Identification of n -Decane Oxidation Products in Corynebacterium Cultures by Combined Gas Chromatography-Mass Spectrometry

Abstract

The gas chromatography-mass spectrometry technique was employed to characterize n -decane oxidation products of Corynebacterium strains 7E1C and 269 (SNAM Progetti collection) after 73 h of incubation at 35 C. Corynebacterium 7E1C accumulated consistent amounts of esters of long chain acids with long chain alcohols, mainly decyldecanoate as well as products with mono- and diterminal carboxylic functions. Corynebacterium 269 yielded 1-decanol and 1-10 decanediol as principal oxidation products.

Characterization of Acyltransferases in Neutral Lipid Synthesis

“…WE as storage compounds were detected in several genera (Wältermann & Steinbuchel, 2005b) e.g. in Acinetobacter (Gallagher, 1971;Fixter & Fewson, 1974;Fixter & McCormack, 1976;Scott & Finnerty, 1976), Moraxella (Bryn et al, 1977), Micrococcus (Russell & Volkman, 1980), Fundibacter (Bredemeier et al, 2003), Corynebacterium (Bacchin et al, 1974), Mycobacterium (Wang et al, 1972), Nocardia (Raymond & Davis, 1960) and Marinobacter (Rontani et al, 1999;Holtzapple & Schmidt-Dannert, 2007). Whereas WE in plants are mostly synthesized and deposited to build a protection barrier or to form a carbon reservoir for the development of the next generation, bacteria store WE under environmentally unfavorable conditions.…”