Identification of <i>Pasteurella canis</i> in a Soft Tissue Infection Caused by a Dog Bite: The First Report in Korea

Kim, Bongyoung; Pai, Hyunjoo; Lee, Kwang Hyun; Lee, Yangsoon

doi:10.3343/alm.2016.36.6.617

Cited by 11 publications

(11 citation statements)

References 10 publications

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“…aureus and P . aeruginosa , we observed increased activity for B1 (4–16 µg/mL) against other bacteria such as: Corynebacterium auriscanis associated with canine otitis externa 50 , Streptococcus canis which causes respiratory, cutaneous, genital and urinary infections in various animal species 51 , Pasteurella canis which is a well-known major pathogen of infections caused by dog bites 52 , and Enterococcus faecium which is an important nosocomial pathogen 53 . However, we observed no significant activity against bacterial isolates belonging to the species Acinetobacter baumannii , Escherichia coli , Enterococcus faecilis and Klebsiella pneumoniae .…”

Section: Discussionmentioning

confidence: 88%

Characterization, mechanism of action and optimization of activity of a novel peptide-peptoid hybrid against bacterial pathogens involved in canine skin infections

Greco

Emborg

Jana

et al. 2019

Sci Rep

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Integumentary infections like pyoderma represent the main reason for antimicrobial prescription in dogs. Staphylococcus pseudintermedius and Pseudomonas aeruginosa are frequently identified in these infections, and both bacteria are challenging to combat due to resistance. To avoid use of important human antibiotics for treatment of animal infections there is a pressing need for novel narrow-spectrum antimicrobial agents in veterinary medicine. Herein, we characterize the in vitro activity of the novel peptide-peptoid hybrid B1 against canine isolates of S . pseudintermedius and P . aeruginosa . B1 showed potent minimum inhibitory concentrations (MICs) against canine S . pseudintermedius and P . aeruginosa isolates as well rapid killing kinetics. B1 was found to disrupt the membrane integrity and affect cell-wall synthesis in methicillin-resistant S . pseudintermedius (MRSP). We generated 28 analogues of B1 , showing comparable haemolysis and MICs against MRSP and P . aeruginosa . The most active analogues ( 23 , 26 ) and B1 were tested against a collection of clinical isolates from canine, of which only B1 showed potent activity. Our best compound 26 , displayed activity against P . aeruginosa and S . pseudintermedius , but not the closely related S . aureus . This work shows that design of target-specific veterinary antimicrobial agents is possible, even species within a genus, and deserves further exploration.

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Section: Discussionmentioning

confidence: 88%

Characterization, mechanism of action and optimization of activity of a novel peptide-peptoid hybrid against bacterial pathogens involved in canine skin infections

Greco

Emborg

Jana

et al. 2019

Sci Rep

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“…Pasteurella canis is a gram negative, facultative anaerobic coccobacillus found in the oral and gastrointestinal flora of dogs and cats [4] . This non-motile bacterium is commonly associated with skin and soft tissue infections associated with dog bites and scratches [5] .…”

Section: Discussionmentioning

confidence: 99%

“…Antimicrobial therapy is modified based on the results of gram stains, bacterial (aerobic and anaerobic) and/or fungal cultures and sensitivities. Pasteurella species are generally susceptible to most antibiotics including penicillin G, amoxicillin/clavulanic acid, piperacillin/tazobactam, doxycycline, fluoroquinolones, third and fourth generation cephalosporins, and carbabenems [4] . On the other hand, macrolides, first generation cephalosporins, and aminoglycosides have poor activity [10] .…”

