Identification of interferon-γ-inducible-lysosomal thiol reductase (GILT) gene from Mefugu (Takifugu obscures) and its immune response to LPS challenge

Liu, Meng; Ai, Hongxin; Xiao, Wen; Shen, Yuefen; Shen, Yang; Cui, Xianwei; Zhang, Shuangquan

doi:10.1016/j.dci.2013.04.023

Cited by 17 publications

(2 citation statements)

References 33 publications

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“…In mammals, the gamma-interferon-inducible lysosomal thiol reductase (GILT) is involved in the processing and presentation of antigens into the major histocompatibility complex (MHC) class II [13]. Similar functions have also been described for several orthologous fish genes [14][15][16][17][18][19][20][21]. Furthermore, it has been described that GILT controls the replication of murine leukaemia virus and human immunodeficiency virus type 1 [22,23]; therefore, the gilt downregulation detected in wt160-infected animals could contribute to increase viral virulence.…”

Section: Discussionmentioning

confidence: 80%

Immune Response of Senegalese Sole against Betanodavirus Mutants with Modified Virulence

et al. 2021

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Nervous necrosis virus (NNV), genus Betanodavirus, the etiological agent of the viral encephalopathy and retinopathy (VER), presents a genome with two positive-sense single-stranded RNA segments. Striped jack nervous necrosis virus (SJNNV) and red-spotted grouper nervous necrosis virus (RGNNV), together with reassortants RGNNV/SJNNV, are the betanodaviruses predominantly isolated in Southern Europe. An RGNNV/SJNNV reassortant isolated from Senegalese sole (wt160) causes high mortalities in this fish species. This virus presents differences in the sequence of the 3’ non-coding region (NCR) of both segments compared to RGNNV and SJNNV reference strains. Previously, it has been reported that the reversion of two of these differences (nucleotides 1408 and 1412) in the RNA2 3’NCR to the SJNNV-type (recombinant r1408-1412) resulted in a decrease in sole mortality. In the present study, we have applied an OpenArray® to analyse the involvement of sole immune response in the virulence of several recombinants: the r1408-1412 and two recombinants, developed in the present study, harbouring mutations at positions 3073 and 3093 of RNA1 3’NCR to revert them to RGNNV-type. According to the correlation values and to the number of expressed genes, the infection with the RNA2-mutant provoked the most different immune response compared to the immune response triggered after the infection with the rest of the viruses, and the exclusive and high upregulation of genes related to the complement system. The infection with the RNA1-mutants also provoked a decrease in mortality and their replication was delayed at least 24 h compared to the wt160 replication, which could provoke the lag observed in the immune response. Furthermore, the infection with the RNA1-mutants provoked the exclusive expression of pkr and the downregulation of il17rc.

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Section: Discussionmentioning

confidence: 80%

Immune Response of Senegalese Sole against Betanodavirus Mutants with Modified Virulence

et al. 2021

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“…Recently, GILT genes have been cloned and characterized in many fishes such as large yellow croaker [20], orange-spotted grouper [21], zebrafish [22], Mefugu [23] and rainbow trout [24]. In order to know more about fish GILT family genes, the cDNA sequence of GILT from mandarin fish was determined by experimental method.…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning, expression and functional analysis of ISG15 in orange-spotted grouper, Epinephelus coioides

Huang

Cai

et al. 2013

Fish & Shellfish Immunology

104

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Characterization and expression analysis of the complement component 8β from pufferfish (Takifugu rubripes)

Xia

Hua

Zhu

et al. 2021

Aquaculture Reports

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Identification of interferon-γ-inducible-lysosomal thiol reductase (GILT) gene from Mefugu (Takifugu obscures) and its immune response to LPS challenge

Cited by 17 publications

References 33 publications

Immune Response of Senegalese Sole against Betanodavirus Mutants with Modified Virulence

Immune Response of Senegalese Sole against Betanodavirus Mutants with Modified Virulence

Molecular cloning, expression and functional analysis of ISG15 in orange-spotted grouper, Epinephelus coioides

Characterization and expression analysis of the complement component 8β from pufferfish (Takifugu rubripes)

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