Identification of Human Bocavirus type 4 in a child asymptomatic for respiratory tract infection and acute gastroenteritis – Goiânia, Goiás, Brazil

Sousa, Teresinha Teixeira de; Almeida, Tâmera Nunes Vieira; Fiaccadori, Fabíola Souza; Souza, Menira; Badr, Kareem Rady; Cardoso, Divina das Dôres de Paula

doi:10.1016/j.bjid.2017.03.012

Cited by 8 publications

(8 citation statements)

References 19 publications

(29 reference statements)

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“…The role of Human Bocavirus as the single causative agent for respiratory tract infections remains unclear as detection in children is frequently in combination with other viruses or bacteria in a co-infection state [57,58]. Furthermore, Human Bocavirus can remain in transient state after recovery and can be isolated from healthy individuals [59]. In the last two decades, three novel coronaviruses have emerged in the humans that can cause ARTI with symptoms of fever, sore throat, cough, chills, and muscle pain leading to more severe acute respiratory distress syndrome.…”

Section: Advantages Disadvantages and Challenges In The Interpretation Of Resultsmentioning

confidence: 99%

Application of Multiplex Molecular Assays in the Diagnosis of Acute Respiratory Infectious Diseases

Amjad¹

2021

IJMB

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Acute respiratory tract infections (ARTIs) are the most common diseases worldwide and an important cause of morbidity and mortality. Upper and lower respiratory tract infections are caused by a variety of bacterial, viral, and fungal pathogens. Clinical diagnosis of respiratory tract infections is challenging because of indistinguishable symptoms. Laboratory diagnosis is performed by serology, culture, electron microscopy, and immunological antigen detection assays. These conventional diagnostic procedures are time consuming, lack sensitivity, require special laboratory setup, and well-trained staff. In the clinical laboratories, multiplex molecular nucleic acid amplification tests (NAATs) are continuously replacing conventional diagnostic methods. This review summarizes and discusses the availability, clinical use, advantages and disadvantages of multiplex molecular methods in the detection and identification of ARTI pathogens. The multiplex molecular assays can simultaneously detect 20 or more bacterial and viral pathogens and have the advantage of increased sensitivity, specificity, and rapid turn-around time. These assays are helpful in syndromic based testing in high risk patient population, particularly those who are immunocompromised, hospitalized and/or seen in the emergency department. Limitations of multiplex molecular assays include inability to detect all the possible pathogens that can be present, not being able to differentiate between asymptomatic carriers and true infections, and sometimes results are difficult to interpret. Furthermore, additional testing may be required as these assays do not provide any information regarding antimicrobial susceptibility profile. Rapid and accurate diagnosis of respiratory tract infections leads to better treatment decisions, reduction in the further diagnostic procedures, length of hospital stay, better infection control measures, and associated healthcare costs.

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Section: Advantages Disadvantages and Challenges In The Interpretation Of Resultsmentioning

confidence: 99%

Application of Multiplex Molecular Assays in the Diagnosis of Acute Respiratory Infectious Diseases

Amjad¹

2021

IJMB

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“…7,9,22 In Brazil, HBoV-4 was previously identified only once in a fecal sample from a hospitalized child with a soft tissue tumor in the submandibular region in 2015, supporting the low prevalence of HBoV-4 circulating in the country. 50 Worldwide, HBoV coinfection with other enteric viruses is reported. 11,30,32,33 Our findings indicate a higher proportion of HBoV monoinfection (65.2%) compared to coinfection (34.9%), aligning with data from Egypt and Brazil.…”

Section: Discussionmentioning

confidence: 99%

Genotypic diversity and long‐term impact of human bocavirus on diarrheal disease: Insights from historical fecal samples in Brazil

