Identification of<i>Yersinia enterocolitica</i>at the Species and Subspecies Levels by Fourier Transform Infrared Spectroscopy

Kuhm, Andrea Elisabeth; Suter, D.; Felleisen, Richard; Rau, Jörg

doi:10.1128/aem.00206-09

Cited by 69 publications

(68 citation statements)

References 29 publications

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“…For this purpose, bacterial isolates were cultivated on sheep blood agar plates (Oxoid) at 37°C for 24 h. Sample preparation, FT-IR spectroscopy (using IFS 28/B spectrometer, BrukerOptics, Ettlingen, Germany) and data acquisition were performed as described previously (Kuhm et al, 2009). Analysis of data was carried out using OPUS Software (vers.…”

Section: Fourier Transform Infrared Spectroscopy (Ft-ir)mentioning

confidence: 99%

Outbreak of Staphylococcal Food Poisoning Due to SEA-ProducingStaphylococcus aureus

Johler

Tichaczek-Dischinger

Rau

et al. 2013

Foodborne Pathogens and Disease

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In 2008, 150 people gathered for a wedding celebration in Baden-Württemberg, Germany. Three hours after ingestion of a variety of foods including pancakes filled with minced chicken, several guests exhibited symptoms of acute gastroenteritis such as vomiting, diarrhea, fever, and ague. Twelve guests were reported to have fallen ill, with nine of these seeking medical care in hospitals. At least four patients were admitted to the hospital and received inpatient treatment, among them a 2-year-old child and a woman in the 4th month of pregnancy. Within 24 h of the event, an investigative team collected a variety of samples including refrigerated leftovers, food in the storage unit of the caterer, nasal swabs of the caterer, as well as 21 environmental swabs. Five stool samples from patients were provided by the hospitals. Staphylococcus aureus isolates were gathered from eight samples, among them nasal swabs of the caterer, food samples, and one stool sample. Fourier transform-infrared spectroscopy was used for species identification and for primary clustering of the isolates in a similarity tree. The isolates were further characterized by spa typing and pulsed-field gel electrophoresis, and a DNA microarray was used to determine the presence/absence of genes involved in virulence and antimicrobial resistance. We were able to match an enterotoxigenic strain from the stool sample of a patient to isolates of the same strain obtained from food and the nasal cavity of a food handler. The strain produced the enterotoxin SEA and the toxic shock syndrome toxin-1, and was also found to exhibit the genes encoding enterotoxins SEG and SEI, as well as the enterotoxin gene cluster egc. This is one of only a few studies that were able to link a staphylococcal food poisoning outbreak to its source. In 2008, 150 people gathered for a wedding celebration in Baden-Wü rttemberg, Germany. Three hours after ingestion of a variety of foods including pancakes filled with minced chicken, several guests exhibited symptoms of acute gastroenteritis such as vomiting, diarrhea, fever, and ague. Twelve guests were reported to have fallen ill, with nine of these seeking medical care in hospitals. At least four patients were admitted to the hospital and received inpatient treatment, among them a 2-year-old child and a woman in the 4th month of pregnancy.Within 24 h of the event, an investigative team collected a variety of samples including refrigerated leftovers, food in the storage unit of the caterer, nasal swabs of the caterer, as well as 21 environmental swabs. Five stool samples from patients were provided by the hospitals. Staphylococcus aureus isolates were gathered from eight samples, among them nasal swabs of the caterer, food samples, and one stool sample. Fourier transforminfrared spectroscopy was used for species identification and for primary clustering of the isolates in a similarity tree. The isolates were further characterized by spa typing and pulsed-field gel electrophoresis, and a DNA microarray was used to determin...

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Section: Fourier Transform Infrared Spectroscopy (Ft-ir)mentioning

confidence: 99%

Outbreak of Staphylococcal Food Poisoning Due to SEA-ProducingStaphylococcus aureus

