Identification of <i>Malassezia</i> species in the facial lesions of Chinese seborrhoeic dermatitis patients based on DNA sequencing

Lian, Cuihong; Shen, Liliang; Gao, Qingyu; Jiang, Ming; Zhao, Zijun; Zhao, Jing

doi:10.1111/myc.12229

Cited by 15 publications

(13 citation statements)

References 25 publications

(39 reference statements)

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“…Findings regarding M. furfur were mostly compatible to those of other studies carried out in Japan [ 28 ], Korea [ 29 ], China [ 30 ], Sweden [ 31 ], and Canada [ 32 ]. DNA sequence analysis is a rapid, stable, and effective method for the identification and strain typing of Malassezia species [ 30 , 33 ]. In the present study, the use of universal primers for the amplification of ITS regions led to the separation of non- Malassezia species and Malassezia species.…”

Section: Discussionsupporting

confidence: 87%

In vitro activity of kombucha tea ethyl acetate fraction against Malassezia species isolated from seborrhoeic dermatitis

Mahmoudi

Saeidi

et al. 2016

mazu-cmm

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Background and Purpose:Seborrheic dermatitis is a chronic and recurrent superficial dermatitis in which Malassezia species play an important role. There are different Malassezia species, which have been recently reported to be resistant to common antifungals. Natural sources can be useful alternatives to reduce the emergence of this resistance. Kombucha tea is believed to have potential antimicrobial properties. Regarding this, the present study aimed to investigate the antifungal activity of Kombucha tea ethyl acetate fraction (KEAF) against Malassezia species obtained from the patients with seborrheic dermatitis. Materials and Methods:A total of 23 clinical isolates were identified by direct microscopic examination and Tween assimilation, and then confirmed by DNA sequencing of ITS regions for Malassezia species. Kombucha tea was fractionated using ethyl acetate (1:2 v/v). The minimum inhibitory concentration (MIC) microdilution assay was used to evaluate the anti-Malssezia activity of KEAF at three concentrations of 10, 40, and 80 mg/mL. Results:The results of the DNA sequence analysis indicated that M. furfur (39.13%) was the predominant species, followed by M. globosa (30.43%), M. sloofie (13.04%), M. sympodialis (13.04%), and M. restricta (4.34%), respectively. Furthermore, KEAF showed inhibitory activity against Malassezia species. Accordingly, KEAF had the lowest and highest MIC value against M. sloofie and M. restricta, respectively. Moreover, the inhibitory effect of the extract was equivalent to that of ketoconazole at 4.8 µg/mL. Conclusion:The findings of the current study highlighted the antifungal properties of KEAF. Therefore, this extract can be promoted as complementary medicine for the treatment of the infections caused by Malassezia.

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Section: Discussionsupporting

confidence: 87%

In vitro activity of kombucha tea ethyl acetate fraction against Malassezia species isolated from seborrhoeic dermatitis

Mahmoudi

Saeidi

et al. 2016

mazu-cmm

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“…[18] A recent study of Chinese patients with SD showed no significant difference in the distribution of Malassezia species between Chinese SD patients and healthy individuals. [19] In the present study, we examined the presence of Malassezia by conventional KOH examination and fungal culture, and unexpectedly, found only two positive findings in 37 SD patients (data not shown). We speculate that factors such as the relatively cold temperature and low humidity at the time of the study, or the ethnicity of the cohort, might have affected the detection of Malassezia , and we suggest a less essential role of Malassezia in the pathogenesis of SD.…”

Section: Discussionmentioning

confidence: 57%

High Staphylococcus epidermidis Colonization and Impaired Permeability Barrier in Facial Seborrheic Dermatitis

