Identification of international circulating lineages of meticillin-resistant Staphylococcus aureus in the north of Spain and their glycopeptide and linezolid susceptibility

Torres-Sangiao, Eva; Pérez-Castro, Sonia; Fernández-Natal, María Isabel; Cisterna-Cáncer, Ramón; Zapico-González, Marisol; Fernández-Pérez, Begoña; Ojeda-Fernández, Eva; Nebreda, Teresa; Gozalo-Margüello, Mónica; Fuster-Foz, Carlos; Roiz-Mesones, Maria Pía; Miguel-Martín, Maria Dolores; Torroba, L; Coira-Nieto, Amparo; Vasallo-Vidal, Francisco José; Méndez-Lage, Susana; Prieto-Rodriguez, Elisabeth; Eirós, José María; Torres, Julio; Bou, Germán

doi:10.1099/jmm.0.036889-0

Cited by 5 publications

(4 citation statements)

References 9 publications

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“…Pulsotype V belonged to ST2102 and this pulsotype was found in one farm. This ST has been reported in a Spanish hospital (Torres-Sangiao et al 2012); it belongs to the CC30, a dominant lineage in community and hospital associated S. aureus infections. It is reasonable to hypothesise that the detection of this ST was due to human contamination as we did not find any reports in the literature on the involvement of this ST in bovine mastitis.…”

Section: Resultsmentioning

confidence: 63%

Characterisation ofStaphylococcus aureusstrains isolated from mastitis bovine milk in Argentina

Srednik

Usongo

Lépine

et al. 2018

Journal of Dairy Research

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The study reported in this Research Communication was conducted to characterise Staphylococcus aureus isolates recovered from mastitic bovine milk from dairy herds in Argentina. A total of 829 mastitic milk samples, both clinical and subclinical, were collected from 21 farms by veterinarians and submitted to the laboratory for testing from which 229 S. aureus isolates were recovered, an isolation rate of 28·1%. These isolates were tested for susceptibility to the antibiotics penicillin, erythromycin and clindamycin. Of the 229 isolates, 53 (23·1%) were resistant to penicillin, 31 (13·5%) to erythromycin and 28 (12·2%) to clindamycin. All isolates were negative for the mecA, mecC and pvl genes by PCR. Southernblot hybridisation revealed that the ermC gene was located on plasmid bands. Eighty isolates were randomly selected from the 229 for further characterisation. Restriction analysis of chromosomal DNA with Cf9I followed by PFGE of the 80 isolates revealed 23 distinct pulsotypes at 80% similarity. Seven major types (A, B, N, P, S, T, U and V) accounted for 68·7% of these isolates and 12 pulsotypes (A, B, F, G, J, K, M, N, P, S, T and U) occurred on more than one farm indicating genetic diversity within the farms. MLST of a representative isolate from dominant types identified the STs 97 705, 746, 2102 and 2187 with ST97 being the most predominant. Antibiotic susceptibility testing showed that 53·7% of the 80 randomly selected isolates were resistant to at least one of the three antibiotics tested. To our knowledge, this study represents the first large scale molecular studies on S. aureus isolates from dairy farms in Argentina.

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Section: Resultsmentioning

confidence: 63%

Characterisation ofStaphylococcus aureusstrains isolated from mastitis bovine milk in Argentina

Srednik

Usongo

Lépine

et al. 2018

Journal of Dairy Research

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“…10 The clonal distribution of MRSA in our hospital differs from that found in the rest of Spain since the major HA-MRSA clones were pandemic, 43% ST5-MRSA-IV (Pediatric clone CC5), 39% ST22-MRSA-IV (EMRSA-15 CC22), and 14% ST36-MRSA-II (EMRSA-16 CC30), the latter two were not widespread in Spain except in Galicia and Mallorca. 26,35 In addition, clone ST125-MRSA-IV was scarcely present (0.6%) in our hospital 1,26,35,37 and among the CA-MRSA clones, the presence of clone ST72-MRSA-IV (USA 700), belonging to a recombination between CC5 and CC8, was also low (0.7%). 17 We observed the presence of clone ST398-MRSA-IV (1.3%) which, although scarce, reflects the introduction of LA-MRSA clones in our hospital, and the clone ST2574-MRSA-XI, which was recently identified in England, a mecC gene carrier of uncertain origin but possibly related to LA-MRSA clones.…”

