Identification of insulin-like growth factor-I in boar seminal plasma and its influence on sperm quality

Zangerônimo, Márcio Gilberto; Silva, D.M.; Murgas, Luís David Solis; Sousa, Raimundo Vicente de; Rocha, Luiz Célio Souza; Pereira, Bárbara Azevedo; Faria, Bruno Generoso; Veras, Galileu Crovatto

doi:10.4321/s0004-05922013000300009

Cited by 8 publications

(7 citation statements)

References 14 publications

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“…In accordance with current results similar concentrations of IGF-1 were reported in the seminal plasma of buffalo bulls by Kumar et al (2019). Zangeronimo et al (2013) reported a relatively low range of free IGF-1 concentration in boar seminal plasma, which is significantly lower compared with the findings of our study. IGF-1 in seminal plasma is primarily derived from the testes or epididymis (Glander et al, 1996) and synthesized by Sertoli cells and Leydig cells under the influence of FSH and LH, respectively (Lejeune et al, 1996).…”

Section: Discussionsupporting

confidence: 93%

Association between levels of insulin‐like growth factor‐1 in serum and seminal plasma with fresh and frozen‐thawed semen characteristics in Beetal bucks

Kumar,

Dutta,

Deori

et al. 2023

Reprod Domestic Animals

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The aim of this study was to investigate the relationship between the levels of insulin‐like growth factor‐1 (IGF‐1) in serum and seminal plasma and the characteristics of semen in Beetal bucks (Capra hircus). A total of 12 adult Beetal bucks were involved in the study, with each buck providing six ejaculates collected using a standard artificial vagina (n = 72 total). Only qualified semen samples (volume of 0.7 mL, a mass motility rating of 3+ or higher on a 0–+ scale, and individual progressive motility of 80% or more) divided into three fractions were processed for estimation of IGF‐1 and other seminal parameters like motility, viability, acrosome integrity, sperm abnormality and superoxide dismutase (SOD) activity. The first and second fraction were diluted and extended with Optixcell extender (1:15 ratio). The first ejaculate fraction was processed for studying fresh semen parameters and the second fraction was cryopreserved for evaluating frozen semen parameters. French mini straws (0.25 mL) were used for semen filling, and polyvinyl alcohol powder of different colours was used for sealing the extended semen. The third fraction of each ejaculate was centrifuged at room temperature (1100 × g for 7 min) to separate the seminal plasma. Additionally, blood samples were taken from each buck on the same day as semen collection, resulting in a total of 36 blood samples. The results revealed a significant positive correlation (r = .4243; p < .05) between the concentration of IGF‐1 in both serum and seminal plasma of the Beetal bucks. Furthermore, the concentration of IGF‐1 in serum showed significant positive correlations with sperm viability (r = .554; p < .05), acrosome integrity (r = .527; p < .05), post‐thaw sperm motility (r = .407; p < .01), post‐thaw sperm viability (r = .426; p < .01) and post‐thaw acrosome integrity (r = .333; p < .05). However, it had a significant negative correlation with SOD activity in fresh semen (r = −0.458; p < .01). Moreover, the concentration of IGF‐1 in seminal plasma demonstrated significant positive correlations with individual progressive motility (r = .341; p < .05), sperm viability (r = .527; p < .05), acrosome integrity (r = .539; p < .05), sperm plasma membrane integrity (r = .464; p < .05), post‐thaw sperm motility (r = .644; p < .01), post‐thaw sperm viability (r = .643; p < .01), post‐thaw acrosome integrity (r = .487; p < .01) and post‐thaw sperm plasma membrane integrity (r = .521; p < .01). Additionally, it showed a significant negative correlation with SOD activity in both fresh semen (r = −0.714; p < .01) and frozen semen (r = −0.558; p < .01) of Beetal bucks. Based on these findings, IGF‐1 in seminal plasma can be considered as a potential biomarker for the selection of bucks for breeding purposes.

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Section: Discussionsupporting

confidence: 93%

Association between levels of insulin‐like growth factor‐1 in serum and seminal plasma with fresh and frozen‐thawed semen characteristics in Beetal bucks

Kumar,

Dutta,

Deori

et al. 2023

Reprod Domestic Animals

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“…In men, the serum IGF-1 level was associated with normal sperm morphology and percentage of sperm motility [ 22 ]. It has been shown that the addition of IGF-1 into seminal plasma can improve sperm quality [ 21 , 23 , 24 , 25 ]. Furthermore, in the seminal plasma, IGF-1 has been investigated in boars [ 23 , 26 ], cattle [ 16 ], stallions [ 15 ] and humans [ 17 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

“…It has been shown that the addition of IGF-1 into seminal plasma can improve sperm quality [ 21 , 23 , 24 , 25 ]. Furthermore, in the seminal plasma, IGF-1 has been investigated in boars [ 23 , 26 ], cattle [ 16 ], stallions [ 15 ] and humans [ 17 , 22 ]. Seminal IGF-1 concentration appears to be related to greater semen quality [ 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Serum Insulin-like Growth Factor-1 Is a Biomarker of Testosterone Production and Intact Acrosome in Asian Elephants (Elephas maximus)

