Identification of
            <i>Mycoplasma mycoides</i>
            subsp.
            <i>mycoides</i>
            Small Colony Genes Coding for T-Cell Antigens

Totté, Philippe; Mather, Arshad; Reslan, Lina; Boublik, Yan; Niang, Mamadou; Plessis, D.H. Du; Dedieu, Laurence

doi:10.1128/cvi.00132-10

Cited by 3 publications

(3 citation statements)

References 22 publications

(34 reference statements)

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“…The relevance of the level of CD44 expression by memory T cells is controversial for both mice [ 15 , 69 , 70 ] and humans [ 66 , 69 ]. Although few authors investigated T cell memory responses in cattle [ 24 , 26 , 41 , 71 – 73 ], to our knowledge no other description of the expression of CD44 by bovine Tcm cells (as defined by CCR7 and CD45RO expression) has been published, making direct comparisons not possible. However, data from mice suggest that although the expression of CD44 is dispensable for early expansion, trafficking and cytokine production of Th1 cells; expression of CD44 is required for long-term cell survival and anamnestic responses to re-infection [ 70 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Effector and Memory T Cell Subsets in the Immune Response to Bovine Tuberculosis in Cattle

et al. 2015

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Cultured IFN-γ ELISPOT assays are primarily a measure of central memory T cell (Tcm) responses with humans; however, this important subset of lymphocytes is poorly characterized in cattle. Vaccine-elicited cultured IFN-γ ELISPOT responses correlate with protection against bovine tuberculosis in cattle. However, whether this assay measures cattle Tcm responses or not is uncertain. The objective of the present study was to characterize the relative contribution of Tcm (CCR7+, CD62Lhi, CD45RO+), T effector memory (Tem, defined as: CCR7-, CD62Llow/int, CD45RO+), and T effector cells (CCR7-, CD62L-/low, CD45RO-), in the immune response to Mycobacterium bovis. Peripheral blood mononuclear cells (PBMC) from infected cattle were stimulated with a cocktail of M. bovis purified protein derivative, rTb10.4 and rAg85A for 13 days with periodic addition of fresh media and rIL-2. On day 13, cultured PBMC were re-stimulated with medium alone, rESAT-6:CFP10 or PPDb with fresh autologous adherent cells for antigen presentation. Cultured cells (13 days) or fresh PBMCs (ex vivo response) from the same calves were analyzed for IFN-γ production, proliferation, and CD4, CD45RO, CD62L, CD44, and CCR7 expression via flow cytometry after overnight stimulation. In response to mycobacterial antigens, ~75% of CD4+ IFN-γ+ cells in long-term cultures expressed a Tcm phenotype while less than 10% of the ex vivo response consisted of Tcm cells. Upon re-exposure to antigen, long-term cultured cells were highly proliferative, a distinctive characteristic of Tcm, and the predominant phenotype within the long-term cultures switched from Tcm to Tem. These findings suggest that proliferative responses of Tcm cells to some extent occurs simultaneously with reversion to effector phenotypes (mostly Tem). The present study characterizes Tcm cells of cattle and their participation in the response to M. bovis infection.

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Section: Discussionmentioning

confidence: 99%

Characterization of Effector and Memory T Cell Subsets in the Immune Response to Bovine Tuberculosis in Cattle

et al. 2015

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“…Because lipoproteins are known to stimulate the release of proinflamatory cytokines and are strongly immunodominant, they are considered to be prime target antigens for the development of specific and sensitive serological diagnostic tests. LppA is a highly conserved antigen of Mmm capable of stimulating both humoral and cell mediated response and as such is a potential candidate for a future CBPP vaccine [45][46][47]. LppB is expressed in African and Australian but not in European clusters [10,29].…”

Section: The Membrane Is the Primary Point Of Contact Betweenmentioning

confidence: 99%

“…For instance, LppA (MSC_0013), and prolipoproteins MSC_0635 and MSC_0653 were detected only in strain N6-(highly pathogenic) as was an additional hypothetical predicted membrane protein (MSC_0065). Of these (pro)lipoproteins, only LppA has been investigated to any extent and reported as an immunogen as mentioned above [46,47]. One could assume that these differences may correlate with differences in pathogenicity between the two strains, although no role in virulence has been defined for LppA and no information is available on the other three proteins.…”

Section: Comparison Of the Proteomes Of MMM N6 And Kh3j Strainsmentioning

confidence: 99%

Proteomic Characterisation of Two Strains of Mycoplasma mycoides Subsp. Mycoides of Differing Pathogenicity

Krasteva¹,

Inglis²,

Sacchini³

et al. 2015

J Proteomics Bioinform

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Mycoplasmas are no exception to extracellular vesicles release: Revisiting old concepts

et al. 2018

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Release of extracellular vesicles (EV) by Gram-negative and positive bacteria is being frequently reported. EV are nano-sized, membrane-derived, non-self-replicating, spherical structures shed into the extracellular environment that could play a role in bacteria-host interactions. Evidence of EV production in bacteria belonging to the class Mollicutes, which are wall-less, is mainly restricted to the genus Acholeplasma and is scanty for the Mycoplasma genus that comprises major human and animal pathogens. Here EV release by six Mycoplasma (sub)species of clinical importance was investigated. EV were obtained under nutritional stress conditions, purified by ultracentrifugation and observed by electron microscopy. The membrane proteins of EV from three different species were further identified by mass spectrometry as a preliminary approach to determining their potential role in host-pathogen interactions. EV were shown to be released by all six (sub)species although their quantities and sizes (30–220 nm) were very variable. EV purification was complicated by the minute size of viable mycoplasmal cells. The proteins of EV-membranes from three (sub)species included major components of host-pathogen interactions, suggesting that EV could contribute to make the host-pathogen interplay more complex. The process behind EV release has yet to be deciphered, although several observations demonstrated their active release from the plasma membrane of living cells. This work shed new light on old concepts of “elementary bodies” and “not-cell bound antigens”.

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Identification of Mycoplasma mycoides subsp. mycoides Small Colony Genes Coding for T-Cell Antigens

Abstract: Genes of the

Cited by 3 publications

References 22 publications

Characterization of Effector and Memory T Cell Subsets in the Immune Response to Bovine Tuberculosis in Cattle

Characterization of Effector and Memory T Cell Subsets in the Immune Response to Bovine Tuberculosis in Cattle

Proteomic Characterisation of Two Strains of Mycoplasma mycoides Subsp. Mycoides of Differing Pathogenicity

Mycoplasmas are no exception to extracellular vesicles release: Revisiting old concepts

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