Identification of Staphylococcus aureus virulence genes in a murine model of bacteraemia using signature‐tagged mutagenesis

Host defenses againstStaphylococcus aureusinfection require recognition of bacterial lipoproteins

“…Additionally, a signature-tagged mutagenesis screen of S. aureus identified lsp as a factor contributing to virulence; however, the calculated LD 50 of this lsp mutant in an animal model of i.p. infection was found to be similar to that of the wild-type parental strain (30). Thus, the increased virulence of lgt mutants appears to be solely caused by the lack of Lgt activity that results in a loss of lipoprotein acylation, not signal sequence removal.…”

Maturation of Lipoproteins by Type II Signal Peptidase Is Required for Phagosomal Escape of Listeria monocytogenes

“…In vivo screenings for attenuated mutants by signature-tagged mutagenesis, in Staphylococcus aureus, identified transposon insertions into the lsp gene (32,33). However, the effect of lsp inactivation on virulence was very modest.…”

Characterisation of an acapsular mutant of Burkholderia pseudomallei identified by signature tagged mutagenesis

“…Signature tagged mutagenesis (STM) has been used successfully in several bacterial pathogens to identify virulence determinants in vivo [25][26][27][28][29][30][31][32][33]. Briefly, a pool of transposons is assembled, each harbouring a unique oligonucleotide tag.…”