Identification of isoforms of calyculin A-sensitive protein phosphatases which suppress full-type hyperactivation in bull ejaculated spermatozoa

Arai, Y.; Sakase, Mitsuhiro; Fukushima, Moriyuki; Harayama, Hiroshi

doi:10.1016/j.theriogenology.2019.02.010

Cited by 7 publications

(11 citation statements)

References 53 publications

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“…As hyperactivation is linked to sperm capacitation, it has been challenging to distinguish which processes of capacitation are important for sperm release. The discovery that several pharmacological agents could induce sperm hyperactivation in uncapacitated sperm allowed the evaluation of the effects of hyperactivation separately from other events that happen during sperm capacitation, such as membrane modifications 18 , 50 , 51 . Herein, we used compounds that can induce hyperactivation in sperm that are not capacitated, thus allowing the dissociation of capacitation and hyperactivation 51 .…”

Section: Discussionmentioning

confidence: 99%

“…For example, procaine 49 , 4-aminopyridine (which both activate CatSper indirectly) 49 , and the cell-permeable cAMP analog Sp-5,6-dichloro-1-b-D-ribofuranosyl-benzimidazole-30,50-monophosphorothioate induce full-type hyperactivation of porcine sperm 18 , 50 , which otherwise seldom undergo significant full-type hyperactivation in a capacitating medium, unlike mouse epididymal sperm 19 , 50 , 51 . Following the addition of these pharmacological agents, porcine sperm display a relatively high percentage of full-type hyperactivated motility before the time that sperm have completed capacitation 18 , 50 . This approach allows the separation of hyperactivation from other events in capacitation, which require 5–6 h 52 .…”

Section: Introductionmentioning

confidence: 99%

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Hyperactivation is sufficient to release porcine sperm from immobilized oviduct glycans

Sharif

Hickl

Juárez

et al. 2022

Sci Rep

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Fertilizing sperm are retained by adhesion to specific glycans on the epithelium of the oviduct forming a reservoir before sperm are released from the reservoir so fertilization can ensue. Capacitated sperm lose affinity for the oviduct epithelium but the components of capacitation that are important for sperm release are uncertain. One important correlate of capacitation is the development of hyperactivated motility. Hyperactivation is characterized by asymmetrical flagellar beating with high beat amplitude. We tested whether the development of full-type asymmetrical motility was sufficient to release sperm from immobilized oviduct glycans. Sperm hyperactivation was induced by four different compounds, a cell-permeable cAMP analog (cBiMPS), CatSper activators (4-aminopyridine and procaine), and an endogenous steroid (progesterone). Using standard analysis (CASA) and direct visualization with high-speed video microscopy, we first confirmed that all four compounds induced hyperactivation. Subsequently, sperm were allowed to bind to immobilized oviduct glycans, and compounds or vehicle controls were added. All compounds caused sperm release from immobilized glycans, demonstrating that hyperactivation was sufficient to release sperm from oviduct cells and immobilized glycans. Pharmacological inhibition of the non-genomic progesterone receptor and CatSper diminished sperm release from oviduct glycans. Inhibition of the proteolytic activities of the ubiquitin–proteasome system (UPS), implicated in the regulation of sperm capacitation, diminished sperm release in response to all hyperactivation inducers. In summary, induction of sperm hyperactivation was sufficient to induce sperm release from immobilized oviduct glycans and release was dependent on CatSper and the UPS.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hyperactivation is sufficient to release porcine sperm from immobilized oviduct glycans

Sharif

Hickl

Juárez

et al. 2022

Sci Rep

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“…The bovine samples were used with the permission of the Hokubu Agricultural Technology Institute, Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries (Hokubu Institute) under the research project plan “Improvement of Fertility Assay for Japanese Black Bull Spermatozoa (2016–2021)”. Fresh ejaculates were obtained for the routine investigation of semen characteristics to evaluate male reproductive performance, as described previously, with minor modifications [ 3 , 4 , 16 ]. In brief, an artificial vagina was used to collect the ejaculates from 10 sire candidates (Japanese Black bulls, > 1-year-old), which were fed at the Hokubu Institute.…”

