Identification of Irradiated Prawn (Penaeus monodon) Using Thermoluminescence and 2-Alkylcyclobutanone Analyses

Abstract

Thermoluminescence (TL) and 2-alkylcyclobutanone (2-ACB) analyses were performed to identify irradiated prawns ( Penaeus monodon ). With the TL method, minerals were extracted from prawns using acid hydrolysis. The experimental results satisfied the evaluation criteria of European Norm (EN) 1788, even after low-dose irradiation (0.5 kGy) and a 60 day storage at -20 °C. With the 2-ACB method, 2-dodecylcyclobutanone (2-DCB) and 2-tetradecylcyclobutanone (2-TCB) were successfully extracted from prawns by direct solvent extraction with purification using a conventional silica column and a sulfoxide column, which was used for 2-ACB for the first time. Both 2-ACB derivatives were absent from the non-irradiated samples but were identified in all irradiated samples by gas chromatography-mass spectrometry. Moreover, 2-DCB and 2-TCB production correlated with the applied dose (2.5-10 kGy), and the correlation did not diminish after 60 days of storage at -20 °C for any dose. Therefore, these two techniques provide rapid, simple, and promising methods for routine investigation of frozen prawns.

Analysis of 2-alkylcyclobutanones for detection of food irradiation: Current status, needs and prospects

“…The extraction efficiency of hexane applied to prawns by shaking was studied by Chen et al (2011) who found that three sequential extractions were required for optimum recovery.…”

“…Further clean-up has been achieved by using a Supelclean TM sulfoxide column following a silica column for the determination of 2-ACBs in irradiated prawns (Chen et al, 2011). 2-ACBs were selectively eluted from the sulfoxide column and impurities removed from the GCMS chromatogram.…”

Modifications of azoxymethane-induced carcinogenesis and 90-day oral toxicities of 2-tetradecylcyclobutanone as a radiolytic product of stearic acid in F344 rats

“…At the end of the 90-day oral toxicity test, retroperitoneal adipose tissue was removed from 6 males and 3 females during autopsy, weighed and then stored at −80°C until use. Briefly, adipose tissue was homogenized with anhydrous sodium sulfate and extracted with n -hexane in a Soxhlet apparatus for 6 h. Concentrations of 2-tDCB in hexane extracts were analyzed using a GC-MS system (QP2010 Plus, Shimadzu, Kyoto, Japan) as per a previously described method 17 .…”

Estimation of Irradiation Doses of Raw Beef Liver Samples using 5,6-Dihydrothymidine as an Irradiation Marker

“…For example, the test solutions may have to be concentrated. 11 DNA is universally contained in the low-fat liver and lean meats, and its content would not be affected by the difference of individual of animals. Therefore, we have developed and presented a new method for determining beef liver irradiation histories by detecting of the sum of (5 S )- and (5 R )-5,6-dihydrothymidine (total DHdThd) as irradiation-induced modified nucleosides in DNA.…”