes and also banding patterns and densities of bands of the salivary gland polytene chromosomes (Baimai et al 1981;Baimai et al 1988b;Baimai et al 1988c;Wibowo et al 1984).…”

Section: Dirus Complexmentioning

confidence: 99%

See 1 more Smart Citation

Revision of the Leucosphyrus group of Anopheles (Cellia) (Diptera, Culicidae)

Sallum¹,

Peyton²,

Harrison³

et al. 2005

Rev. Bras. entomol.

101

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Section: Anopheles (Cellia) Baimaiimentioning

confidence: 93%

Section: Dirus Complexmentioning

confidence: 99%

Revision of the Leucosphyrus group of Anopheles (Cellia) (Diptera, Culicidae)

Sallum¹,

Peyton²,

Harrison³

et al. 2005

Rev. Bras. entomol.

101

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“…It is a straightforward approach and has been applied to several Anopheles species complexes, including the A. gambiae, A. dirus, A. quadrimaculatus and A. farauti complexes (e.9. Gale & Crampton, 1987a, b, 1988Panyim et a/., 1988;Cooper etal., 1991;Beebe etal., 1994). Speciesspecific sequences identified by this approach are used in diagnostic assays based on DNA hybridization.…”

Section: Methods For Distinguishing Between Cryptic Speciesmentioning

confidence: 99%

A review of the use of ribosomal DNA (rDNA) to differentiate among cryptic Anopheles species

Collins

Paskewitz

1996

Insect Molecular Biology

359

270

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Cryptic species complexes are groups of closely related species that are difficult or impossible to distinguish by morphological traits. These complexes are known from a wide variety of arthropods and are common among the well-studied, medically-important insects. For example, many of the anopheline vectors of malaria parasites are members of cryptic species complexes. Complexes typically include both vector and non-vector species, and two or more member species are often found syrnpatrically. Until the late 195Os, only two such Anopheles complexes were known, the A. gambiaecomplex from Africa and the A. maculipennis complex from Europe. Today, dozens of Anopheles cryptic species complexes are recognized, and accumulating evidence suggests that most important malaria vectors are likely to be members of such complexes.A variety of methods have been developed for identifying the species of individual specimens from these complexes, although until recently only those based on species-specific allozymes and polytene chromosome inversions were widely used. The limitations inherent in these methods have been circumvented with DNA-based procedures, which are especially useful because both sexes and all developmental stages can be identified, and DNA can be recovered from samples stored by a wide variety of simple methods. Several DNA-based identification techniques have been developed, including hybridization assays based on species-specific repeat sequences, and diagnostic PCR fragments produced

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“…These techniques include, in addition to the cytological examination of polytene chromosomes, other "traditional" tools such as genetic compatibility (7), isozyme analysis (8), and immunological procedures (9), and also methods based on recombinant DNA technology (10)(11)(12). Although the latter tools have proven useful, one major drawback of techniques based on DNA hybridization or sequence determination of specific genes is that they are time consuming.…”

mentioning

confidence: 99%

Polymorphisms detected by random PCR distinguish between different chromosomal forms of Anopheles gambiae.

Favia

Dimopoulos

Torre

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

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We have applied PCR amplification using random primers to distinguish between incipient species of the malaria vector Anopheles gambiae. Individuals belonging to three chromosomally characterized West African forms of this mosquito, which are important epidemiologically as they differ in vectorial capacity, were sampled both from laboratory stocks and from wild populations collected in three localities. The techniques used allowed for the unambiguous classification of the mosquitoes, providing a tool for rapid and efficient diagnosis, which previously relied on cytological examination of polytene chromosomes.

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Identification of Isomorphic Malaria Vectors using a DNA Probe

Cited by 36 publications

References 0 publications

Revision of the Leucosphyrus group of Anopheles (Cellia) (Diptera, Culicidae)

Revision of the Leucosphyrus group of Anopheles (Cellia) (Diptera, Culicidae)

A review of the use of ribosomal DNA (rDNA) to differentiate among cryptic Anopheles species

Polymorphisms detected by random PCR distinguish between different chromosomal forms of Anopheles gambiae.

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