“…As chilli is the non-preferred host for whiteflies, in free choice assay we cannot ensure vector infection on all the plants and in no choice essay, a single plant viruliferous whiteflies are introduced on a single plant (enclosed in a small bottle cage). Whitefly colony establishment and maintenance, to transmit chilli leaf curl viruses to test entries for screening have been successfully utilized ( Kumar et al., 2011 ; Padhi et al., 2017 ; Srivastava et al., 2017 ; Sharma et al., 2018 ; Srinivas and Thomas, 2018 ; Thakur et al., 2018 ; Barchenger et al., 2019 ; Maurya et al., 2019 ; Manisha et al., 2020 ; Yadav et al., 2020 ). Graft inoculation has been used to screen for TYLCV-resistant plants with high transmission efficiency ( Fargette et al., 1996 ).…”