Identification of<i>Avramr1</i>from<i>Phytophthora infestans</i>using long read and cDNA pathogen-enrichment sequencing (PenSeq)

Lin, Xiao; Song, Tianqiao; Fairhead, Sebastian; Witek, Kamil; Jouet, Agathe; Jupe, Florian; Witek, Agnieszka; Vleeshouwers, Vivianne G. A. A.; Hein, Ingo; Jones, Jonathan D. G.

doi:10.1101/2020.05.14.095158

Cited by 10 publications

(26 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, we also reported that Rpi-amr3 directly interacts with AVRamr3 and other recognized AVRamr3 homologs from different Phytophthora species. Surprisingly, the direct interaction has not led to accelerated evolution of Avramr3 to evade detection, as we also observed for Rpi-amr1 and AVRamr1 (Lin et al, 2020a;Witek et al, 2021). This could predispose Rpi-amr3 to function in different plant species.…”

Section: Discussionsupporting

confidence: 82%

“…Many RXLR effectors are fast-evolving, multiple-member family proteins with extensive sequence polymorphism, such as the Avr2 and Avrblb2 families (Gilroy et al, 2011;Oliva et al, 2015). To study the sequence polymorphism of Avramr3, seventeen additional Avramr3 homologs from eleven isolates were identified from published databases (KR_1, 3928A, EC1, 6_A1 and US23) (Lee et al, 2020;Lin et al, 2020a) or cloned by PCR (EC1, Katshaar, Pi14538, Pi88069, Pi99183 and Pi99177) (Fig. S1).…”

Section: Avramr3 Encodes a Conserved Rxlr-wy Effector Proteinmentioning

confidence: 99%

“…Many Avirulence (Avr) genes encoding recognized effectors from Phytophthora species have been identified and they are often fast-evolving and lineage-specific molecules (Jiang et al, 2008). Recently, AVRamr1 (PITG_07569), the recognized effector of Rpi-amr1 was identified by a long read and cDNA pathogen enrichment sequencing (PenSeq) approach (Lin et al, 2020a). Surprisingly, AVRamr1 homologs were identified from P. parasitica and P. cactorum genomes and both are recognized by all Rpi-amr1 variants (Witek et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Rpi-amr3 confers resistance to multiple Phytophthora species by recognizing a conserved RXLR effector

Olave-Achury

Heal

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Diverse pathogens from the genus Phytophthora cause disease and reduce yields in many crop plants. Although many Resistance to Phytophthora infestans (Rpi) genes effective against potato late blight have been cloned, few have been cloned against other Phytophthora species. Most Rpi genes encode nucleotide-binding domain, leucine-rich repeat- containing (NLR) proteins, that recognize RXLR effectors. However, whether NLR proteins can recognize RXLR effectors from multiple different Phytophthora pathogens has rarely been investigated. Here, we report the effector AVRamr3 from P. infestans that is recognized by Rpi-amr3 from S. americanum. We show here that AVRamr3 is broadly conserved in many different Phytophthora species, and that recognition of AVRamr3 homologs enables resistance against multiple Phytophthora pathogens, including P. parasitica and P. palmivora. Our findings suggest a novel path to identifying R genes against important plant pathogens.

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Avramr3 Encodes a Conserved Rxlr-wy Effector Proteinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Rpi-amr3 confers resistance to multiple Phytophthora species by recognizing a conserved RXLR effector

Olave-Achury

Heal

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…A new effector gene was identified as Avr-amr1 by HR when it was transiently coexpressed in N. benthamiana with the matching Rpi-amr1. In the T30-4 genome, the newly characterized Avr-amr1 locus was found physically close to two already known Avr effector genes, Avr8 and Avr-Smi-ra1 [83].…”

Section: Phytophthora Infestans: Genome and Virulence Gene Evolutionmentioning

confidence: 81%

Plant–Pathogen Molecular Dialogue: Evolution, Mechanisms and Agricultural Implementation