Viana,

França,

de Azevedo

et al. 2024

Journal of Medical Virology

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This study aimed to investigate the frequency and genotypic diversity of human bocavirus (HBoV) in historical fecal samples collected before 2005 in Brazil and understand its natural history in patients with diarrhea. Between 1998 and 2005, 3347 samples were tested for HBoV by RT‐PCR, with a detection rate of 5.8% (195/3347). Coinfection with norovirus (NoV) and human adenovirus (HAdV) was found in 34.9% (68/195), indicating HBoV's potential role as a causative agent of diarrheal disease. The detection rate varied over the years (p < 0.05), suggesting natural oscillatory fluctuations. HBoV was more prevalent in fall and winter, with higher positivity in children ≤5 years (p < 0.05), reinforcing that HBoV is an important pathogen in childhood diarrhea. Genotyping (32.8%; 64/195) revealed the circulation of HBoV‐1 (79.7%, 51/64), HBoV‐3 (12.5%, 8/64), HBoV‐2 (6.2%, 4/64), and the rare HBoV‐4 (1.6%, 1/64). Difference in HBoV‐1 and HBoV‐2/‐3 mono‐infections prevalence (p < 0.05), suggests a potential role of HBoV‐1 in the pathogenicity of diarrheal disease. The study highlights HBoV's lasting impact on viral gastroenteritis in Brazil and emphasizes its genotypic diversity. Recommending screening for HBoV in public health laboratories is crucial for understanding its role in gastrointestinal diseases. The data also contribute to understanding the molecular characterization of enteric viruses in historical fecal samples.

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“…The final dataset included data on children from 20 countries (Russia, United States, India, China, Brazil, Albania, Korea, Japan, Sudan, Gabon, Latvia, Iraq, Iran, South Africa, Bangladesh, Australia, Finland, United Kingdom, Thailand, and Egypt). Of the sectional in study design (20,. Amidst the cross-sectional studies, 23 were caseonly, and the remaining 2 presented data for healthy individuals.…”

Section: Study Characteristicsmentioning

confidence: 99%

“…Most recently, Neveen et al concluded that HBoV could be the significant etiologic agent of gastroenteritis due to the presence of HBoV in some children suffering from diarrheal disease without any association of any other etiological agents (19). However, a controversial issue that arises in this domain is the actual pathogen role of HBoV in gastrointestinal symptoms due to the growing appears of HBoV detection in asymptomatic patients (20,21).…”

Section: Introductionmentioning

confidence: 99%

Is Human Bocavirus Infection Associated with Gastroenteritis in Children? An Updated Systematic Review and Meta-analysis

Zhang

Liang

et al. 2021

Preprint

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Background: Human bocavirus (HBoV) figures as an increased risk factor of respiratory and gastrointestinal tract infections among children. A great deal of data is available to support the pathogenic role of HBoV in acute respiratory diseases. However, the association between HBoV infection and gastroenteritis remains controversial due to the ambiguous results. The present work aims to clarify the role of HBoV as a cause of gastroenteritis in children. Methodology/Principal findings: A systematic search of the literature was carried out from 1 January 2016 to 29 August 2021 in Embase, PubMed, Scopus, Web of Science, and the Chinese bibliographic database of biomedicine (CBM). Data from included studies were analyzed by use of a random-effects model. The pooled estimates of HBoV prevalence among all cases of gastroenteritis were generated and stratified by potential confounders. The pooled odds ratio (OR) and 95% confidence interval (CI) were computed for HBoV infection in relation to the risk of gastroenteritis. The overall prevalence of HBoV in children with gastroenteritis (9.1%, 95% CI: 6.7-11.8%) was considerably higher than that detected in children without gastroenteritis (4.0%, 95% CI: 1.1-8.5%). HBoV prevalence tended to be higher in cases of gastroenteritis under five years of age (12.1%, 95% CI: 6.8-18.7%). The highest frequency of HBoV was found in Egypt (57.8%, 95% CI: 47.7-67.6%). The predominant genotypes of HBoV circulating in children with gastroenteritis were genotype 1 (HBoV1, 3.8%, 95% CI: 2.7-5.2%) and genotype 2 (HBoV2, 2.4%, 95% CI: 1.3-3.7%). HBoV infection was significantly associated with an increased risk of gastroenteritis in children (OR 1.620, 95% CI: 1.023-2.566). Conclusion: The HBoV prevalence in pediatric cases of gastroenteritis is higher than that in children without gastroenteritis, demonstrating an increasing global burden of gastroenteritis in children caused by HBoV infection. Targeted intervention to reduce the HBoV burden should be established.

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Identification of Human Bocavirus type 4 in a child asymptomatic for respiratory tract infection and acute gastroenteritis – Goiânia, Goiás, Brazil

Cited by 8 publications

References 19 publications

Application of Multiplex Molecular Assays in the Diagnosis of Acute Respiratory Infectious Diseases

Application of Multiplex Molecular Assays in the Diagnosis of Acute Respiratory Infectious Diseases

Genotypic diversity and long‐term impact of human bocavirus on diarrheal disease: Insights from historical fecal samples in Brazil

Is Human Bocavirus Infection Associated with Gastroenteritis in Children? An Updated Systematic Review and Meta-analysis

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