Identification of Human Remains Using Mitochondrial DNA Sequencing: Potential Mother-Child Mutational Events

Holland, Mitchell M.; Roby, Rhonda K.; Fisher, Deborah L.; Ruderman, Joan V.; Lee, D. A.; Bryson, Christine; Kupferschmid, Timothy D.; Lofts, Richard S.; Eisenberg, Arthur J.

doi:10.1007/978-3-642-78782-9_107

Cited by 4 publications

(1 citation statement)

References 19 publications

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“…The two strands of hypervariable regions HVS-I and HVS-II were amplified and sequenced following Holland and Parson [20,21]. Sequencing was performed using the BigDye terminator and primer chemistry (Supplementary Table S1).…”

Section: Control Region Sequencingmentioning

confidence: 99%

Italian mitochondrial DNA database: results of a collaborative exercise and proficiency testing

et al. 2007

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This work is a review of a collaborative exercise on mtDNA analysis undertaken by the Italian working group (Ge.F.I.). A total of 593 samples from 11 forensic genetic laboratories were subjected to hypervariable region (HVS-I/HVS-II) sequence analysis. The raw lane data were sent to MtDNA Population Database (EMPOP) for an independent evaluation. For the inclusion of data for the Italian database, quality assurance procedures were applied to the control region profiles. Only eight laboratories with a final population sample of 395 subjects passed the quality conformance test. Control region haplogroup (hg) assignments were confirmed by restriction fragment length polymorphism (RFLP) typing of the most common European hg-diagnostic sites. A total of 306 unique haplotypes derived from the combined analysis of control and coding region polymorphisms were found; the most common haplotype--CRS, 263, 309.1C, 315.1C/ not7025 AluI--was shared by 20 subjects. The majority of mtDNAs detected in the Italian population fell into the most common west Eurasian hgs: R0a (0.76%), HV (4.81%), H (38.99%), HV0 (3.55%), J (7.85%), T (13.42%), U (11.65%), K (10.13%), I (1.52%), X (2.78%), and W (1.01%).

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Section: Control Region Sequencingmentioning

confidence: 99%