Identification of IgE and IgG binding epitopes on β‐ and κ‐casein in cow's milk allergic patients

Chatchatee, Pantipa; Järvinen, Kirsi-Marjut; Bardina, L.; Vila, Leticia; Beyer, Kirsten; Sampson, Hugh A.

doi:10.1046/j.1365-2222.2001.01167.x

Cited by 198 publications

(145 citation statements)

References 14 publications

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“…This has also been observed in other CMA studies [24, 25]. For milk allergy, IgE binding to certain linear epitopes seem to predict persistence of allergy [26,27,28]. However, it cannot predict the time point when tolerance has developed [13].…”

Section: Discussionsupporting

confidence: 56%

Reintroduction of Cow’s Milk in Milk-Allergic Children: Safety and Risk Factors

Vassilopoulou

Konstantinou²,

Kassimos³

et al. 2008

Int Arch Allergy Immunol

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Background: Although development of clinical tolerance is the rule in allergy to cow’s milk (CM), food challenges are required in order to reintroduce CM into the patient’s diet. Less ‘invasive’ procedures able to predict tolerance would be useful as clinical tools. The purpose of this study was to identify potential risk factors for clinical reactivity in CM-allergic children assessed for CM reintroduction. Methods: One hundred and sixteen open challenges performed in children 10–47 months old with IgE-mediated allergy to CM, in order to reintroduce CM into the diet, were retrospectively evaluated. Specific IgE (sIgE) levels assessed by the CAP System FEIA and skin prick tests (SPT) were obtained at diagnosis and prechallenge. Demographic parameters and measures of sIgE were evaluated as potential predictors of a positive challenge in univariate and multivariate logistic regression models. Results: Twenty-four out of 116 challenges were positive, 9 of which required the use of adrenaline. In order of performance, prechallenge sIgE <3.94 kU/l, the combination of SPT and sIgE or an SPT wheal <4 mm could correctly predict a negative challenge outcome, whereas values of SPT >7.5 mm or sIgE >25.4 kU/l, or their combination, had a high positive predictive value. The presence of atopic dermatitis did not affect the predictive accuracy of these values. Conclusions: Milk sIgE level prechallenge is a useful predictor of challenge outcome in patients with milk allergy. SPT do not offer an additional predictive value, but can be used when sIgE is not available.

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Section: Discussionsupporting

confidence: 56%

Reintroduction of Cow’s Milk in Milk-Allergic Children: Safety and Risk Factors

Vassilopoulou

Konstantinou²,

Kassimos³

et al. 2008

Int Arch Allergy Immunol

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“…In conclusion, this report completes the mapping of the sequential B-cell epitopes of the major CM proteins [5, 6, 7]. The next steps will include the determination of the AA crucial for IgE binding.…”

Section: Discussionmentioning

confidence: 67%

“…Previous work done by our group has identified the IgE-binding regions on α s1 -, β-, and ĸ-casein [5, 6]as well as on two major whey proteins [7]. In this study, overlapping synthetic peptides were used to identify IgE-binding regions of α s2 -casein in CMA patients.…”

Section: Introductionmentioning

confidence: 99%

Identification of Sequential IgE-Binding Epitopes on Bovine α<sub>s2</sub>-Casein in Cow’s Milk Allergic Patients

Busse

Järvinen

Vila

et al. 2002

Int Arch Allergy Immunol

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Background: Caseins are the major allergens responsible for cow’s milk allergy (CMA). We have previously identified the IgE-binding epitopes of the major cow’s milk (CM) proteins except for α_s2-casein. Methods: Overlapping decapeptides representing the entire length of α_s2-casein were synthesized on a cellulose-derivatized membrane. Sera from 13 CM-allergic children, 4–15 years of age, with a median level of CM-specific IgE >100 kU/l (range 33.7 to > 100 kU/l) were used to identify IgE-binding epitopes. Results: Four major and six minor sequential IgE-binding regions were identified on α_s2-casein. The first major region is located in the middle of the protein at amino acids (AA) 83–100, and the other three major regions are located in the carboxy terminal portion of the protein at AA 143–158, 157–172 and 165–188. The minor IgE-binding regions were identified at AA 31–44, 43–56, 93–106, 105–114, 117–128, and 191–200. Conclusion: We identified 10 sequential IgE-binding regions on α_s2-casein and performed the first crucial step in the development of immunotherapeutic interventions for CMA.

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“…Caseins retain their IgE reactivity irrespective of their phosphorylation state. The IgE binding regions have been identified in caseins with six major and three minor epitopes found for s1 -casein (Chatchatee et al, 2001a), four major and six minor epitopes found for s2 -casein (Busse et al, 2001), six major and three minor epitopes for -casein and eight major epitopes for -casein (Chatchatee et al, 2001b). These epitopes are thermostable, possibly a reflection of the rheomorphic character of this protein.…”

Section: Protein Allergens Of Animal Originmentioning

confidence: 94%