Identification of human reagins isolated with immunosorbent as IgE

Kisil, F.T.; Sehon, A.H.; Yurchak, Anthony M.

doi:10.1016/0091-6749(73)90117-6

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Multiple and Sequential Elutions1

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1974

1984

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“…Similar results were obtained with the serum of another ragweed allergic patient Gre. In subsequent experiments it was demonstrated that these reaginic antibodies were associated exclusively with the IgE class of immunoglobulins [7].…”

Section: Multiple and Sequential Elutionsmentioning

confidence: 99%

Isolation of Human Reagins Differing in Their Affinity for Ragweed Immunosorbent

Kisil¹,

Attallah²,

Sehon³

et al. 1974

Int Arch Allergy Immunol

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Reaginic antibodies to ragweed pollen which had been adsorbed to cellulose-allergen immunosorbents were eluted in the presence of homologous or heterologous serum protein(s) at a concentration of 3–7 mg/ml with gly-HCl at pH 2.5, or 6 m urea at pH 7.4. These extraneous serum proteins exerted a stabilizing effect on the eluted reagins. Two groups of reagins differing in their affinity for the allergen(s) were sequentially eluted from the immunosorbent with gly-HCl and Nal regardless of the order of addition of these eluting agents. Moreover, specific elution of some reagins was achieved with a low molecular weight, hapten-like preparation of ragweed pollen.

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Section: Multiple and Sequential Elutionsmentioning

confidence: 99%