Identification of informative markers of agronomic traits in different ecotypes of sand plantain (Plantago psyllium)

Rahimi, Mehdi; Maleki, Hamid Hatami; Mortezavi, Mojtaba

doi:10.6001/biologija.v63i4.3607

Cited by 4 publications

(5 citation statements)

References 10 publications

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“…Out of a total of 126 amplified DNA bands, up to 66 polymorphic DNA bands accounted for 66.2% with an average of 6.6 polymorphic bands appearing on each primer. This finding is consistent with previous study of Rahimi and colleagues when using the same ISSR primer set and found the polymorphism of 69.91% in different ecotypes of sand plantain (Rahimi et al 2017). This data also demonstrates that the primers used in the study are capable of amplifying in highly genetically distinct regions between gac samples.…”

Section: Resultssupporting

confidence: 93%

Genetic characterization of Gac (Momordica cochinchinesis) accessions in Southern Vietnam by ISSR markers

Nguyen

2019

Biodiversitas

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Ho VT, Le HT, Nguyen TA. 2019. Genetic characterization of Gac (Momordica cochinchinesis) accessions in Southern Vietnam by ISSR markers. Biodiversitas 20: 387-392. Gac (Momordica cochinchinesis) plays important roles in Vietnamese cuisine and medicine. It has high nutrient and medicinal values. Demand of gac fruit as ingredient for food processing and raw material for health industry requires accelerated gac cultivation. However, gac quality is variable due to the uncertain available germplasm and its management as breeding stock. Identification of gac cultivars and landraces in Vietnam is mainly based on personal experience relying on morphological traits, thus the conservation and breeding have low impact on productivity and quality of product. Recently, the development of molecular markers has been useful in identifying specific plant(s). In this study, genetic richness and relativeness of 14 gac accessions collected from different provinces in Southern Vietnam were evaluated by using 10 Inter-Simple Sequence Repeats (ISSR) markers. Results revealed large variation in genetic background of studied gac accessions. Seven DNA-based markers with potential to differentiate gac genotypes were recorded. The results provide molecular biological information for classification, identification plant origins, breeding and conserving programs of gac in Vietnam.

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Section: Resultssupporting

confidence: 93%

Genetic characterization of Gac (Momordica cochinchinesis) accessions in Southern Vietnam by ISSR markers

Nguyen

2019

Biodiversitas

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“…The mean PIC obtained for the ISSR primers (=0. 2727) in the present study was lower than the mean PIC (=0.36) estimated for 12 ISSR primers in P. psyllium in the Rahimi et al[36] study. This result shows the ISSR primers tested in our study were informative with respect to discerning genetic diversity of the eight Plantago species.…”

contrasting

confidence: 79%

“…Presently, analysis of 25 ISSR primers resulted in above 80% polymorphism detected in the 31 Plantago accessions. In other studies with Plantago 19.35% (Kaswan et al[4] and 70.54%[36] polymorphisms were identified for ISSR primers tested in P. ovata and P. psyllium, respectively. Analysis of PIC analysis provides an estimate of the discriminating power of a marker.…”

mentioning

confidence: 84%

Analysis of genetic diversity and association of seed and mucilage yields with inter simple sequence repeats (ISSR) polymorphism in a collection ofPlantgaospecies

Bagheri

Heidari

Shahriari

et al. 2020

Preprint

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AbstractAnalysis of genetic diversity in medicinal plants assists germplasm conservation and selection for use in breeding schemes. The aims of the present study were to assess genetic diversity and differentiation of several Plantago species using Inter Simple Sequence Repeats (ISSR) markers and identify marker-trait associations (MTAs). Thirty-one Plantago accessions belonging to eight species with various mating system and chromosome number were collected from geographical regions of Iran environments. Polymorphism in the DNA of Plantago accessions were analyzed using polymerase chain reaction (PCR) of 25 ISSR primers. The data for number of polymorphic bands were analyzed on the basis of several genetic diversity parameters. The results of gel analysis indicated that the ISSR primers amplified 5 to 21 polymorphic bands with 100 to 3000 bp size. The mean polymorphism was 83.83% and five primers showed 100% polymorphism among Plantago accessions. The Polymorphism Information Content (PIC) for ISSR as a dominant marker ranged from 0.1103 to 0.3829 with the mean 0.2727 in the species tested. Accessions in P. amplexicaulis and P. pysillum species represented the highest Nei’s and Shannon’s genetic diversity whilst the lowest obtained for P. lagopus. Analysis of phylogenetic network generated by the Neighbor-Net Algorithm showed moderate split of the eight species tested and the network depicted moderate conflict. The principal coordinate analysis (PCoA) results showed lower conflict in separation of accessions of the eight species. Fifty-six significant MTAs were detected for the traits tested in Plantago accessions, of which six were shared between three seed and mucilage traits and 24 were common between two traits. The coefficient of determination (R2) for the identified MTAs varied between 32 and 73%. In conclusion, the results of genetic diversity analysis suggested that ISSR marker could efficiently differentiate Plantago species and the information of genetic diversity might assist Plantago improvement and conservation.

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“…The high value of the MI for ISSRs is the result of highlights the distinctive nature of these markers. The higher the MI, the better method is used (Rahimi et al, 2018).…”

Section: Genotypes Characterizationmentioning

confidence: 99%

Molecular Diversity of Walnut (Juglans regia L.) Among Two Major Areas in Morocco in Contrast with Foreign Varieties

Houmanat

Kajji

Hssaini

et al. 2020

International Journal of Fruit Science

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In Morocco, walnut tree (Juglans regia L.) cultivation is ancestral with complicated misidentification and unknown local genetic diversity. Atlas Mountains host a rich walnut tree germplasm, which has arisen from seedlings. The use of molecular markers makes it possible to overcomes confusion usually encountered in phenotypic characterization, thereby elucidating the real genetic diversity magnitude. This study aimed to investigate the genetic relationships among 33 local walnut trees grown in two contrasted agroecosystems, while comparing them with 8 Bulgarian varieties belonging to an ex-situ collection, using ISSR markers. The use of 13 primers generated 120 reproducible ISSR markers, with 7 to 13 bands and an average of nine bands per primer. This number reflected the high level of polymorphism between genotypes revealed by the selected primers. The average polymorphism rate was 75.2%, with the polymorphic information content varying from 0.212 to 0.370 and with an average of 0.32. Genetic distances and the pairwise comparison showed that all pairs of genotypes are distinct using more than 10 markers with a maximum of 50 bands. The dendrogram showed an obvious phylo-geographic structuring and the differentiation of genotypes by origin area. The Bulgarian cultivars form distinct groups that confirms the specificity of the Moroccan walnut gene pool. However, the dendrogram obtained showed that the Moroccan genotypes are genetically close to a certain number of Bulgarian genotypes. This indicates that some Moroccan genotypes and the Bulgarian varieties could have a common ancestor.

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Identification of informative markers of agronomic traits in different ecotypes of sand plantain (Plantago psyllium)

Cited by 4 publications

References 10 publications

Genetic characterization of Gac (Momordica cochinchinesis) accessions in Southern Vietnam by ISSR markers

Genetic characterization of Gac (Momordica cochinchinesis) accessions in Southern Vietnam by ISSR markers

Analysis of genetic diversity and association of seed and mucilage yields with inter simple sequence repeats (ISSR) polymorphism in a collection ofPlantgaospecies

Molecular Diversity of Walnut (Juglans regia L.) Among Two Major Areas in Morocco in Contrast with Foreign Varieties

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