Identification of isocyclosporins by collision-induced dissociation of doubly protonated species

Baranova, Anna A.; Chistov, Alexey A.; Shuvalov, Maxim V.; Tyurin, Anton P.; Biryukov, Mikhail V.; Ivanov, Igor A.; Садыкова, В. С.; Кураков, А. В.; Sergeeva, Albina I.; Korshun, Vladimir A.; Alferova, Vera A.

doi:10.1016/j.talanta.2020.121930

Cited by 2 publications

(2 citation statements)

References 36 publications

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“…Note the NMR-spectroscopy-deduced CycA content in isoA (approximately 9%). No N → O peptidyl shift occurred in the [M + 2H] 2+ ions of CycA, as previously reported …”

Section: Resultsmentioning

confidence: 99%

“…The N → O peptidyl shift has been shown to be suppressed in B/E sector and time-of-flight (TOF) instruments operating at a microsecond time frame . Since rearrangement in singly protonated species can make it difficult to distinguish CycA from isoA, collision-induced dissociation (CID) of doubly protonated species and metal complexation have been used to differentiate these isomers . Remarkably, the discussed rearrangement has not been mentioned in any previous ion mobility-mass spectrometry (IM-MS) study of CycA. − …”

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confidence: 99%

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Exploring the Effects of Cyclosporin A to Isocyclosporin A Rearrangement on Ion Mobility Separation

Mácha,

Zápal,

Kuzma

et al. 2024

Anal. Chem.

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Cyclosporin A (CycA) is a peptide secondary metabolite derived from fungi that plays a crucial role in transplantation surgery. Cyclic traveling wave ion mobility mass spectrometry (IM-MS) revealed an N → O peptidyl shift in singly protonated CycA to isocyclosporin A (isoA), whereas no such isomerization was observed for doubly protonated and sodiated molecules. CycA and isoA were able to be separated by considering doubly protonated precursors using a specific ion fragment. In

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“…Note the NMR-spectroscopy-deduced CycA content in isoA (approximately 9%). No N → O peptidyl shift occurred in the [M + 2H] 2+ ions of CycA, as previously reported …”

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Exploring the Effects of Cyclosporin A to Isocyclosporin A Rearrangement on Ion Mobility Separation

Mácha,

Zápal,

Kuzma

et al. 2024

Anal. Chem.

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Valorization of Cheese Whey as a Feedstock for Production of Cyclosporin A by Tolypocladium inflatum

et al. 2022

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Food waste-based biorefineries are considered an essential concept for the implementation of a sustainable circular economy. In this study, cheese whey powder (CWP), a dairy industry waste, was utilized to produce cyclosporin A (CsA). As it is difficult to valorize CWP because its components vary depending on the origin, a process for sugar conversion via acid hydrolysis was designed to obtain reproducible results using refined whey powder (WP) of a consistent quality. Acid hydrolysis was carried out using 2% (w/w) HCl and biomass loading of 50 g/L at 121 °C for 20 min. CWP hydrolysates were utilized to ferment Tolypocladium inflatum ATCC 34921. CsA production was found to be 51.3 mg/L at 12 days, a 1.4-fold increase compared to the control (commercial glucose, 36.3 mg/L). Our results showed that 100 g CWP can be converted to 81.8 mg of CsA. This finding demonstrated that CWP can be used as a sustainable feedstock for biorefineries.

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Identification of isocyclosporins by collision-induced dissociation of doubly protonated species

Cited by 2 publications

References 36 publications

Exploring the Effects of Cyclosporin A to Isocyclosporin A Rearrangement on Ion Mobility Separation

Exploring the Effects of Cyclosporin A to Isocyclosporin A Rearrangement on Ion Mobility Separation

Valorization of Cheese Whey as a Feedstock for Production of Cyclosporin A by Tolypocladium inflatum

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