Identification of <i>cis</i> elements involved in Commelina yellow mottle virus promoter activity

Medberry, Scott L.; Olszewski, Neil E.

doi:10.1046/j.1365-313x.1993.03040619.x

Cited by 30 publications

(15 citation statements)

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“…5A; AVP1-1 background). We used the well-characterized promoter element from Commelina Yellow Mottle Virus (pCoYMV) that directs expression specifically in the companion cells (Medberry and Olszewski, 1993;Dasgupta et al, 2014). All three independent pCoYMV::AVP1 lines showed a Figure 7.…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis Type I Proton-Pumping Pyrophosphatase Expresses Strongly in Phloem, Where It Is Required for Pyrophosphate Metabolism and Photosynthate Partitioning

Pizzio

Páez-Valencia²,

Khadilkar³

et al. 2015

Plant Physiol.

105

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Phloem loading is a critical process in plant physiology. The potential of regulating the translocation of photoassimilates from source to sink tissues represents an opportunity to increase crop yield. Pyrophosphate homeostasis is crucial for normal phloem function in apoplasmic loaders. The involvement of Arabidopsis (Arabidopsis thaliana) type I proton-pumping pyrophosphatase (AVP1) in phloem loading was analyzed at genetic, histochemical, and physiological levels. A transcriptional AVP1 promoter:: GUS fusion revealed phloem activity in source leaves. Ubiquitous AVP1 overexpression (35S::AVP1 cassette) enhanced shoot biomass, photoassimilate production and transport, rhizosphere acidification, and expression of sugar-induced root ion transporter genes (POTASSIUM TRANSPORTER2 By contrast, phloem-specific AVP1 knockdown (pCoYMV:: RNAi AVP1) resulted in stunted seedlings in sucrose-deprived medium. We also present a promoter mutant avp1-2 (SALK046492) with a 70% reduction of expression that did not show severe growth impairment. Interestingly, AVP1 protein in this mutant is prominent in the phloem. Moreover, expression of an Escherichia colisoluble pyrophosphatase in the phloem (pCoYMV::pyrophosphatase) of avp1-2 plants resulted in severe dwarf phenotype and abnormal leaf morphology. We conclude that the Proton-Pumping Pyrophosphatase AVP1 localized at the plasma membrane of the sieve element-companion cell complexes functions as a synthase, and that this activity is critical for the maintenance of pyrophosphate homeostasis required for phloem function.[

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Section: Discussionmentioning

confidence: 99%

Arabidopsis Type I Proton-Pumping Pyrophosphatase Expresses Strongly in Phloem, Where It Is Required for Pyrophosphate Metabolism and Photosynthate Partitioning

Pizzio

Páez-Valencia²,

Khadilkar³

et al. 2015

Plant Physiol.

105

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“…This result indicates that there may be similar transcription factors in tobacco and rice that regulate expression of the promoter. It is known that some plant promoters retain specific expression patterns in different plant species (21)(22)(23)(24)(26)(27)(28)(29)(30)(31) while others do not (32,33).…”

Section: Discussionmentioning

confidence: 99%

Transcription factor RF2a alters expression of the rice tungro bacilliform virus promoter in transgenic tobacco plants

