Identification of <i>Cryptosporidium parvum</i> ‘cattle’ genotype from a severe outbreak of neonatal foal diarrhoea

Grinberg, Alex; Oliver, Lyda; Learmonth, J. J.; Leyland, M. J.; Roe, Wendi D.; Pomroy, W.E.

doi:10.1136/vr.153.20.628

Cited by 44 publications

(26 citation statements)

References 23 publications

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“…hominis, C. tyzzeri, C. felis, C. ubiquitum and C. andersoni (Grinberg et al, 2003;Xiao, 2010;Guo et al, 2014;Liu et al, 2015;Qi et al, 2015;Jian et al, 2016;Wagnerová et al, 2016). In the present study, only 293bp of clean sequence for the 18S locus was obtained and therefore further studies on a larger number of isolates at multiple loci are required to fully characterize the novel species from horses.…”

Section: Discussionmentioning

confidence: 72%

Genetic characterization of Cryptosporidium in animal and human isolates from Jordan

Hijjawi

Mukbel

Yang

et al. 2016

Veterinary Parasitology

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(Mukbel R). #Joint first authors Highlights First genotyping study of Cryptosporidium in animals in Jordan  Faecal samples (n=284) from domestic animals and humans (n=48) screened  Overall prevalence of 11.6% in animals and six species detected.  Novel Cryptosporidium genotype detected in horses AbstractLittle is known about the epidemiology of Cryptosporidium in Jordan and to date, only one genotyping study has been conducted on Cryptosporidium isolates from Jordanian children. In the present study, a total of 284 faecal samples from Jordanian cattle, sheep, goats and chicken and 48 human faecal samples were screened for the presence of Cryptosporidium using an 18S quantitative PCR (qPCR) and a C. parvum/C. hominis specific qPCR at a lectin locus. Of these, 37 of 284 animal faecal samples were positive by qPCR at the 18S locus giving an overall prevalence of 11.6%. The point prevalence of Cryptosporidium in chickens, sheep, horses, cattle and goats ranged from 4.8% (chickens) to 18.7% (cattle). A total of six species were detected; C. xiaoi (n=9), C.andersoni (n=7), C. ryanae (n=5), C. parvum (n=4), C. baileyi (n=1) and a genetically distinct and potentially novel species in two isolates from horses. Sub-genotype analysis of the 4 C. parvum isolates at the 60-kDa glycoprotein (gp60) locus identified subtype IIaA19G2R1 (n=2) and IIaA16GR1 (n=2). For the human samples, 4 positives (8.3% prevalence) were detected. Of these, two were C. parvum (subtypes IIdA20G1 and IIaA15G2R1) and two were C. hominis (subtypes 1bA9G3 and 1bA10G2R2). Further studies are required to better understand the epidemiology and transmission of Cryptosporidium in Jordan.

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Section: Discussionmentioning

confidence: 72%

Genetic characterization of Cryptosporidium in animal and human isolates from Jordan

Hijjawi

Mukbel

Yang

et al. 2016

Veterinary Parasitology

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“…The variability in the primer sequences is the probable explanation for the failure in amplifying DNA. Though, in different regions of the world, Cryptosporidium infections have been reported in goats, rabbits (Marlier et al, 2003;Peeters, 1988;Shiibashi et al, 2006), horses (Browning et al, 1991;Darabus et al, 2001;Grinberg et al, 2003;Majewska et al, 1999;Majewsk et al, 2004;Olson et al, 1997), all examined animals in these species in Tunisia were not found to be infected with Cryptosporidium. The age of sampled animals as well as animal management practices or environmental conditions could explain such results.…”

Section: Discussionmentioning

confidence: 99%

Prevalence ofCryptosporidiumspp. (Eucoccidiorida: Cryptosporiidae) in seven species of farm animals in Tunisia

Soltane¹,

Guyot

Dei‐Cas

et al. 2007

Parasite

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Summary :1,001 faecal samples were obtained from 89 sheep (lambs and adult), 184 goats, 190 horses, 178 rabbits, 110 camels, 200 broiler chicken and 50 turkeys housed in farms from different localities in Tunisia. All samples were analysed for Cryptosporidium oocysts by microscopic examination of smears stained by modified Ziehl Neelsen technique. The parasite was detected in ten lambs and adult sheep (11.2 %) and nine broiler chicken (4.5 %). Molecular characterization, performed in four animals, identified C. bovis in three lambs and C. meleagridis in one broiler chicken. This work is the first report on Cryptosporidium in farm animals in Tunisia. Résumé

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“…Cryptosporidium is recognized as one of the main causes of diarrhea in lambs (de GRAAF et al, 1999), causing high morbidity and mortality and major economic losses (CASEMORE et al, 1997). In pigs and equines Cryptosporidium appear to be of less zoonotic importance, although diarrhea in post-weaning piglets and reduction in growth rate, food absorption and weight loss in equine have already been described (MISIC et al, 2003;GRINBERG et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Occurrence and molecular characterization of Cryptosporidium spp. isolated from domestic animals in a rural area surrounding Atlantic dry forest fragments in Teodoro Sampaio municipality, State of São Paulo, Brazil

Sevá

Funada

Souza

et al. 2010

Rev. Bras. Parasitol. Vet.

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The aim of this study was to assess the occurrence of Cryptosporidium in domestic animals in rural properties surrounding rain forest fragments within the municipality of Teodoro Sampaio, southeastern Brazil. Conventional sucrose flotation method followed by molecular characterization of the parasites by sequencing PCR products amplified from SSU rRNA gene were used. Stool samples were collected from domestic animals raised as pets and livestock in all rural properties surrounding three forest fragments. Samples from cattle (197), equine (63), pigs (25), sheep (11), and dogs (28) were collected from 98 rural properties. The frequency of occurrence of Cryptosporidium within each animal species was 3.0% (6/197) among cattle and 10.7% (3/28) among dogs. Cryptosporidium was not detected in stool samples from equine, sheep, and pigs. All sequences obtained from the six samples of calves showed molecular identity with Cryptosporidium andersoni while all sequences from dog samples were similar to C. canis. The frequency of occurrence of Cryptosporidium in these domestic animal species was low. The absence of C. parvum in the present study suggests that the zoonotic cycle of cryptosporidiosis may not be relevant in the region studied. The presence of Cryptosporidium species seldom described in humans may be, otherwise, important for the wild fauna as these animals are a source of infection and dissemination of this protozoan to other animal species. The impact and magnitude of infection by C. andersoni in wild ruminants and C. canis in wild canids have to be assessed in future studies to better understand the actual importance of these species in this region.

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Identification of Cryptosporidium parvum ‘cattle’ genotype from a severe outbreak of neonatal foal diarrhoea

Cited by 44 publications

References 23 publications

Genetic characterization of Cryptosporidium in animal and human isolates from Jordan

Genetic characterization of Cryptosporidium in animal and human isolates from Jordan

Prevalence ofCryptosporidiumspp. (Eucoccidiorida: Cryptosporiidae) in seven species of farm animals in Tunisia

Occurrence and molecular characterization of Cryptosporidium spp. isolated from domestic animals in a rural area surrounding Atlantic dry forest fragments in Teodoro Sampaio municipality, State of São Paulo, Brazil

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