Section: Discussionmentioning

confidence: 99%

Medical and surgical management of Pasteurella canis infectious keratitis

Shah

Talati

Mauger

2017

IDCases

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IntroductionWe describe a case of refractory Pasteurella Canis keratitis that initially failed antimicrobial treatment but was successfully treated with a conjunctival flap followed by a penetrating keratoplasty.Case discussionA 70-year-old male had persistent corneal edema related to previous herpes zoster keratouveitis with visual acuity of 20/400. An endothelial keratoplasty was performed with improvement in vision to 20/25. Several months after this surgery the patient developed a corneal infiltrate and cultures grew Pasturella canis. The infection persisted despite topical treatment with broad spectrum fortified vancomycin, tobramycin and fluoroquinolones. A conjunctival flap combined with corneal cryotherapy was performed to help mitigate symptoms and to allow the ulcer to heal. A penetrating keratoplasty was done several months later.DiscussionThe patient achieved pain relief immediately after the flap was placed. Medications were tapered off in the following few months after the procedure. A penetrating keratoplasty was performed 4 months later. The patient has remained almost entirely symptom free, and the graft remained clear one year after surgery.ConclusionsA keratitis caused by Pasteurella canis that is resistant to medical therapy can be treated successfully with a conjunctival flap and cryotherapy followed by a penetrating keratoplasty.

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“…canis biotype 1 is mainly observed in the oral cavities of dogs and is also often isolated from the wound sites of humans after receiving dog bites [1], whereas biotype 2 isolates are mainly recovered from calves. There are several reports of P. canis-infected humans with bacteremia [4], soft tissue infection [5], eye infection [6], respiratory infection [7], septic arthritis [8], osteomyelitis [9], gastrointestinal infection [10], breast implant infection [11], and peritonitis [12]. The Emergency Medicine Animal Bite Infection Study Group [13] performed a bacteriological assay of infected wound sites resulting from dog and cat bites, showing that P. canis was the most common species isolated from wounds caused by dog bites, whereas P. multocida subspp.…”

Section: Introductionmentioning

confidence: 99%

Virulence-associated Genome Sequences of Pasteurella canis and Unique Toxin Gene Prevalence of P. canis and Pasteurella multocida Isolated from Humans and Companion Animals

et al. 2022

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Background: Comparative analysis of virulence factors (VFs) between Pasteurella canis and Pasteurella multocida are lacking, although both cause zoonotic infections. We determined the virulence-associated genome sequence characteristics of P. canis and assessed the toxin gene prevalence unique to P. canis among clinical isolates of P. canis and P. multocida.Methods: We selected 10 P. canis and 16 P. multocida whole-genome sequences (WGSs) from the National Center for Biotechnology database. The VFanalyzer tool was used to estimate P. canis-characteristic VFs. Amino acid sequences of VFs were compared with multiple-aligned sequences. The genome structure containing P. canis-characteristic and adjacent loci was compared to the corresponding P. multocida genome structure. After designing primer sequences and assessing their accuracy, we examined the gene prevalence of the P. canis-characteristic VFs using PCR among clinical isolates of P. multocida and P. canis.Results: Using VFanalyzer, we found virulence-associated cytolethal distending toxin (cdt) A-cdtB-cdtC loci common to all P. canis WGSs that were not found in P. multocida WGSs. Similarities in the multiple alignments of CdtA-CdtB-CdtC amino acid sequences were found among the 10 P. canis WGSs. Shared or similar loci around cdtA-cdtB-cdtC were identified between the P. canis and P. multocida genome structures. The PCR-based cdtA-cdtB-cdtC prevalence differed for P. canis and P. multocida clinical isolates.Conclusions: P. canis-specific cdtA-cdtB-cdtC prevalence was identified among clinical isolates. These three loci may be unique toxin genes and promising targets for the rapid identification of P. canis in clinical settings.

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Identification of Pasteurella canis in a Soft Tissue Infection Caused by a Dog Bite: The First Report in Korea

Cited by 11 publications

References 10 publications

Characterization, mechanism of action and optimization of activity of a novel peptide-peptoid hybrid against bacterial pathogens involved in canine skin infections

Characterization, mechanism of action and optimization of activity of a novel peptide-peptoid hybrid against bacterial pathogens involved in canine skin infections

Medical and surgical management of Pasteurella canis infectious keratitis

Virulence-associated Genome Sequences of Pasteurella canis and Unique Toxin Gene Prevalence of P. canis and Pasteurella multocida Isolated from Humans and Companion Animals

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