Identification of insulin-like peptides in cerebral ganglia neurosecretory cells of the mussel Mythus edulis

Kellner-Cousin, Kristell; Mialhe, Eric; Mathieu, Michel

doi:10.1016/0040-8166(94)90038-8

Cited by 25 publications

(10 citation statements)

References 38 publications

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“…For example, the insulin-related peptide was identified in Mytilus edulis [5], Crassostrea gigas [6] and Anodonta cygnea [7], and the insulin receptor-related receptor that showed an association with insulin-like effects on growth was found in C . gigas [8].…”

Section: Introductionmentioning

confidence: 99%

A Scallop IGF Binding Protein Gene: Molecular Characterization and Association of Variants with Growth Traits

Feng

et al. 2014

PLoS ONE

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BackgroundScallops represent economically important aquaculture shellfish. The identification of genes and genetic variants related to scallop growth could benefit high-yielding scallop breeding. The insulin-like growth factor (IGF) system is essential for growth and development, with IGF binding proteins (IGFBPs) serving as the major regulators of IGF actions. Although an effect of IGF on growth was detected in bivalve, IGFBP has not been reported, and members of the IGF system have not been characterized in scallop.ResultsWe cloned and characterized an IGFBP (PyIGFBP) gene from the aquaculture bivalve species, Yesso scallop (Patinopecten yessoensis, Jay, 1857). Its full-length cDNA sequence was 1,445 bp, with an open reading frame of 378 bp, encoding 125 amino acids, and its genomic sequence was 10,193 bp, consisting of three exons and two introns. The amino acid sequence exhibited the characteristics of IGFBPs, including multiple cysteine residues and relatively conserved motifs in the N-terminal and C-terminal domains. Expression analysis indicated that PyIGFBP was expressed in all the tissues and developmental stages examined, with a significantly higher level in the mantle than in other tissues and a significantly higher level in gastrulae and trochophore larvae than in other stages. Furthermore, three single nucleotide polymorphisms (SNPs) were identified in this gene. SNP c.1054A>G was significantly associated with both shell and soft body traits in two populations, with the highest trait values in GG type scallops and lowest in AG type ones.ConclusionWe cloned and characterized an IGFBP gene in a bivalve, and this report also represents the first characterizing an IGF system gene in scallops. A SNP associated with scallop growth for both the shell and soft body was identified in this gene. In addition to providing a candidate marker for scallop breeding, our results also suggest the role of PyIGFBP in scallop growth.

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Section: Introductionmentioning

confidence: 99%

A Scallop IGF Binding Protein Gene: Molecular Characterization and Association of Variants with Growth Traits

Feng

et al. 2014

PLoS ONE

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“…Although oIRP cDNA was identified in VG of Pacific oyster, the cerebral ganglia (CG) have been suggested to produce IRP in M. edulis and M. galloprovincialis (Kellner-Cousin et al 1994, Danton et al 1996. In addition, insulin-like immunoreactivity has been reported to localize to the digestive tract of some bivalves using an antibody raised against mammalian insulin (Fritsch et al 1976, Plisetskaya et al 1978.…”

Section: An Insulin-related Peptide In the Pacific Oyster · K Hamano mentioning

confidence: 99%

“…Cells within the intestinal epithelia of Ostrea edulis (Martinez et al 1973), Mytilus edulis (Fritsch et al 1976), Anodonta cygnea and Unio pictorum (Plisetskaya et al 1978), and neurosecretory cells in the cerebral ganglia of M. edulis (Kellner-Cousin et al 1994) have stained positive for mammalian insulin by immunohistochemistry. In M. galloprovincialis, anti-Anodonta insulin immunoreactivity was observed in the cerebral ganglia, and the annual cycle of the immunoreactivity in relation to the physiological changes was examined (Danton et al 1996).…”

Section: Introductionmentioning

confidence: 99%

cDNA structure of an insulin-related peptide in the Pacific oyster and seasonal changes in the gene expression

Hamano¹,

Awaji²,

Usuki³

2005

Journal of Endocrinology

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Insulin-related peptide cDNA was characterized in the Pacific oyster Crassostrea gigas. It was determined that three transcripts with differing lengths of 3 -untranslated region (3 -UTR) were expressed in the visceral ganglia. The insulin-related peptide cDNA contained a number of AUUUA motifs that were typical of adenylate/uridylaterich elements in the 3 -UTR. The deduced preprohormone was a polypeptide of 161 residues and showed a conformation typical of preprohormones of the insulin superfamily, which included conserved amino acids necessary to adopt the globular insulin structure. The expression of the three different transcripts was variable throughout the year, with the highest expression observed in March and lower expression in November and July.

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“…It is also possible that some or all of these substances are only insulinlike because they share with human insulin the antigenic site recognized by the antibody. Using polyclonal antibody to the C-peptide of co-workers (see Smit et al 1998), who found that L. stagnalis MIP and gel-filtration chromatography, Kellner-Cousin et al (1994) also detected more than one fraction with insulin-like material in the CG extract of the bivalve M. edulis.…”

Section: Discussionmentioning

confidence: 93%

Localization and immunological characterization of insulin-like peptide(s) in the land snail Otala lactea (Mollusca: Pulmonata)

Abdraba¹,

Saleuddin²

2000

Can. J. Zool.

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Insulin-like peptides were detected by means of immunological techniques in tissues of the land snail Otala lactea. Insulin-positive cells were detected in all the ganglia except the right parietal ganglion and visceral ganglion. In the digestive tract, insulin-positive cells were found in the muscle and connective tissue layer of the intestine. The amount of insulin-like peptide detected in acid-ethanol extract of brains and digestive tracts from active snails did not differ significantly from that in the corresponding tissues from estivating (dormant) ones. More insulin-like peptide was detected in hemolymph from active snails than in hemolymph from estivating ones. Brains from active snails released insulin-like peptide in vitro. Analysis of the cerebral ganglia or digestive tract extracts by size-exclusion chromatography and insulin RIA revealed more than one fraction with insulin immunoreactivity. Some of these fractions contained material with molecular masses close to those of mammalian insulin or its subunits. Further analysis of the extracts by reverse-phase chromatography also revealed more than one fraction with immunoreactivity. The immunoreactive material from the digestive tract was found to be less hydrophobic than insulin. Western blot analysis of the cerebral ganglia extract revealed more than one band with insulin immunoreactivity. Three of these bands had molecular masses very similar to those of vertebrate insulin, its subunits, and its precursor.

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Identification of insulin-like peptides in cerebral ganglia neurosecretory cells of the mussel Mythus edulis

Cited by 25 publications

References 38 publications

A Scallop IGF Binding Protein Gene: Molecular Characterization and Association of Variants with Growth Traits

A Scallop IGF Binding Protein Gene: Molecular Characterization and Association of Variants with Growth Traits

cDNA structure of an insulin-related peptide in the Pacific oyster and seasonal changes in the gene expression

Localization and immunological characterization of insulin-like peptide(s) in the land snail Otala lactea (Mollusca: Pulmonata)

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