Identification of
            <i>Salmonella</i>
            with the O-1 Bacteriophage

Welkos, Susan L.; Schreiber, Minnie; Baer, Herman

doi:10.1128/am.28.4.618-622.1974

Cited by 32 publications

(3 citation statements)

References 10 publications

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“…In 2011, it was classified as the type species of the Felixounavirus genus proposed by the ICTV [14]. Importantly, phages of Felixounavirus genus have been shown to infect several different types of Salmonella serovars [15,16]. Further reports of phages of the Felixounavirus genus have shown their potential for infecting Salmonella of numerous serovars, including descriptions of isolations from several countries such as Russia, the United States, and Chile, which were recovered in samples from different sources: chicken and bovine feces, sewage, and farm soil [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Felixounavirus Genomes Including Two New Members of the Genus That Infect Salmonella Infantis

et al. 2021

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Salmonella spp. is one of the most common foodborne pathogens worldwide; therefore, its control is highly relevant for the food industry. Phages of the Felixounavirus genus have the characteristic that one phage can infect a large number of different Salmonella serovars and, thus, are proposed as an alternative to antimicrobials in food production. Here, we describe two new members of the Felixounavirus genus named vB_Si_35FD and vB_Si_DR94, which can infect Salmonella Infantis. These new members were isolated and sequenced, and a subsequent comparative genomic analysis was conducted including 23 publicly available genomes of Felixounaviruses that infect Salmonella. The genomes of vB_Si_35FD and vB_Si_DR94 are 85,818 and 85,730 bp large and contain 129 and 125 coding sequences, respectively. The genomes did not show genes associated with virulence or antimicrobial resistance, which could be useful for candidates to use as biocontrol agents. Comparative genomics revealed that closely related Felixounavirus are found in distinct geographical locations and that this genus has a conserved genomic structure despite its worldwide distribution. Our study revealed a highly conserved structure of the phage genomes, and the two newly described phages could represent promising biocontrol candidates against Salmonella spp. from a genomic viewpoint.

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Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Felixounavirus Genomes Including Two New Members of the Genus That Infect Salmonella Infantis

et al. 2021

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“…Kentucky. Bacteriophage Felix 01 is a good example of a wide host range; it lyses nearly 98.5% of the tested Salmonella ( Welkos et al, 1974 ). However, using a phage cocktail might solve the limitation of a narrow host range bacteriophages.…”

Section: Resultsmentioning

confidence: 99%

“…The results showed that phage LPSent1 had a robust lytic activity with a wide host range against several strains of S. Enteritidis, S. Typhimurium and S. Kentucky. Bacteriophage Felix 01 is a good example of a wide host range; it lyses nearly 98.5% of the tested Salmonella (Welkos et al, 1974). However, using a phage cocktail might solve the limitation of a narrow host range bacteriophages.…”

Section: Host Rangementioning

confidence: 99%

Application of a novel lytic Jerseyvirus phage LPSent1 for the biological control of the multidrug-resistant Salmonella Enteritidis in foods

et al. 2023

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Non-typhoidal Salmonella is the tremendously predominant source of acquired foodborne infection in humans, causing salmonellosis which is a global threat to the healthcare system. This threat is even worse when it is combined with the incidence of multidrug-resistant Salmonella strains. Bacteriophage therapy has been proposed as a promising potential candidate to control a diversity of foodborne infective bacteria. The objective of this study designed to isolate and characterize lytic phages infecting zoonotic multi-drug resistant and strong biofilm producer Salmonella enterica serovar Enteritidis EG.SmE1 and then apply the isolated phage/s as a biocontrol agent against infections in ready-to-eat food articles including milk, water, apple juice, and chicken breasts. One lytic phage (LPSent1) was selected based on its robust and stable lytic activity. Phage LPSent1 belonged to the genus Jerseyvirus within the Jerseyvirinae subfamily. The lysis time of phage LPSent1 was 60 min with a latent period of 30 min and each infected cell burst about 112 plaque-forming units. Phage LPSent1 showed a narrow host range. Furthermore, the LPSent1 genome did not encode any virulence or lysogenic genes. In addition, phage LPSent1 had wide pH tolerance, prolonged thermal stability, and was stable in food articles lacking its susceptible host for 48 h. In vitro applications of phage LPSent1 inhibited free planktonic cells and biofilms of Salmonella Enteritidis EG.SmE1 with a lower occurrence to form phage-resistant bacterial mutants which suggests promising applications on food articles. Application of phage LPSent1 at multiplicities of infections of 100 or 1000 showed significant inhibition in the bacterial count of Salmonella Enteritidis EG.SmE1 by 5 log10/sample in milk, water, apple juice, and chicken breasts at either 4°C or 25°C. Accordingly, taken together these findings establish phage LPSent1 as an effective, promising candidate for the biocontrol of MDR Salmonella Enteritidis in ready-to-eat food.

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Isolation and partial characterization of ΦSP‐1, a Salmonella specific lytic phage from intestinal content of broiler chicken

Augustine

Louis²,

Varghese³

et al. 2012

J. Basic Microbiol.

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Salmonella enterica subsp. enterica serovar Enteritidis is a major causative agent of gastroenteritis with contaminated eggs and chicken meat being the major source of infection. Phages are seriously being considered as a safe and cheaper alternative to antibiotics. The intestinal content of chicken was used as source for isolating phages. Phage designated as ΦSP‐1 was selected for the study. Transmission electron microscopy (TEM) of phage ΦSP‐1 revealed that it belonged to family Podoviridae. The optimal multiplicity of infection (MOI) was 5 phages/cell. Latent and rise period were calculated to be 30 and 55 minutes respectively, while burst size was 44 phages/bacterial cell. The genome size of ΦSP‐1 was estimated to be 86 kb from pulsed‐field gel electrophoresis analysis (PFGE). The effect of different physical and chemical parameters like temperature, pH, salinity and CaCl2 were analyzed to optimize the conditions for large scale production of phages and to check the viability of ΦSP‐1 under different physiochemical conditions. A temperature of 40 °C, pH 8 and 0.25 M NaCl were found to be optimum for phage adsorption and it was able to survive up to a temperature of 50 °C for 3 min. Capability to survive under hostile environmental conditions, absence of virulence genes in genome and genus specificity suggest suitability of ΦSP‐1 to be used as a biocontrol agent.

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Identification of Salmonella with the O-1 Bacteriophage

Cited by 32 publications

References 10 publications

Comparative Analysis of Felixounavirus Genomes Including Two New Members of the Genus That Infect Salmonella Infantis

Comparative Analysis of Felixounavirus Genomes Including Two New Members of the Genus That Infect Salmonella Infantis

Application of a novel lytic Jerseyvirus phage LPSent1 for the biological control of the multidrug-resistant Salmonella Enteritidis in foods

Isolation and partial characterization of ΦSP‐1, a Salmonella specific lytic phage from intestinal content of broiler chicken

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