Identification of immune-related genes in gills of Chinese mitten crabs (Eriocheir sinensis) during adaptation to air exposure stress

Bao, Jie; Xing, Yuenan; Jiang, Hongbo; Li, Xiaodong

doi:10.1016/j.fsi.2018.10.085

Cited by 28 publications

(14 citation statements)

References 57 publications

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“…The experiment was divided into two stages: air exposure stress and reimmersion after stress. Previous experiments showed that Chinese mitten crabs (similar size in present study) may die when exposed to air for 20 h, but that they can survive normally after exposure to air for 16 h (Bao et al, 2019). The experiment included five treatment groups of different air exposure times (0, 2, 4, 8, and 16 h, with 0 h as the control).…”

Section: Methodssupporting

confidence: 63%

Respiratory Metabolism and Antioxidant Response in Chinese Mitten Crab Eriocheir sinensis During Air Exposure and Subsequent Reimmersion

Bao

Xing

et al. 2019

Front. Physiol.

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Chinese mitten crab, Eriocheir sinensis , often suffers from severe air exposure stress during transportation and culture; high mortality occurs due to desiccation. In this study, the effects of air exposure stress (0, 2, 4, 8, and 16 h) and reimmersion (2, 6, 12 h) on respiratory metabolism and antioxidant responses in Chinese mitten crabs were studied under laboratory conditions. The results showed that air exposure and reimmersion had a significant impact on the oxygen consumption rate (OCR), ammonia excretion rate (AER), oxygen to nitrogen ratio (O:N), superoxide dismutase (SOD), catalase (CAT), succinate dehydrogenase (SDH), and lactate dehydrogenase (LDH). Significant interaction between air exposure and reimmersion was observed for OCR, AER, O:N, SOD, CAT, SDH, and LDH in Chinese mitten crab. During the air exposure stage, SOD, CAT, and LDH activities in the gills and hepatopancreas first increased and then decreased as air exposure time increased. All of these parameters were significantly higher in the 4-h air exposure group than those in the control group. All the parameters were significantly lower in the 16-h air exposure group than those in the control group, except LDH in the hepatopancreas. However, SDH activity gradually decreased with increased air exposure time, and all the air exposure groups were markedly lower than those in the control group in the gills. During the reimmersion stage, OCR, AER, and O:N restored to normal levels after 12-h reimmersion, except in the 16-h air exposure group, where OCR and O:N were significantly higher than those in the control group and AER was significantly lower than that in the control group. The LDH activity in all groups restored to normal levels after 12-h reimmersion. The SDH, SOD, and CAT activities of the 2- and 4-h air-exposed groups returned to normal levels after 12-h reimmersion; however, these three parameters were still significantly higher in the 16-h air-exposed group than in the control group in the gills and hepatopancreas. Overall, Chinese mitten crabs reduce aerobic respiration and increase anaerobic respiration capacity during desiccation. Under air exposure stress, Chinese mitten crabs change their energy utilization mode to meet their energy demands and adjust their respiratory metabolism and antioxidant enzymes activities to adapt to adverse environments.

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Section: Methodssupporting

confidence: 63%

Respiratory Metabolism and Antioxidant Response in Chinese Mitten Crab Eriocheir sinensis During Air Exposure and Subsequent Reimmersion

Bao

Xing

et al. 2019

Front. Physiol.

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“…Due to the high lethality of BMD and the complexity of the weather from April to May, the different tissues of P. clarkii are adversely affected by pathogens and environmental stressors, as well as the resulting gene expression changes. Gills are the main respiratory-related organs of aquatic crustaceans and thus play an important role in the regulation of external osmotic pressure, ion transport, and immune response [18][19][20] . Based on our transcriptome data, the gills of diseased P. clarkii presented the highest number of differentially expressed genes compared to the two other tissues examined herein (hepatopancreas and muscle).…”

Section: Discussionmentioning

confidence: 99%

“…KEGG enrichment analysis was conducted on the DEGs identified in different tissues. Gills are the main respiratory-related organs of aquatic crustaceans, and therefore play an important role in external osmotic pressure regulation, ion transport, and immune response [18][19][20] . KEGG enrichment results ( Fig.…”