Yang

Suwimonteerabutr

Angkawanish

et al. 2022

Animals

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The objective of this study was to find relationships among serum IGF−1, serum testosterone, seminal plasma IGF−1 concentrations and semen parameters in Asian elephants (Elephas maximus). A total of 17 ejaculates (one to three ejaculates/bull) were collected from seven captive elephant bulls by performing rectal massage. Before each ejaculation, blood samples were obtained for serum IGF−1 and testosterone assays. Subsequently, the semen characteristics of each ejaculate were evaluated. Mean serum IGF−1 concentration of elephant bulls was estimated as 326.3 ± 114.6 ng/mL (median, 286.2 ng/mL; range, 167.4–542.7 ng/mL). An increase in serum IGF−1 concentration was found to correlate with the percentage of spermatozoa with intact acrosomes. In addition, IGF−1 concentration was positively correlated with testosterone level. However, seminal IGF−1 concentrations could not be detected. In conclusion, our findings suggest that serum IGF−1 concentration is likely a biomarker of normal testicular functions, particularly spermatogenesis in elephants. Moreover, this commercial IGF−1 ELISA is eligible for analyzing serum IGF−1 concentration in Asian elephants.

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“…IGF-1 receptors (IGF-1Rs) have been identified in the spermatogonia, spermatocytes, early spermatids, spermatozoa, Sertoli cells, and Leydig cells (Vannelli et al, 1988;Le Gac et al, 1996;Henricks et al, 1998;Naz and Padman, 1999; and cells of the epididymis (Leheup and Grigong, 1993;Yoon et al, 2015). In the seminal plasma, IGF-1 has been investigated in boar (Hirai et al, 2001;Zangeronimo et al, 2013), bovine (Henricks et al, 1998), equine , human (Naz and Padman, 1999;Lee et al, 2016), rabbit (Minelli et al, 2001), and fish (Le Gac et al, 1996). All the above evidence is supporting that IGF-1 is essential in signal-regulating spermatogenesis in mammals.…”

Section: Insulin-like Growth Factor-1 (Igf-1)mentioning

confidence: 99%

“…In boars, the addition of IGF-1 into seminal plasma (mean, 1.5 ± 0.20 ng/mL; range, 1.39 to 2.44 ng/mL) can maintain sperm longevity (Zangeronimo et al, 2013).…”

Section: Igf-1 and Male Infertilitymentioning

confidence: 99%

Relationship between insulin-like growth factor-1 and semen quality in Asian elephants (elephas maximus)

Yang¹

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Evidence has been increasingly suggested that insulin-like growth factor-1 (IGF-1) is related to steroidogenesis and spermatogenesis. This may be conducive to find the causes of poor sperm quality in Asian elephants (Elephas maximus). The present study aimed to investigate whether correlations among serum IGF-1 concentration, serum testosterone level and semen variables exist in elephants. A total of 17 ejaculates (1–3 ejaculates/bull) were collected by performing transrectal massage. Before each ejaculate, blood samples were obtained (n = 17). Subsequently, semen characteristics of each ejaculate were evaluated. Assessments of precision (calculations of the intra- and inter-assay coefficient of variation, CV) and accuracy (tests of linearity and spike-recovery) were performed for ELISA validation. An increase of serum IGF-1 concentration was found to correlate with the percentages of spermatozoa with intact acrosome (r = 0.53, P < 0.05) and normal head morphology (r = 0.48, P < 0.05). The serum IGF-1 concentration was positively correlated with serum testosterone level (r = 0.73, P = 0.004). The results of validation demonstrated the CV was 1.6–6.4% for intra-assay variability and 4.7–6.9% for inter-assay variability. Linearity under serial dilutions of a known serum concentration was confirmed (R2 = 0.99) with an acceptable recovery rate of each dilution (mean 114.7 ± 19%, ranging from 100 to 143%). Additionally, the mean percentage of recovery of spiked IGF-1 was 107.2 ± 21% (ranging from 80.8 to 136.9%). In summary, this commercial ELISA kit can be used to determine serum IGF-1 concentration in Asian elephants. Moreover, our findings suggest the increased IGF-1 may be relate to good sperm quality and that the relationship between serum IGF-1 and testosterone concentration indicates a crucial role in the fertility of male elephants. Further works are interesting to investigate the exact mechanism by which IGF-1 affects semen quality in elephants.

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Identification of insulin-like growth factor-I in boar seminal plasma and its influence on sperm quality

Cited by 8 publications

References 14 publications

Association between levels of insulin‐like growth factor‐1 in serum and seminal plasma with fresh and frozen‐thawed semen characteristics in Beetal bucks

Association between levels of insulin‐like growth factor‐1 in serum and seminal plasma with fresh and frozen‐thawed semen characteristics in Beetal bucks

Serum Insulin-like Growth Factor-1 Is a Biomarker of Testosterone Production and Intact Acrosome in Asian Elephants (Elephas maximus)

Relationship between insulin-like growth factor-1 and semen quality in Asian elephants (elephas maximus)

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