Johler

Tichaczek-Dischinger

Rau

et al. 2013

Foodborne Pathogens and Disease

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“…Species FTIRS HCA, ANN, PCA, SIMCA [45,49,53,86,92] Stenotrophomonas maltophilia Species FTIRS PCA, ANN [45,55] Ralstonia pickettii Species FTIRS ANN [45] Achromobacter spp Species FTIRS ANN [45] Yersinia spp. Species FTIRS, FTIRS-ATR ANN, SIMCA, PCA, CVA [47,50,52] Yersinia enterocolitica Serotype FTIRS ANN [47] Biotype FTIRS ANN [47] Yersinia ruckeri Biotype FTIRS ANN [80] Oscillatoria limosa Species FTIRS-ATR PCA, ANN [48] Arthrospira platensis Species FTIRS-ATR PCA, ANN [48] Phormidium spp. Species FTIRS-ATR PCA, ANN [48] Scytonem javanicum Species FTIRS-ATR PCA, ANN [48] Nostoc punctiform Species FTIRS-ATR PCA, ANN [48] Salmonella spp.…”

Section: Bacteria Discrimination Level Infrared Technique Chemometricmentioning

confidence: 99%

“…However, one year later, Coutinho et al [46] showed that FTIRS could lead to a high rate of misidentifications of B. cepacia complex species. In 2009, Kuhm and collaborators [47] successfully discriminated 4 Yersinia species based on their IR spectra and ANN. In 2004, seven cyanobacterial strains belonging to five different genus and/or species were also successfully discriminate by FTIRS-ATR and ANN (Bounphanmy and co-workers [48] ).…”

Section: Bacteria Discrimination Level Infrared Technique Chemometricmentioning

confidence: 99%

“…However, they considered in the analysis only one isolate per serotype which might be unsufficient considering the chemometric-based approach. [61][62][63] FTIRS combined with appropriate chemometric methods were also able to discriminate several serotypes of Streptococcus pneumonia, [64] S. aureus, [65] and Y. enterocolitica [47] and three Legionella pneumophila serogroups. [66] E. coli [67,68] and A. baumannii [69,70] were successfully discriminated according to their sequence types by IR spectroscopy and chemometrics, E. faecium isolates according their PFGE type [71] and E. coli according to their MLVA profiles.…”

Section: Bacterial Typing At the Subspecies Levelmentioning

confidence: 99%

“…IR spctroscopy proved also to be able to discriminate Brucella spp. isolates according to their biovars, [78] Xanthomonas oryzae pathovars, [79] B. cepacia complex species ribopatterns, [46] L. monocytogenes halotypes, [57] Y. enterocolitica and Yersinia ruckeri biotypes, [47,80] S. enterica serovar Enteritidis phage types, [81] and Campilobacter spp. genotypes.…”

Section: Bacterial Typing At the Subspecies Levelmentioning

confidence: 99%

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An Overview of the Evolution of Infrared Spectroscopy Applied to Bacterial Typing

Quintelas

Ferreira

Lopes

et al. 2017

Biotechnology Journal

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The sustained emergence of new declared bacterial species makes typing a continuous challenge for microbiologists. Molecular biology techniques have a very significant role in the context of bacterial typing, but they are often very laborious, time consuming, and eventually fail when dealing with very closely related species. Spectroscopic-based techniques appear in some situations as a viable alternative to molecular methods with advantages in terms of analysis time and cost. Infrared and mass spectrometry are among the most exploited techniques in this context: particularly, infrared spectroscopy emerged as a very promising method with multiple reported successful applications. This article presents a systematic review on infrared spectroscopy applications for bacterial typing, highlighting fundamental aspects of infrared spectroscopy, a detailed literature review (covering different taxonomic levels and bacterial species), advantages, and limitations of the technique over molecular biology methods and a comparison with other competing spectroscopic techniques such as MALDI-TOF MS, Raman, and intrinsic fluorescence. Infrared spectroscopy possesses a high potential for bacterial typing at distinct taxonomic levels and worthy of further developments and systematization. The development of databases appears fundamental toward the establishment of infrared spectroscopy as a viable method for bacterial typing.

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The Impact of Bacterial Surface Polymers on Bacterial Adhesion

Liu

2013

Polymer Adhesion, Friction, and Lubrication

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Identification ofYersinia enterocoliticaat the Species and Subspecies Levels by Fourier Transform Infrared Spectroscopy

Cited by 69 publications

References 29 publications

Outbreak of Staphylococcal Food Poisoning Due to SEA-ProducingStaphylococcus aureus

Outbreak of Staphylococcal Food Poisoning Due to SEA-ProducingStaphylococcus aureus

An Overview of the Evolution of Infrared Spectroscopy Applied to Bacterial Typing

The Impact of Bacterial Surface Polymers on Bacterial Adhesion

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