Identification of IgE binding components of two major weed pollens, ragweed and mugwort

Yoon, Moon-Gyung; Kim, Mi Ae; Jin, Hongzhe; Shin, Yoo-Seob; Park, Hae‐Sim

doi:10.4168/aard.2014.2.5.337

Cited by 4 publications

(3 citation statements)

References 22 publications

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Section: Introductionmentioning

confidence: 99%

“…Ambrosia artemisiifolia, also known as common or short ragweed is an invasive plant originating from North America and now occurring worldwide, including certain parts of Europe and Asia. [1][2][3] In Europe, ragweed became domestic in the Pannonian Plain and parts of the surrounding countries such as Austria, 4 Slovakia, 5 Serbia, 6,7 Croatia, 6,8 Slovenia, 6 Hungary 9 and Romania, 6,10,11 in the Rhône Valley and northern Italy. 12,13 In these areas high sensitization rates among atopic patients could be observed, 60% in Hungary, 14 47% in the Rhône Valley area, 15 and 70% in northern Italy.…”

Section: Introductionmentioning

confidence: 99%

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Complex IgE sensitization patterns in ragweed allergic patients: Implications for diagnosis and specific immunotherapy

Buzan

Zbîrcea

Gattinger

et al. 2022

Clinical & Translational All

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Background Ragweed (Ambrosia artemisiifolia) is one of the most important allergen sources, worldwide, causing severe respiratory allergic reactions in late summer and fall, in sensitized patients. Amb a 1 has been considered as the most important allergen in ragweed but 12 ragweed pollen allergens are known. The aim of our study was to investigate IgE reactivity profiles of ragweed allergic patients and to associate them with clinical symptoms. Methods IgE sensitization profiles from clinically well‐characterized ragweed allergic patients (n = 150) were analyzed using immunoblotted ragweed pollen extract. Immunoblot inhibition experiments were performed with two Amb a 1 isoforms and CCD markers and basophil activation experiments were performed with IgE serum before and after depletion of Amb a 1‐specific IgE. Results By IgE‐immunoblotting 19 different IgE reactivity patterns with and without Amb a 1‐sensitization were found. The majority of patients (>95%) suffered from rhino‐conjunctivitis, around 60% reported asthma‐like symptoms and about 25% had skin reactions. Patients with complex IgE sensitization profiles tended to have more clinical symptoms. Serum with and without Amb a 1‐specific IgE induced basophil activation. Conclusions Ragweed pollen allergic patients exhibit complex IgE reactivity profiles to ragweed allergens including Amb a 1 isoforms and cross‐reactive carbohydrates indicating the importance of Amb a 1 isoforms and additional allergens for diagnosis and allergen‐specific immunotherapy of ragweed allergy.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Complex IgE sensitization patterns in ragweed allergic patients: Implications for diagnosis and specific immunotherapy

Buzan

Zbîrcea

Gattinger

et al. 2022

Clinical & Translational All

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“…Short ragweed (Ambrosia artemisiifolia) is an invasive plant native to North America that has spread worldwide [1][2][3][4][5][6][7][8][9][10][11][12][13]. Due to its high allergenicity, ragweed pollen has become a significant health issue in infested areas, causing severe respiratory symptoms [14].…”

Section: Introductionmentioning

confidence: 99%

Insect Cell-Expressed Major Ragweed Allergen Amb a 1.01 Exhibits Similar Allergenic Properties to Its Natural Counterpart from Common Ragweed Pollen

Buzan,

Grijincu,

Zbîrcea

et al. 2024

IJMS

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Common ragweed pollen allergy has become a health burden worldwide. One of the major allergens in ragweed allergy is Amb a 1, which is responsible for over 90% of the IgE response in ragweed-allergic patients. The major allergen isoform Amb a 1.01 is the most allergenic isoform in ragweed pollen. So far, no recombinant Amb a 1.01 with similar allergenic properties to its natural counterpart (nAmb a 1.01) has been produced. Hence, this study aimed to produce a recombinant Amb a 1.01 with similar properties to the natural isoform for improved ragweed allergy management. Amb a 1.01 was expressed in insect cells using a codon-optimized DNA construct with a removable N-terminal His-Tag (rAmb a 1.01). The recombinant protein was purified by affinity chromatography and physicochemically characterized. The rAmb a 1.01 was compared to nAmb a 1.01 in terms of the IgE binding (enzyme-linked immunosorbent assay (ELISA), immunoblot) and allergenic activity (mediator release assay) in well-characterized ragweed-allergic patients. The rAmb a 1.01 exhibited similar IgE reactivity to nAmb a 1.01 in different IgE-binding assays (i.e., IgE immunoblot, ELISA, quantitative ImmunoCAP inhibition measurements). Furthermore, the rAmb a 1.01 showed comparable dose-dependent allergenic activity to nAmb a 1.01 regarding basophil activation. Overall, the results showed the successful expression of an rAmb a 1.01 with comparable characteristics to the corresponding natural isoform. Our findings provide the basis for an improvement in ragweed allergy research, diagnosis, and immunotherapy.

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Ragweed is in the Air: Ambrosia L. (Asteraceae) and Pollen Allergens in a Changing World

Montagnani

Gentili

Citterio

2023

CPPS

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Globally Ambrosia species (Asteraceae), commonly called ragweed, are recognized to be one of the most problematic groups of invasive weeds and one of the main allergenic genus. Climate and land-use change and air pollution are expected to promote ragweed spread, increase airborne ragweed pollen concentrations (the source of allergens), extend the pollen season, and promote long-distance transport of pollen or sub-pollen particles containing allergens. The allergenicity of pollen itself is going to increase. Likely, all these factors will have meaningful effects in the exacerbation of the sensitization to ragweed pollen and the severity of allergy symptoms. Globally the major health concern regards A. artemisiifolia, because of its very wide global distribution and highly invasive behavior. Together with A. artemisiifolia, also A. trifida and A. psilostachya are species of health concern distributed across different continents, widespread and invasive in several regions. The present review summarizes the characteristics of these species and gives an overview of factors contributing to their allergenicity.

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Identification of IgE binding components of two major weed pollens, ragweed and mugwort

Cited by 4 publications

References 22 publications

Complex IgE sensitization patterns in ragweed allergic patients: Implications for diagnosis and specific immunotherapy

Complex IgE sensitization patterns in ragweed allergic patients: Implications for diagnosis and specific immunotherapy

Insect Cell-Expressed Major Ragweed Allergen Amb a 1.01 Exhibits Similar Allergenic Properties to Its Natural Counterpart from Common Ragweed Pollen

Ragweed is in the Air: Ambrosia L. (Asteraceae) and Pollen Allergens in a Changing World

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