Section: Go and Kegg Enrichment Analysis Of Degsmentioning

confidence: 99%

Transcriptomic analysis of Procambarus clarkii affected by “Black May” disease

Shen

Zhang

Gong

et al. 2020

Sci Rep

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Each year from April to May, high mortality rates are reported in red swamp crayfish (Procambarus clarkii) cultured in Jiangsu and other regions, in China, and this phenomenon has come to be known as “Black May” disease (BMD). Therefore, in order to investigate the possible causes of this disease, this study gathered BMD-affected P. clarkii samples and performed transcriptome analysis on hepatopancreas, gill, and muscle tissues. A total of 19,995,164, 149,212,804, and 222,053,848 clean reads were respectively obtained from the gills, muscle, and hepatopancreas of BMD-affected P. clarkii, and 114,024 unigenes were identified. The number of differentially expressed genes (DEGs) in gill, muscle, and hepatopancreas was 1703, 964, and 476, respectively. GO and KEGG enrichment analyses of the DEGs were then conducted. Based on KEGG pathway enrichment analysis, the most significantly differentially expressed pathways were mainly those involved with metabolism, human disease, and cellular processes. Further analysis of the significantly DEGs revealed that they were mainly related to the mitochondrial-mediated apoptosis pathway and that the expression of these DEGs was mostly down-regulated. Moreover, the expression of genes related to immune and metabolism-related pathways was also significantly down-regulated, and these significantly-inhibited pathways were the likely causes of P. clarkii death. Therefore, our results provide a basis for the identification of BMD causes.

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“…Genes with unique sequences (unigenes) were annotated and functionally classified based on Clusters of Orthologous Groups of proteins (COG), KOG, and eggNOG protein sequence data libraries for manual annotation and reviewed against Swiss-Prot. Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases [45].…”

Section: Sequence Annotation and Functional Classificationmentioning

confidence: 99%

Transcriptome analysis of Curcuma wenyujin from Haikou and Wenzhou, and a comparison of the main constituents and related genes of Rhizoma Curcumae

Liu

Yang

et al. 2020

PLoS ONE

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For more than a thousand years, Rhizoma Curcumae (known as E zhu), a Chinese herbal medicine, has been used to eradicate blood stasis and relieve aches. The plant Curcuma wenyujin, which is grown primarily in Wenzhou, China, is considered the best source of Rhizoma Curcumae. In this study, we sought to ascertain differences in transcript profiles of C. wenyujin grown in traditional (Wenzhou) and recently established (Haikou) production areas based on Illumina and RNA (RNA-seq) sequencing. We also examined differences in the main components of the volatile oil terpene; curcumin, polysaccharide, and starch constituents and related genes in the corresponding pathways, in C. wenyujin cultivated in the two production areas. We accordingly found that the essential oil (2.05%), curcumin (1.46%), and polysaccharide (8.90%) content in Wenzhou rhizomes was higher than that in the rhizomes of plants from Haikou (1.60%, 0.91%, and 6.15%, respectively). In contrast, the starch content of Wenzhou rhizomes (17.0%) was lower than that of Haikou rhizomes (23.8%). Furthermore, we detected significant differences in the oil components of Haikou and Wenzhou rhizomes, with curzerene (32.34%), curdione (21.35%), and germacrene B (9.39%) being the primary components of the essential oil derived from Wenzhou rhizomes, and curzerene (20.13%), curdione (14.73%), and cineole (9.76%) being the main constituents in Haikou rhizomes. Transcriptome and qPCR analyses revealed considerable differences in gene expression between Wenzhou and Haikou rhizomes. The expression of terpene, curcumin, and polysaccharide pathway-related genes in Wenzhou rhizomes was significantly up-regulated, whereas the expression of starch-associated genes was significantly down-regulated, compared with those in Haikou rhizomes. Difference in the content of terpene, curcumin, polysaccharides, and starch in rhizomes from the two production areas could be explained in terms of differences in expression of the related genes.

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Identification of immune-related genes in gills of Chinese mitten crabs (Eriocheir sinensis) during adaptation to air exposure stress

Cited by 28 publications

References 57 publications

Respiratory Metabolism and Antioxidant Response in Chinese Mitten Crab Eriocheir sinensis During Air Exposure and Subsequent Reimmersion

Respiratory Metabolism and Antioxidant Response in Chinese Mitten Crab Eriocheir sinensis During Air Exposure and Subsequent Reimmersion

Transcriptomic analysis of Procambarus clarkii affected by “Black May” disease

Transcriptome analysis of Curcuma wenyujin from Haikou and Wenzhou, and a comparison of the main constituents and related genes of Rhizoma Curcumae

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