Хавкин

2021

Russ J Plant Physiol

View full text Add to dashboard Cite

Plant diseases persistently challenge sustainable crop production worldwide. The most economical and eco-friendly way to effectively deal with this problem is to breed new cultivars with stable and durable resistance. Current progress towards this goal has been reinforced by considerable advancements in the molecular studies of pathogens and host plants. These advancements have greatly benefited from recently developed methods to research into gene structure and activity, especially the “omics” technologies. These steps forward are vividly represented by the case of late blight, which is economically the most important disease of potato and tomato (Solanum L.). Late blight became a popular model of multidimensional plant-microbe interactions, and newly obtained molecular evidence has considerably reshaped both our vision of plant–pathogen molecular dialogue and our approach to mitigating this disease. Drawing on recent publications, this review will focus on genome of the causal agent of disease, the oomycete Phytophthora infestans (Mont.) de Bary, and its already characterized genes of virulence, with particular emphasis on their evolution, which underlines the exceptional genetic and phenotypic plasticity of this pathogen. Specially highlighted is the diversity of the immediate tools of virulence—effectors, which interact with potato target molecules, alter host physiology and facilitate plant colonization. Turning to plant defense barriers, the reviewer elaborates on the polymorphism and evolution of Solanum genes providing for plant resistance to P. infestans. The repertoire of P. infestans virulence genes in agrocenoses and the diversity of resistance genes in potato wild relatives are explored as regards the agriculture-oriented implementation of new molecular knowledge. The multifaceted approach to late blight combines the search for new resistance genes in genetic collections, the characterization of their function and stacking these genes in potato cultivars in order to breed new donors of long-lasting and durable resistance together with express assessment of pathogen virulence genes.

show abstract

“…Thus, breeding for resistance against P. capsici implies dealing with at least three sets of genetic determinants in the same plant. In P. infestans and Phytophthora sojae all effectors demonstrated to trigger immunity belong to the RXLR class (Yin et al ., 2017; Zhang et al ., 2019, Lin et al ., 2020), and their plant counterparts, that is, the proteins that recognize them, all belong to the NLR class (Zhang et al ., 2013; Vossen et al ., 2016). It is thus likely that additional gene‐for‐gene interactions between other Phytophthora species and their host plants are dictated by the same types of proteins.…”

Section: Structural Specificity Of Plant Resistance and Susceptibilitymentioning

confidence: 99%

Structural specificity in plant–filamentous pathogen interactions

Lacaze

Joly

2020

Molecular Plant Pathology

View full text Add to dashboard Cite

Phytopathogens often establish and proliferate on specific plant organs and tissues, triggering leaf spots, stem cankers, fruit or root rots, and tuber scabs. A pathogen's structural specificity is often caused by adaptation to a given organ (leaf, stem, flower, root, etc.), tissue (xylem, phloem, mesophyll, etc.), or a developmental stage of the host (Barrett and Heil, 2012). Such specificity can be expressed at the level of its entry tissue, its colonized tissue, its sporulating tissue, or any combination of these factors. Pathogens can exert high structural specificity, such as powdery mildew species confined to the leaf epidermis, or have no structural specificity, such as Sclerotinia sclerotiorum, a generalist capable of infecting virtually any tissue. Furthermore, a pathogen's structural specificity can change throughout its lifecycle (e.g., Figure 1g,h). Both structural specialists and generalists may have benefits considering infection strategies. Examples of such advantages include performance, competition, or niche expansion (Barrett and Heil, 2012). However, research on the processes driving structural specificity is still in its infancy and many questions are only partially

show abstract

Identification ofAvramr1fromPhytophthora infestansusing long read and cDNA pathogen-enrichment sequencing (PenSeq)

Cited by 10 publications

References 35 publications

Rpi-amr3 confers resistance to multiple Phytophthora species by recognizing a conserved RXLR effector

Rpi-amr3 confers resistance to multiple Phytophthora species by recognizing a conserved RXLR effector

Plant–Pathogen Molecular Dialogue: Evolution, Mechanisms and Agricultural Implementation

Structural specificity in plant–filamentous pathogen interactions

Contact Info

Product

Resources

About