Identification of cfxR, an activator gene of autotrophic CO2 fixation in Alcaligenes eutrophus

Windhövel, Ute; Bowien, Botho

doi:10.1111/j.1365-2958.1991.tb01978.x

Cited by 61 publications

(67 citation statements)

References 41 publications

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“…3B, lanes 2 and 3). These values agreed totally with those (33, (40); the X. flavus orfD sequence was partially determined, and the 150-amino-acid portion of the amino terminus was deduced (20). These proteins shared a high level of sequence identity (37.9 to 46.3%) for all regions of the molecule (Fig.…”

Section: Resultssupporting

confidence: 75%

“…In addition to this high level of homology, T. ferrooxidans RbcR possesses the above-described common features, although some The function of this family of proteins requires binding to a specific target sequence(s); i.e., the operator and promoter regions of the genes they regulate. CfxR of A. eutrophus bound to the intergenic region between the cfxL and cfxR genes (40). As expected, RbcR, present in crude extracts from E. coli carrying cloned T. ferrooxidans rbcR, bound specifically to the intergenic sequence between rbcR and rbcLl (Fig.…”

Section: Discussionmentioning

confidence: 59%

“…In A. eutrophus, a cfx operon is located in the chromosome and another is found in a plasmid. A single copy of cfxR, found upstream of the chromosomal cfx operon, regulates the two cfx operons (40).…”

Section: Discussionmentioning

confidence: 99%

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Specific binding of Thiobacillus ferrooxidans RbcR to the intergenic sequence between the rbc operon and the rbcR gene

Kusano

Sugawara

1993

J Bacteriol

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The presence of two sets (rbcL1-rbcS1 and rbcL2-rbcS2) of rbc operons has been demonstrated in Thiobacillus ferrooxidans Fe1 (T. Kusano, T. Takeshima, C. Inoue, and K. Sugawara, J. Bacteriol. 173:7313-7323, 1991). A possible regulatory gene, rbcR, 930 bp long and possibly translated into a 309-amino-acid protein, was found upstream from the rbcL1 gene as a single copy. The gene is located divergently to rbcL1 with a 144-bp intergenic sequence. As in the cases of the Chromatium vinosum RbcR and Alcaligenes eutrophus CfxR, T. ferrooxidans RbcR is thought to be a new member of the LysR family, and these proteins share 46.5 and 42.8% identity, respectively. Gel mobility shift assays showed that T. ferrooxidans RbcR, produced in Escherichia coli, binds specifically to the intergenic sequence between rbcL1 and rbcR. Footprinting and site-directed mutagenesis experiments further demonstrated that RbcR binds to overlapping promoter elements of the rbcR and rbcL1 genes. The above data strongly support the participation of RbcR in regulation of the rbcL1-rbcS1 operon and the rbcR gene in T. ferrooxidans.

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Section: Resultssupporting

confidence: 75%

Section: Discussionmentioning

confidence: 59%

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Specific binding of Thiobacillus ferrooxidans RbcR to the intergenic sequence between the rbc operon and the rbcR gene

Kusano

Sugawara

1993

J Bacteriol

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“…A regulatory gene cbbR, encoding a transcriptional activator, belongs to the chromosomal locus. A plasmid-borne copy of cbbR is present but defective, so the chromosomal copy controls the coordinate expression of both operons 17 . Two substrates supply energy for autotrophic CO 2 fixation.…”

Section: Lithoautotrophic and Organoautotrophic Metabolismmentioning

confidence: 99%

Genome sequence of the bioplastic-producing “Knallgas” bacterium Ralstonia eutropha H16

et al. 2006

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“…Adjacent to the cbbR gene is an ORF oriented divergently from cbbR; from comparison to a recently determined nucleotide sequence of a cbb gene from A. eutrophus (54), this ORF was identified as the pentose-5-phosphate 3-epimerase gene (cbbE). The translated sequence of the ORF is 53% identical to cbbE fromA.…”

Section: G V L P H R M I E M S S N E T I K Qmentioning

confidence: 99%

Complementation analysis and regulation of CO2 fixation gene expression in a ribulose 1,5-bisphosphate carboxylase-oxygenase deletion strain of Rhodospirillum rubrum

Falcone

Tabita

1993

J Bacteriol

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A ribulose 1,5-bisphosphate carboxylase-oxygenase (RubisCO) deletion strain ofRhodospirillum rubrum that was incapable of photolithoautotrophic growth was constructed. Photoheterotrophic growth, however, was possible for the R. rubrum RubisCO deletion strain when oxidized carbon compounds such as malate were supplied. The R. rubrum RubisCO-deficient strain was not complemented to photolithoautotrophic growth by various R. rubrum DNA fragments that contain the gene encoding RubisCO, cbbM. When the R. rubrum cbbM deletion strain harbored plasmids containing R. rubrum DNA inserts with at least 2.0 kb preceding the translational start site of the cbbM gene, RubisCO activity and RubisCO antigen were detected. Lack of RubisCO expression was therefore not the cause for the failure to complement the cbbM mutant strain. Interestingly, DNA fragments encoding either of two complete Calvin-Benson-Bassham C02 fixation (cbb) gene operons from Rhodobacter sphaeroides were able to complement the R. rubrum RubisCO deletion strain to photolithoautotrophic growth. The same R. rubrum DNA fragments that failed to complement the R. rubrum cbbM deletion strain successfully complemented the RubisCO deletion strain of R. sphaeroides, pointing to distinct differences in the regulation of metabolism and the genetics of photolithoautotrophic growth in these two organisms. A number of cbb genes were identified by nucleotide sequence analysis of the region upstream of cbbM. Included among these was an open reading frame encoding a cbbR gene showing a high degree of sequence similarity to known lysR-type CO2 fixation transcriptional activator genes. The placement and orientation of the cbbR transcriptional regulator gene in R. rubrum are unique.

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Identification of cfxR, an activator gene of autotrophic CO₂ fixation in Alcaligenes eutrophus

Cited by 61 publications

References 41 publications

Specific binding of Thiobacillus ferrooxidans RbcR to the intergenic sequence between the rbc operon and the rbcR gene

Specific binding of Thiobacillus ferrooxidans RbcR to the intergenic sequence between the rbc operon and the rbcR gene

Genome sequence of the bioplastic-producing “Knallgas” bacterium Ralstonia eutropha H16

Complementation analysis and regulation of CO2 fixation gene expression in a ribulose 1,5-bisphosphate carboxylase-oxygenase deletion strain of Rhodospirillum rubrum

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