Section: Methodsmentioning

confidence: 99%

“…To capture movies of the motility patterns of spermatozoa [ 3 , 4 ], an aliquot (10 µl) of the diluted semen or a suspension (10 µl) of washed spermatozoa was placed in a chamber with a depth of 50 µm (Fujihira Industry, Tokyo, Japan) on a warmed stage (38.5ºC) of an upright microscope (Olympus Corporation, Tokyo, Japan). Microscopic movies of the spermatozoa were captured using a CMOS camera (BU238M, Toshiba Teli, Tokyo, Japan) with × 40 objective lenses at a frame rate of 100 Hz and were recorded as non-compressed movies (FCR-1, TechnoScope, Saitama, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, new criteria for assessing bovine sperm motility have emerged. We recently conducted studies to improve methods of evaluating sperm flagellum functions using new criteria for helical movement with three-dimensional (3D) rotation [ 3 ] and 3D full-type hyperactivation [ 4 , 5 ]. The results of these studies indicated that the precise evaluation of these 3D movements requires a detailed investigation of the motility patterns of each spermatozoon in a chamber with sufficient depth (50 µm) under a microscope with relatively high magnification (× 40 objective lens).…”

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Involvement of Ca<sup>2+</sup>-ATPase in suppressing the appearance of bovine helically motile spermatozoa with intense force prior to cryopreservation

Duritahala

Sakase

Harayama

2022

Journal of Reproduction and Development

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In cattle, cryopreserved spermatozoa are generally used for artificial insemination (AI). Many of these specimens exhibit helical movement, although the molecular mechanisms underlying this phenomenon remain unclear. This study aimed to characterize helically motile spermatozoa, investigate the involvement of Ca 2+ -ATPase in suppressing the appearance of these spermatozoa prior to cryopreservation, and examine the potential of helical movement as an index of sperm quality. In the cryopreserved semen, approximately 50% of spermatozoa were helically motile, whereas approximately 25% were planarly motile. The helically motile samples swam significantly faster than those with planar movement, in both non-viscous medium and viscous medium containing polyvinylpyrrolidone. In contrast, in non-cryopreserved semen, planarly motile spermatozoa outnumbered those that were helically motile. Fluorescence microscopy with Fluo-3/AM and propidium iodide showed that flagellar [Ca 2+ ] i was significantly higher in cryopreserved live spermatozoa than in non-cryopreserved live ones. The percentage of non-cryopreserved helically motile spermatozoa was approximately 25% after washing, and this increased significantly to approximately 50% after treatment with an inhibitor of sarcoplasmic reticulum Ca 2+ -ATPases (SERCAs), “thapsigargin.” Immunostaining showed the presence of SERCAs in sperm necks. Additionally, the percentages of cryopreserved helically motile spermatozoa showed large inter-bull differences and a significantly positive correlation with post-AI conception rates, indicating that helical movement has the potential to serve as a predictor of the fertilizing ability of these spermatozoa. These results suggest that SERCAs in the neck suppress the cytoplasmic Ca 2+ -dependent appearance of helically motile spermatozoa with intense force in semen prior to cryopreservation.

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Effects of digoxin on full-type hyperactivation in bovine ejaculated spermatozoa with relatively lower survivability for incubation with stimulators of cAMP signaling cascades

et al. 2020

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Identification of isoforms of calyculin A-sensitive protein phosphatases which suppress full-type hyperactivation in bull ejaculated spermatozoa

Cited by 7 publications

References 53 publications

Hyperactivation is sufficient to release porcine sperm from immobilized oviduct glycans

Hyperactivation is sufficient to release porcine sperm from immobilized oviduct glycans

Involvement of Ca<sup>2+</sup>-ATPase in suppressing the appearance of bovine helically motile spermatozoa with intense force prior to cryopreservation

Effects of digoxin on full-type hyperactivation in bovine ejaculated spermatozoa with relatively lower survivability for incubation with stimulators of cAMP signaling cascades

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