Identification of hydrated and dehydrated lipids and protein secondary structures in seeds of cotton (Gossypium hirsutum) lines Diwas Kumar Silwal

Abstract

Cottonseeds from two parents (TM-1 and 3-79) and their 17 progeny (chromosomal substitution) lines were analyzed for various secondary structures of proteins and moisture content of lipids, separately in hulls and kernels. Fourier transform infrared spectroscopy (FTIR) was used on mature seeds from Upland cotton (G. hirsutum) progeny lines and parents. Based on secondary structures of proteins and hydration levels of lipids, differences were observed among the cottonseeds. The two progeny lines -CS-B12sh and CS-B22sh retained lipid moisture content and protein secondary structures similar to both parents, while CS-B06, CS-B15sh and CS-B16 remained distinct from either parent. On the other hand, CS-B05sh, CS-B07 and CS-B26lo were alike to TM-1 parent for lipid and protein profiles, whereas CS-B02 and CS-B04 were comparable with 3-79 parent. The capacity to detect hydrated and dehydrated lipids and different protein secondary structures using FTIR in these cottonseeds is the novel finding of this project for improving the seed nutritional traits in cotton towards cooking-oil and protein-feed usages.

Fourier Transform Infrared Spectroscopic Analysis in Applied Cotton Fiber and Cottonseed Research: A Review

“…1). Silwal et al (2017) conducted FT-IR analysis of the hull and kernels (inner meats) of delinted cottonseed from two parents (TM-1 and 3-79) and their 17 progeny (chromosomal substitution) lines. The authors assigned band areas in the region of 1700 to 1750 cm -1 for lipid, and the regions of 1665 to 1680 cm -1 , 1646 to 1660 cm -1 , 1638 to 1645 cm -1 , and 1615 to 1637 cm -1 for four secondary protein structures (respectively, β-turns, α-helices, random coils, and β-sheets) just like those of protein isolates reviewed above.…”

Chemical Composition and Thermogravimetric Behaviors of Glanded and Glandless Cottonseed Kernels

“…Cottonseed-derived oil [ 33 , 34 ], protein [ 35 , 36 ] and carbohydrate products [ 31 , 37 ] have shown different FTIR features. Specifically, FTIR intensities of 1665 to 1680 cm −1 , 1646 to 1660 cm −1 , 1638 to 1645 cm −1 , and 1615 to 1637 cm −1 have been used to evaluate cottonseed protein’s secondary structures (β-turns, α-helices, random coils, and β-sheets) [ 38 , 39 , 40 ]. Sun et al [ 41 ] used FTIR to detect the chemical and conformational changes between transgenic cottonseeds and their non-transgenic counterparts.…”