Identification of <i>miR-200a</i> as a novel suppressor of connexin 43 in breast cancer cells

Jia, Ming; Zhou, Yan; Du, Junze; Fan, Shenghao; Pan, Beibei; Wang, Yinhuan; Fan, Lingjun; Jiang, Jun

doi:10.1042/bsr20150153

Cited by 20 publications

(18 citation statements)

References 26 publications

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“…Cx43 is reported to be a contributor to normal cell migration [ 8 ] and tumour cell invasion [ 9 ]. We [ 10 ] and others [ 11 ] also claim that Cx43 induces migration and invasion of breast cancer cells. Regulation of Cx43 expression provides promising strategies in regulating cell functions [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 87%

“…According to previous studies, Sp1 (specificity protein 1), Sp3, AP-1 (activating protein 1) and c-Jun can bind directly to the promoter region of Cx43 so as to promote transcription activity [ 12 , 13 ]. In addition, Cx43 is also intimately regulated by miRNAs at the post-transcription level [ 10 , 11 , 14 ]. miRNAs are one of the largest groups of post-transcriptional regulators [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…We and others have scanned and identified miR-1 , miR-206 , miR-200a , miR-381 , miR-23a / b and miR-186 as potent suppressors of Cx43 [ 10 , 11 , 22 ]. However, the mechanism of miR-381 -reduced Cx43 expression was still not revealed.…”

Section: Introductionmentioning

confidence: 99%

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miR-381 suppresses C/EBPα-dependent Cx43 expression in breast cancer cells

Jia

Zhou

et al. 2015

Bioscience Reports

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Section: Introductionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

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miR-381 suppresses C/EBPα-dependent Cx43 expression in breast cancer cells

Jia

Zhou

et al. 2015

Bioscience Reports

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“…miR-206 which targets Cx43-3′UTR strongly decreased Cx43 expression in MCF-7 cells [83]. The association between miRNAs and Cx43 in the regulation of breast cancer was further supported by an evidence where another miRNA, miR-200a, was identified as a novel suppressor of connexin43 in breast cancer cells [84]. Decreased levels of miR-200a were found to be associated with elevated expression of Cx43 in the metastatic breast cancer tissues compared with the primary tumors [84].…”

Section: Connexin and Other Signaling Pathwaysmentioning

confidence: 99%

Connexin’s Connection in Breast Cancer Growth and Progression

Banerjee

2016

International Journal of Cell Biology

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Gap junctions are cell-to-cell junctions that are located in the basolateral surface of two adjoining cells. A gap junction channel is composed of a family of proteins called connexins. Gap junction channels maintain intercellular communication between two cells through the exchange of ions, small metabolites, and electrical signals. Gap junction channels or connexins are widespread in terms of their expression and function in maintaining the development, differentiation, and homeostasis of vertebrate tissues. Gap junction connexins play a major role in maintaining intercellular communication among different cell types of normal mammary gland for proper development and homeostasis. Connexins have also been implicated in the pathogenesis of breast cancer. Differential expression pattern of connexins and their gap junction dependent or independent functions provide pivotal cross talk of breast tumor cells with the surrounding stromal cell in the microenvironment. Substantial research from the last 20 years has accumulated ample evidences that allow us a better understanding of the roles that connexins play in the tumorigenesis of primary breast tumor and its metastatic progression. This review will summarize the knowledge about the connexins and gap junction activities in breast cancer highlighting the differential expression and functional dynamics of connexins in the pathogenesis of the disease.

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“…MicroRNA-200a (miR-200a), a member of the miR-200 family, has been reported to be dysregulated and function as a tumor inhibitor in the pathogenesis of various cancers, such as pancreatic carcinoma11, renal cell carcinoma12, nasopharyngeal carcinoma13, breast cancer14, ovarian cancer15and so on, including HCC. However, the exact role and molecular mechanism of miR-200a in liver cancer have not been elucidated yet.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-200a inhibits cell growth and metastasis by targeting Foxa2 in hepatocellular carcinoma

Chen¹,

Ma²,

Chen³

et al. 2017

J. Cancer

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Background: MicroRNAs (miRNAs) are a class of endogenous, small non-coding RNAs which function as essential posttranscriptional modulators of gene expression tightly involved in a wide range of diseases, including the hepatocellular carcinoma (HCC). Here, the present study was designed to investigate the expression levels and cellular roles of miR-200a in HCC.Methods: Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was used to detect the expression levels of miR-200a in serums and cell lines. Bioinformation analysis, the luciferase reporter assay, qRT-PCR and western blotting were employed to validate Foxa2 as a direct target gene of miR-200a. Cell proliferation, migration and invasion were assessed to identify whether miR-200a could regulate the biological behaviors of HCC cells by targeting Foxa2.Results: In this study, a low level of miR-200a was observed in patients' serums and HCC cell lines. Overexpression of miR-200a in HCC cell lines reduced cell proliferation, migration and invasion. In addition, transcription factor forkhead box A2 (Foxa2) was identified as a novel target of miR-200a and downregulated at mRNA and protein levels in miR-200a overexpressed cells. Meanwhile, restoration of Foxa2 significantly reversed the tumor suppressive effects of miR-200a.Conclusions: These findings indicate that miR-200a regulates the proliferation, migration and invasion of HCC cells by targeting Foxa2, suggesting that miR-200a may function as a potential therapeutic molecular for the diagnosis and treatment of the liver cancer.

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Identification of miR-200a as a novel suppressor of connexin 43 in breast cancer cells

Cited by 20 publications

References 26 publications

miR-381 suppresses C/EBPα-dependent Cx43 expression in breast cancer cells

miR-381 suppresses C/EBPα-dependent Cx43 expression in breast cancer cells

Connexin’s Connection in Breast Cancer Growth and Progression

MicroRNA-200a inhibits cell growth and metastasis by targeting Foxa2 in hepatocellular carcinoma

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