Identification of <i>Theileria</i> spp. and investigation of hematological profiles of their infections in goats in Hainan Island, China

Yang, Lu; Wang, Jinhua; Upadhyay, Archana; Zhao, Jianguo; Huang, Liang-Yuan; Liao, Chenghong; Han, Qian

doi:10.1051/parasite/2022013

Cited by 8 publications

(7 citation statements)

References 22 publications

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“…Piroplasma and Anaplasma detection rates significantly differed among the location of the goats. The same was observed in goat herds from China [ 29 ], Tunisia [ 40 ], and Oman [ 44 ]. Location-specific factors in this study, such as management practices and macroclimatic conditions that affect tick proliferation, may be attributed to the different sampling sites characterized by diverse topography, terrain, microclimate, and fauna.…”

Section: Discussionsupporting

confidence: 66%

“…Herein, we present the first molecular identification of Babesia, Theileria, and Anaplasma in goats from the Philippines. A high detection rate of piroplasma DNA was recorded in goats (77.02%), which was higher than caprine Babesia and Theileria rates recorded in Pakistan (5–40.80%) [ 25 , 26 ], Turkey (21.40%) [ 27 ], Italy (11.70%) [ 3 ], China (11.90–34.70%) [ 28 , 29 ], Ethiopia (1.90%) [ 30 ], and Tunisia (4.70%) [ 31 ], while comparable to that from Malawi (72.70%) [ 32 ]. The detection rate (38.64%) of Anaplasma spp.…”

Section: Discussionmentioning

confidence: 99%

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First Molecular Identification of Babesia, Theileria, and Anaplasma in Goats from the Philippines

et al. 2022

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Goats are key livestock animals and goat raising is an income-generating venture for smallholder farmers, supporting agricultural development in many parts of the world. However, goat production is often limited by various factors, such as tick-borne diseases. Goat piroplasmosis is a disease caused by apicomplexan parasites Babesia spp. and Theileria spp., while anaplasmosis is caused by bacterial Anaplasma spp. In the Philippines, the presence of Babesia, Theileria, and Anaplasma has not been reported in goats. In this study, DNA obtained from goats were molecularly screened for Babesia/Theileria and Anaplasma. Of 396, 77.02% (305/396) and 38.64% (153/396) were positive for piroplasma and Anaplasma using PCR assays targeting the 18S rRNA and 16S rRNA genes, respectively. Similarly, Babesia ovis was detected in six samples (1.52%). Representative Babesia/Theileria sequences shared 89.97–97.74% identity with each other and were most closely related to T. orientalis, T. annulata, and Theileria spp. Meanwhile, Anaplasma 16SrRNA sequences were related to A. odocoilei, A. platys, and A. phagocytophilum. This is the first molecular identification of B. ovis, Theileria spp., and Anaplasma spp. in goats from the Philippines.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

First Molecular Identification of Babesia, Theileria, and Anaplasma in Goats from the Philippines

et al. 2022

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“…A previous study reported a prevalence of 5% of T. luwenshuni in the Rayong and Chonburi provinces of Thailand [43]. T. luwenshuni is widely distributed in the small ruminant population in China [19,21,31,41], but its distribution in Thai goats is unknown. The results of the phylogenetic analysis revealed that the T. luwenshuni isolates in this research were closely related to T. luwenshuni isolates from China, suggesting that the parasite may have invaded Thailand through the passage of infected tick vectors or animals from China.…”

Section: Discussionmentioning

confidence: 98%

“…In the neighbouring countries, the prevalence rates of Theileria spp. In goats was 34.7-52.7% from China [19,40,41], 33.3% from Myanmar [24], 8.5% from Bangladesh [42] and 13.4% from Uganda [37].…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection and Genetic Diversity of Tick-Borne Pathogens in Goats from the Southern Part of Thailand

2022

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Tick-borne hemoprotozoan and rickettsial diseases affect the health and productivity of small ruminants in tropical and subtropical regions. Despite the large population of goats in the southern part of Thailand, there is limited information on the prevalence of tick-borne pathogens. In this study, polymerase chain reaction was used to detect the presence of Theileria spp., T. ovis, T. orientalis, Babesia ovis, Anaplasma ovis, and A. marginale in 262 goats from three provinces in the southern part of Thailand. In this investigation, Theileria spp. and A. ovis were detected while T. ovis, B. ovis, and A. marginale were not detected. Overall infection rates of Theileria spp. and A. ovis were 10.3% and 1.5%, respectively. The co-infections of two parasites was observed in 1.5% of goats. Sequence analysis showed the presence of T. luwenshuni and T. orientalis in the goat samples. This study is the first to use the molecular detection of T. orientalis in Thai goats, and presents genetic characterization using the major piroplasm surface protein (MPSP) gene. In the phylogenetic analysis, the T. orientalis MPSP sequence was classified as type 7. The A. ovis major surface protein 4 (MSP4) gene sequences shared high identities and similarity with each other and clustered with isolates from other regions. This study provides information about the prevalence and genetic diversity of tick-borne pathogens in goats in the study area, and is expected to be valuable for the development of effective control measures to prevent disease in animals in Thailand.

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“…Recent studies have highlighted the continued prevalence and impact of this disease on domestic ruminant production, especially compared to cattle. In China, for example, a study by Yang et al . (2022) found that Theileria infections were common among goats, while a recent study by Rahravani et al .…”

Section: Introductionmentioning

confidence: 99%

Differential diagnosis of theileriosis through blood smear examination and polymerase chain reaction in small ruminants from Pakistan

Riaz¹,

Nasreen²,

Khan³

et al. 2023

Open Vet J

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Background: Ovine and caprine theileriosis is a tick-borne hemoprotozoan disease, caused by Theileria spp., responsible for heavy economic losses in terms of high mortality and morbidity rates. Diagnosis of ovine theileriosis is primarily based on clinical symptoms, microscopic screening of stained blood smears and lymph node biopsy smears, but the limitations of these detection methods against Theileria spp. infection limit their specificity. Aim: To overcome these limitations, the current study reports the differential diagnosis of theileriosis through Blood Smear Examination and Polymerase Chain Reaction in small ruminants from Pakistan. Methods: The study was conducted on 1200 apparently healthy small ruminants (737 sheep and 463 goats). Firstly, blood smears were screened for the presence of Theileria piroplasms in red blood cells. Secondly, PCR amplification based on 18S rRNA gene was performed by using primers specific to Theileria spp. Results: Out of 1200 small ruminants, 100 were positive for Theileria genus by blood smears microscopy while 315 samples were found positive by PCR. The sensitivity and specificity rates were 26.98 % and 98.31 % for blood smear method, and 73.01% and 100 % of PCR assay, respectively. Conclusion: Our finding suggests that PCR is the gold standard method compared to the conventional method of smear examination for the diagnosis of ovine and caprine theileriosis in Pakistan.

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Identification of Theileria spp. and investigation of hematological profiles of their infections in goats in Hainan Island, China

Cited by 8 publications

References 22 publications

First Molecular Identification of Babesia, Theileria, and Anaplasma in Goats from the Philippines

First Molecular Identification of Babesia, Theileria, and Anaplasma in Goats from the Philippines

Molecular Detection and Genetic Diversity of Tick-Borne Pathogens in Goats from the Southern Part of Thailand

Differential diagnosis of theileriosis through blood smear examination and polymerase chain reaction in small ruminants from Pakistan

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