2005
DOI: 10.1128/jb.187.8.2912-2916.2005
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Identification of rcnA ( yohM ), a Nickel and Cobalt Resistance Gene in Escherichia coli

Abstract: We report here on the isolation and primary characterization of the yohM gene of Escherichia coli. We show that yohM encodes a membrane-bound polypeptide conferring increased nickel and cobalt resistance in E. coli. yohM was specifically induced by nickel or cobalt but not by cadmium, zinc, or copper. Mutation of yohM increased the accumulation of nickel inside the cell, whereas cells harboring yohM in multicopy displayed reduced intracellular nickel content. Our data support the hypothesis that YohM is the fi… Show more

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Cited by 176 publications
(183 citation statements)
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“…In addition, Co(II) was of particular interest to us because two other proteins involved in nickel homeostasis, RcnR and RcnA, also contribute to cobalt homeostasis in E. coli (33)(34)(35). To determine whether SlyD could coordinate Co(II), a direct cobalt titration of apo SlyD was analyzed via electronic absorption spectroscopy ( Figure 5).…”
Section: Co(ii) Binding To Slydmentioning
confidence: 99%
“…In addition, Co(II) was of particular interest to us because two other proteins involved in nickel homeostasis, RcnR and RcnA, also contribute to cobalt homeostasis in E. coli (33)(34)(35). To determine whether SlyD could coordinate Co(II), a direct cobalt titration of apo SlyD was analyzed via electronic absorption spectroscopy ( Figure 5).…”
Section: Co(ii) Binding To Slydmentioning
confidence: 99%
“…Similar processes are believed to be responsible for the increase in Co and Ni solubility and bio-uptake under sulfidic conditions of the natural environments [51,52]. Some microorganisms have developed metal efflux mechanisms, which pumps excess intracellular metals out of the cell as a strategy against metal toxicity when exposed to high concentrations of metals [53]. Although this topic is well studied in disciplines such as organic chemistry, biogeochemistry and medicine, only a few studies have targeted the effect of microbial stress response under metal deficiency/excess on metal speciation in biogas processes.…”
Section: Trace Metal Microbiology and Microbial Ecologymentioning
confidence: 99%
“…This observation suggests that Ni import by HupE is coupled to a proton motive force-dependent efflux system able to export Ni ions, whose activity is abolished in the presence of uncouplers, leading to an increase in the level of intracellular 63 Ni radioactivity. A possible candidate might be the RcnAB pump of E. coli 40,41 that has been demonstrated to export Ni and Co ions, helping the bacteria to overcome the toxicity of both metals when they are in excess. We do not know whether the basal level of RcnAB present in the conditions of our uptake assay would be sufficient to get rid of Ni, but measurement of HupE-mediated Ni transport in an rcnA-nikA background could help to clarify this.…”
Section: Energetics Of Transportmentioning
confidence: 99%