Identification of Iron-Responsive, Differential Gene Expression in the Cyanobacterium<i>Synechocystis</i>sp. Strain PCC 6803 with a Customized Amplification Library

Singh, Abhay K.; Sherman, Louis A.

doi:10.1128/jb.182.12.3536-3543.2000

Cited by 47 publications

(48 citation statements)

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“…These results are consistent with a dicistronic operon for nblA1 and nblA2 expression. A smear of transcripts has also been observed in the case of Synechocystis strain PCC 6803 nblA induced under iron starvation (27) and in the case of Synechococcus strain PCC 7942 nblA induced under S starvation (6).…”

Section: Resultsmentioning

confidence: 80%

Nitrogen or Sulfur Starvation Differentially Affects Phycobilisome Degradation and Expression of the nblA Gene in Synechocystis Strain PCC 6803

et al. 2001

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Nitrogen (N) limitation in cyanobacteria is well documented: a reduced growth rate is observed, accompanied by a cessation of phycobiliprotein synthesis and an ordered degradation of phycobilisomes (PBS). This leads to a dramatic bleaching phenomenon known as chlorosis. In Synechococcus strain PCC 7942, bleaching due to PBS degradation is also observed under sulfur (S) or phosphorus (P) limitation, and all three are under the control of the nblA gene product, a 59-amino-acid polypeptide which is overexpressed under N, S, and P starvation (J. L. Collier, and A. R. Grossman, EMBO J. 13:1039-1047, 1994). Cyanobase sequence data for Synechocystis strain PCC 6803 indicate the presence of two tandem open reading frames (sll0452 and sll0453) homologous to nblA. We cloned the two genes, identified a unique 5 mRNA end suggestive of a single transcription start site, and studied nblA expression under conditions of N or S starvation by Northern hybridization: transcripts were detected only under N starvation (no signal is detected in replete medium or with S starvation), whether nblA1 or nblA2 was used as a probe. Mutations in nblA1 and nblA2 were constructed by insertion of a kanamycin cassette; both mutations were nonbleaching under N starvation. Synechocystis strain PCC 6803 does not bleach under S starvation, consistent with the absence of nblA induction in these conditions. These results were confirmed by analysis of the PBS components: sequential degradation of phycocyanin and associated linkers was observed only under conditions of N starvation. This indicates differences between Synechocystis strain PCC 6803 and Synechococcus strain PCC 7942 in their regulatory and signaling pathways leading to N-and S-starved phenotypes.

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Section: Resultsmentioning

confidence: 80%

Nitrogen or Sulfur Starvation Differentially Affects Phycobilisome Degradation and Expression of the nblA Gene in Synechocystis Strain PCC 6803

et al. 2001

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“…strain PCC 6301. Therefore, the coordinated transcription of these genes in Synechocystis was not unexpected (6,58).…”

Section: Resultsmentioning

confidence: 99%

Genome-Wide Dynamic Transcriptional Profiling of the Light-to-Dark Transition inSynechocystissp. Strain PCC 6803

et al. 2002

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“…Starvation for phosphorus (Collier & Grossman, 1992), inorganic carbon (BarkerAstrom et al, 2005) or iron (Singh & Sherman, 2000) results in partial loss of pigment. Nitrogen and sulfur starvation, on the other hand, elicit substantial pigment loss in Synechococcus, with nitrogen depletion inducing rapid and complete phycobilisome degradation (Collier & Grossman, 1992).…”

Section: Degradation Of the Phycobilisomementioning

confidence: 99%

Acclimation of unicellular cyanobacteria to macronutrient deficiency: emergence of a complex network of cellular responses

2005

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Cyanobacteria are equipped with numerous mechanisms that allow them to survive under conditions of nutrient starvation, some of which are unique to these organisms. This review surveys the molecular mechanisms underlying acclimation responses to nitrogen and phosphorus deprivation, with an emphasis on non-diazotrophic freshwater cyanobacteria. As documented for other micro-organisms, nutrient limitation of cyanobacteria elicits both general and specific responses. The general responses occur under any starvation condition and are the result of the stresses imposed by arrested anabolism. In contrast, the specific responses are acclimation processes that occur as a result of limitation for a particular nutrient; they lead to modification of metabolic and physiological routes to compensate for the restriction. First, the general acclimation processes are discussed, with an emphasis on modifications of the photosynthetic apparatus. The molecular mechanisms underlying specific responses to phosphorus and nitrogen-limitation are then outlined, and finally the cross-talk between pathways modulating specific and general responses is described.

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Identification of Iron-Responsive, Differential Gene Expression in the CyanobacteriumSynechocystissp. Strain PCC 6803 with a Customized Amplification Library

Cited by 47 publications

References 46 publications

Nitrogen or Sulfur Starvation Differentially Affects Phycobilisome Degradation and Expression of the nblA Gene in Synechocystis Strain PCC 6803

Nitrogen or Sulfur Starvation Differentially Affects Phycobilisome Degradation and Expression of the nblA Gene in Synechocystis Strain PCC 6803

Genome-Wide Dynamic Transcriptional Profiling of the Light-to-Dark Transition inSynechocystissp. Strain PCC 6803

Acclimation of unicellular cyanobacteria to macronutrient deficiency: emergence of a complex network of cellular responses

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