Petruccelli

Dai

Carcamo

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

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The promoter from rice tungro bacilliform badnavirus (RTBV) is expressed only in phloem tissues in transgenic rice plants. RF2a, a b-Zip protein from rice, is known to bind to the Box II cis element near the TATA box of the promoter. Here, we report that the full-length RTBV promoter and a truncated fragment E of the promoter, comprising nucleotides ؊164 to ؉45, result in phloemspecific expression of ␤-glucuronidase (GUS) reporter genes in transgenic tobacco plants. When a fusion gene comprising the cauliflower mosaic virus 35S promoter and RF2a cDNA was coexpressed with the GUS reporter genes, GUS activity was increased by 2-20-fold. The increase in GUS activity was positively correlated with the amount of RF2a, and the expression pattern of the RTBV promoter was altered from phloem-specific to constitutive. Constitutive expression of RF2a did not induce morphological changes in the transgenic plants. In contrast, constitutive overexpression of the b-ZIP domain of RF2a had a strong effect on the development of transgenic plants. These studies suggest that expression of the b-Zip domain can interfere with the function of homologues of RF2a that regulate development of tobacco plants. R egulation of transcription is achieved by the activity of multiple proteins that bind to regulatory elements, many of which are upstream of the promoters and alter basal rates of transcription initiation and͞or elongation (1, 2). To understand the mechanisms of tissue-specific and constitutive gene expression in plants, a number of promoters and transcription factors have been studied in recent years (3-16). It was shown that constitutive promoters, such as the cauliflower mosaic virus 35S promoter (17) and the promoter from cassava vein mosaic virus (14) are modular in organization and multiple cis elements. These elements, with specific transcription factors, apparently interact in an additive and͞or synergistic manner to confer gene expression in all plant tissues. Similarly, tissue-specific promoters contain multiple elements that contribute to promoter activity in both positive and negative ways (4-6, 8, 12, 18).The rice tungro bacilliform badnavirus (RTBV) promoter (19,20) and the transcription factors that interact it may serve as a model system to study plant tissue-specific gene expression. The virus, which replicates solely in phloem tissues, has a single promoter that is active in transfected protoplasts and is phloemspecific in transgenic rice plants (7,10,21,22).Within the fragment E of the promoter (nucleotides Ϫ164 to ϩ45), multiple cis elements were identified as being required for phloem-specific gene expression (7,10,15,23). A b-ZIP type transcription factor, RF2a, was isolated from rice and bound to Box II, a crucial cis element of the promoter. RF2a activates transcription from the RTBV promoter in an in vitro transcription system derived from rice cell cultures (11). Moreover, studies in transgenic rice plants suggested that RF2a is involved in the development of vascular tissues (11).We report here the functi...

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“…The transcription start site is designated + 1. Consensus TATA box, as l-like sequence [29] and the region homologous with the CoYMV promoter [35] are boxed. Alul sites @)indicate the 5 / and 3 / ends of the CVP 1 promoter.…”

Section: Determination Of the Transcription Start Sitementioning

confidence: 99%

Isolation and expression in transgenic tobacco and rice plants, of the cassava vein mosaic virus (CVMV) promoter

et al. 1996

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The cassava vein mosaic virus (CVMV) is a double stranded DNA virus which infects cassava plants (Manihot esculenta Crantz) and has been characterized as a plant pararetrovirus belonging to the caulimovirus subgroup. Two DNA fragments, CVP1 of 388 nucleotides from position -368 to +20 and CVP2 of 511 nucleotides from position -443 to +72, were isolated from the viral genome and fused to the uidA reporter gene to test promoter expression. The transcription start site of the viral promoter was determined using RNA isolated from transgenic plants containing the CVMV promoter:uidA fusion gene. Both promoter fragments were able to cause high levels of gene expression in protoplasts isolated from cassava and tobacco cell suspensions. The expression pattern of the CVMV promoters was analyzed in transgenic tobacco and rice plants, and revealed that the GUS staining pattern was similar for each construct and in both plants. The two promoter fragments were active in all plant organs tested and in a variety of cell types, suggesting a near constitutive pattern of expression. In both tobacco and rice plants, GUS activity was highest in vascular elements, in leaf mesophyll cells, and in root tips.

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Identification of cis elements involved in Commelina yellow mottle virus promoter activity

Cited by 30 publications

References 0 publications

Arabidopsis Type I Proton-Pumping Pyrophosphatase Expresses Strongly in Phloem, Where It Is Required for Pyrophosphate Metabolism and Photosynthate Partitioning

Arabidopsis Type I Proton-Pumping Pyrophosphatase Expresses Strongly in Phloem, Where It Is Required for Pyrophosphate Metabolism and Photosynthate Partitioning

Transcription factor RF2a alters expression of the rice tungro bacilliform virus promoter in transgenic tobacco plants

Isolation and expression in transgenic tobacco and rice plants, of the cassava vein mosaic virus (CVMV) promoter

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