Section: Discussionmentioning

confidence: 76%

Antimicrobial Resistance and Molecular Analysis of Methicillin-ResistantStaphylococcus aureusCollected in a Spanish Hospital

Hernández-Porto

Lecuona

Aguirre‐Jaime

et al. 2015

Microbial Drug Resistance

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Clonal distribution of methicillin-resistant Staphylococcus aureus (MRSA) in hospitals may differ according to the geographic location and time period. Knowledge of MRSA clonal epidemiology in hospital settings involves much more than the study of healthcare-associated MRSA (HA-MRSA) clones. In recent years, investigators have documented the introduction of both community-associated MRSA (CA-MRSA) and livestock-associated MRSA (LA-MRSA) clones, the emergence of clones carrying Staphylococcal cassette chromosome mec (SCCmec) XI, and the genetic diversity among sporadic MRSA isolates. The allocation of certain antibiotypes to dominant MRSA clones in an institution allows their use as phenotypic markers for a preliminary search for new clones, early detection of clonal shift, and as a guide for better empirical therapy, infection control, and treatment within a particular institution. For these reasons, we identified 938 strains detected in a System of Universal Active Surveillance of MRSA in clinical samples during the period 2009-2010, obtaining the clonal distribution of MRSA at the Hospital Universitario de Canarias (Tenerife, Spain) and the relationship between antimicrobial susceptibility and three major clones present. The antibiotypes that best defined the ST5-MRSA-IV (Pediatric) clone showed resistance to tobramycin and susceptibility to clindamycin, erythromycin, gentamicin, rifampin, trimethoprim-sulfamethoxazole, vancomycin, quinupristin/dalfopristin, and linezolid, whereas the ST22-MRSA-IV clone (EMRSA-15) showed susceptibility to these antibiotics, and finally, the ST36-MRSA-II clone (EMRSA-16) was resistant to clindamycin, erythromycin, and tobramycin and susceptible to the remaining antimicrobials. Similar observations would allow the early detection of changes in clonal epidemiology by analysis of antimicrobial susceptibility of the isolates within a single institution.

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“…8,10,19,22,43 Furthermore, EMRSA-15 has also been reported in other non-European countries, such as USA, 15 India, 14 and Malaysia; 20 however, in these locations it was not the predominant clone. EMRSA-15 has been recently reported to be found in high rates also in community settings in Portugal, suggesting the spillover of the clone from nosocomial settings into the community.…”

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confidence: 85%

Massive Dissemination of Methicillin ResistantStaphylococcus aureusin Bloodstream Infections in a High MRSA Prevalence Country: Establishment and Diversification of EMRSA-15

Faria

Miragaia

Lencastre

2013

Microbial Drug Resistance

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Portugal is the European country with the highest prevalence of methicillin resistant Staphylococcus aureus (MRSA), in which EMRSA-15 (ST22-IVh) has been the dominant clone since soon after its introduction in Portuguese hospitals in 2001. In this study, we intend to not only, assess the evolution of the invasive MRSA in Portuguese hospitals, but also to evaluate the invasive methicillin susceptible S. aureus (MSSA) population and the relationship between both populations. In the current study, two major MRSA clones were identified: EMRSA-15 that has been dominant for more than 10 years and accounts for 75% of the MRSA isolates, and ST105-II, a clone related with the New York/Japan clone (ST5-II). In contrast, among MSSA, several clonal backgrounds were identified. Despite of the massive predominance of EMRSA-15 in the last decade, an increase in spa diversity has been observed in the last few years, which suggests a recent and local diversification of this clone. Interestingly, MRSA and MSSA populations with related clonal backgrounds appear to have increased as a result of the dissemination of MRSA to the community environment.

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Identification of international circulating lineages of meticillin-resistant Staphylococcus aureus in the north of Spain and their glycopeptide and linezolid susceptibility

Cited by 5 publications

References 9 publications

Characterisation ofStaphylococcus aureusstrains isolated from mastitis bovine milk in Argentina

Characterisation ofStaphylococcus aureusstrains isolated from mastitis bovine milk in Argentina

Antimicrobial Resistance and Molecular Analysis of Methicillin-ResistantStaphylococcus aureusCollected in a Spanish Hospital

Massive Dissemination of Methicillin ResistantStaphylococcus aureusin Bloodstream Infections in a High MRSA Prevalence Country: Establishment and Diversification of EMRSA-15

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