Identification of Human Blood with Hybridoma-Derived Antibody to Human Immunoglobulin G

Abstract

During production of monoclonal anti-Gamma (Gm) antibody by the hybridoma technique, an antihuman immunoglobulin G (IgG) antibody was obtained. Unlike conventional antihuman IgG heteroantisera, this antibody reacted with the serum of humans and chimpanzees but did not cross-react with that of other primates or lower animal species in hemagglutination-inhibition tests with anti-D-coated red cells. To examine for the practical utility of the antihuman IgG antibody in an enzyme-linked immunosorbent assay (ELISA) for identification of human blood, microtiter wells were coated with human IgG and allowed to react with the antibody in the presence of human or animal serum under test. The bound antibody was detected with enzyme labeled antimouse IgG. The ELISA gave satisfactory results.

Forensic science

“…RIA for the platelet-specific protein /3-thromboglobulin (BTG) has been assessed for use as a sensitive marker of human blood (283). The identification of human blood with an hybridoma-derived antibody to human immunoglobulin G has been reported (284). Species identification of blood and saliva stains has been accomplished by an indirect enzymelinked immunoassay (ELISA) method using a monoclonal antibody (285).…”