Identification of infectious spleen and kidney necrosis virus from farmed barramundi Lates calcarifer in Thailand and study of its pathogenicity

Draft genome sequence of scale drop disease virus (SDDV) retrieved from metagenomic investigation of infected barramundi, Lates calcarifer (Bloch, 1790)

“…Nevertheless, interpretation of virus metagenomic analysis from fish sample should always be conducted very carefully because mortalities due to concurrent infections in cultured fish usually outweigh single infection (Dong et al., 2015). Although concurrent infection between distinct viral taxa has yet to be documented in farmed barramundi, the emergence of mortalities caused by numerous viral pathogens including megalocytiviruses (SDDV and ISKNV) and recently described LCBV in South‐East Asia indicated that the possibility of coinfection in the field should not be omitted from diagnostic process (de Groof et al., 2015; Chen et al., 2019; Dong, Jitrakorn, et al, 2017; Dong, Taengphu, et al, 2017; Senapin et al., 2019; Thanasaksiri, Takano, Fukuda, Chaweepack, & Wongtavatchai, 2019). In the case of complicated and emerging diseases, polyphasic approaches combining pathogen detection tools, for example virus isolation, qPCR and conventional assays, in situ hybridization, immunohistochemistry and host response investigation, for example histopathology and experimental challenge, are still indispensable in making a definitive diagnosis.…”

Isolation of infectious spleen and kidney necrosis virus from farmed Asian seabass in Indonesia and effect of fish size on its virulence

“…Based on these results, BPBAP/KS1801, BPBAP/KS1802, BPBAP/KS1803, BPBAP/KS1804, and BPBAP/KS1805 were found to belong to ISKNV genotype I. In addition, these results indicated ISKNV genotype I is a major cause of iridoviral disease in Asian seabass culture in ASEAN countries (Kurita & Nakajima, 2012; Thanasaksiri et al., 2019).…”

“…Infectious spleen kidney and necrosis virus infection was recently reported in Asian seabass in Thailand (Thanasaksiri et al, 2019) and…”

“…The tissue debris was used for total DNA extraction using DNeasy 96 Blood and Tissue Kit according to the manufacturer's protocol (Qiagen). After testing with RSIV/ISKNV-specific primers (Kurita, Nakajima, Hirono, & Aoki, 1998), all diseased fish were shown to be iridovirus positive (Kurita & Nakajima, 2012;Thanasaksiri et al, 2019).…”

Fermented Animal Source Protein as Substitution of Fishmeal on Intestinal Microbiota, Immune-Related Cytokines and Resistance to Vibrio mimicus in Freshwater Crayfish (Cherax cainii)

“…Aquaculture, one of the fastest growing food-producing sectors in the world, produced 80 million tonnes of edible fish valued at USD 231.6 billion in 2016, with crustacean aquaculture contributing 7.9 million tonnes with a value estimated at USD 57.1 billion (FAO, 2018). Mass production through intensive farming practices may possibly result in the prevalence of pathogens and subsequent disease infestation (Thanasaksiri et al, 2019). As a result, utilization of drugs and antibiotics to control pathogens and disease outbreaks is now common practice in aquaculture.…”