Sun

et al. 2017

Chinese Medical Journal

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Background:Seborrheic dermatitis (SD) is a common inflammatory skin condition. The etiology is unclear, although overgrowth of Malassezia on the skin has been suggested to cause SD. This study investigated whether colonization with Staphylococcus plays a role in facial SD, which was not well addressed previously.Methods:The study was conducted from September 1, 2011 to February 20, 2012 in the First Hospital of China Medical University. In the first phase, the study evaluated the level of transepidermal water loss (TEWL) and the number of colony-forming units (CFU) of Staphylococcus in defined skin areas of SD patients who were human immunodeficiency virus (HIV) seropositive (HIV [+] SD [+] group, n = 13), classical SD (HIV [−] SD [+] group, n = 24) patients, HIV seropositive-non-SD (HIV [+] SD [−] group, n = 16) patients, and healthy volunteers (HIV [−] SD [−] group, n = 16). In the second phase, we enrolled another cohort of HIV (−) SD (+) patients who applied topical fusidic acid (n = 15), tacrolimus (n = 16), or moisturizer (n = 12). Changes in the Seborrheic Dermatitis Area Severity Index (SDASI), TEWL, and Staphylococcus density were evaluated 2 weeks later. Comparisons of each index were performed using analysis of variance (ANOVA) and least significant difference method.Results:The level of TEWL was greater through lesional sites in the HIV (+) SD (+) group than that in HIV (+) SD (−) and HIV (−) SD (−) groups (95% confidence interval [CI]: 18.873–47.071, P < 0.001 and 95% CI: 28.755–55.936, P < 0.001, respectively). The number of CFU of Staphylococcus was greater in the HIV (+) SD (+) group than that in HIV (+) SD (−) and HIV (−) SD (−) groups (95% CI: 37.487–142.744, P = 0.001 and 95% CI: 54.936–156.400, P < 0.001, respectively). TEWL was significantly more improved in patients treated with tacrolimus and fusidic acid than that in those treated with moisturizers (95% CI: 7.560–38.987, P = 0.004 and 95% CI: 4.659–37.619, P = 0.011, respectively). Topical tacrolimus and fusidic acid were significantly associated with decreased SDASI as compared with moisturizer (95% CI: 0.03–0.432, P = 0.025 and 95% CI: 0.033–0.44, P = 0.024, respectively).Conclusions:High colonization with Staphylococcus epidermidis, along with impaired skin permeability barrier function, contributes to the occurrence of SD.

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“…Although stripping of the skin explants caused an inflammatory response that could potentially interfere with the testing of topical products, skin viability and cytokine levels had returned to their basal levels within 48 h, suggesting that skin homeostasis had been restored by this time. Although several species of the Malassezia genus have been associated with SEBD, M. furfur has been shown to be one of the predominant species in SEBD [16, 17] and thus was chosen to generate this model. Future models using different Malassezia species or even clinical isolates would further extend its suitability.…”

Section: Discussionmentioning

confidence: 99%

Novel Non-Steroidal Facial Cream Demonstrates Antifungal and Anti-Inflammatory Properties in Ex Vivo Model for Seborrheic Dermatitis

Granger

Balato

Goñi‐de‐Cerio

et al. 2019

Dermatol Ther (Heidelb)

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Introduction Seborrheic dermatitis (SEBD) is a chronic, recurrent skin disorder that typically occurs as an inflammatory response to fungi of the genus Malassezia . The development of an ex vivo model that mimics the fungal proliferation and skin inflammation of SEBD would play an important role in screening formulations for their efficacy in treating SEBD. Methods An ex vivo model for SEBD using human skin explants that had been mechanically manipulated to facilitate colonization of Malassezia furfur was developed. This model was used to evaluate the efficacy of a novel non-steroidal facial cream (NSFC) in inhibiting M. furfur proliferation and reducing inflammatory cytokine levels. Results This model reproduced some of the key pathological features of SEBD, including M. furfur proliferation and inflammatory cytokine production. Topical application of NSFC facial cream reduced M. furfur counts by 92% ( p < 0.05) and levels of interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α) by 82% and 40%, respectively ( p < 0.05, both). Conclusion The proposed ex vivo model for SEBD could be a useful tool to evaluate topical antifungal treatments. The novel NSFC tested in this study reduced M. furfur proliferation and inflammatory cytokine levels following topical application and may be helpful in the management of SEBD. Funding ISDIN. Electronic supplementary material The online version of this article (10.1007/s13555-019-0311-4) contains supplementary material, which is available to authorized users.

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Identification of Malassezia species in the facial lesions of Chinese seborrhoeic dermatitis patients based on DNA sequencing

Cited by 15 publications

References 25 publications

In vitro activity of kombucha tea ethyl acetate fraction against Malassezia species isolated from seborrhoeic dermatitis

In vitro activity of kombucha tea ethyl acetate fraction against Malassezia species isolated from seborrhoeic dermatitis

High Staphylococcus epidermidis Colonization and Impaired Permeability Barrier in Facial Seborrheic Dermatitis

Novel Non-Steroidal Facial Cream Demonstrates Antifungal and Anti-Inflammatory Properties in Ex Vivo Model for Seborrheic Dermatitis

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