Identification of HLA-A2-restricted CTL epitope encoded by the MAGE-n gene of human hepatocellular carcinoma

Dong, Hailong; Li, Zengshan; Ye, Jing; Qu, Ping; Yayu, Huang; Wu, Wen-Yih; Lu, Shao-Ying; Chen, Guangsheng; Sui, Yan-Fang

doi:10.4161/cbt.3.9.1081

Cited by 10 publications

(9 citation statements)

References 23 publications

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“…Lyophilized peptides were dissolved in DMSO and stored at -80˚C after being diluted in PBS. Total RNA was extracted from ~1x10 7 tumor cells in TRIzol reagent (Life Technologies, USA), following the recommended protocols. Briefly, total RNA was incubated at 50˚C for 30 min, to prepare the first chain of cDNA for the detection of MAGE-3 and MAGE-n.…”

Section: Methodsmentioning

confidence: 99%

“…It was highly homologous to the MAGE-A subfamily genes, especially to MAGE-3 (93%). It has been proven that the MAGE-n-derived peptide QLVFGIEVV was a new HLA-A2.1-restricted CTL epitope capable of inducing MAGE-nspecific CTLs in vitro (7). The identification of this CTL epitope opens up the possibility of clinical applications of this peptide-based cancer vaccine for patients with MAGE-n + .…”

Section: Introductionmentioning

confidence: 99%

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The anti-tumor immune response induced by a combination of MAGE-3/MAGE-n-derived peptides

Zhang

Zhang²,

Huang³

et al. 2008

Oncol Rep

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The anti-tumor immune response induced by a combination of MAGE-3/MAGE-n-derived peptides

Zhang

Zhang²,

Huang³

et al. 2008

Oncol Rep

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“…Dong et al found that MAGE-n-derived peptide QLVFGIEVV was a new HLA-A2.1-restricted CTL epitope that induces MAGE-n-specific CTLs in vitro (9). Identification of these CTL epitopes allows for clinical applications of these peptides as cancer vaccines for patients with MAGE-n + /HLA-A2 + tumors.…”

Section: Discussionmentioning

confidence: 99%

“…This study suggested that the combination of a number of tumor antigen-derived peptides is more efficacious as a peptide-based cancer immunotherapy. The combination of epitope prediction, epitope reconstruction and immunological methods improves the efficacy and accuracy of CTL epitope studies (9). The identification of human tumor-rejection antigens and CTL epitopes of these antigens allows for the development of new cancer vaccines since patients are likely to benefit from immunization with an identified CTL epitope.…”

Section: Discussionmentioning

confidence: 99%

Prediction and analysis of HLA-A2/A24-restricted cytotoxic T-lymphocyte epitopes of the tumor antigen MAGE-n using the artificial neural networks method on NetCTL1.2 Server

Zhang

Hong-yuan

et al. 2010

Oncology Letters

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Abstract.Cancer immunotherapy has become one of the most important therapeutic approaches to cancer in the past two decades. Tumor antigen-derived peptides have been widely used to elicit tumor-specific cytotoxic T lymphocytes (CTLs). Antigen-specific CTLs induced by MAGE-derived peptides have proven to be highly efficacious in the prevention and treatment of various types of tumor. MAGE-n is a new member of the MAGE gene family and has been shown to be closely associated with hepatocellular carcinoma. It is highly homologous to the MAGE-A gene subfamily, particularly to MAGE-3 (93%). MAGE-n-derived peptide QLVFGIEVV is a novel HLA-A2.1-restricted CTL epitope that induces MAGEn-specific CTLs in vitro. Identification of these CTL epitopes may lead to clinical applications of these peptides as cancer vaccines for patients with MAGE-n + /HLA-A2 + tumors. In the present study, HLA-A/A24-restricted CTL epitopes of antigen MAGE-n were predicted using the NetCTL1.2 Server on the web, COMB >0.85. The results showed that the NetCTL1.2 Server prediction method improved prediction efficacy and accuracy. Additionally, 8 HLA-A2-and 9 HLA-A24-restricted CTL epitope candidates (nonamers) derived from the tumor antigen MAGE-n were predicted. These nonamers, following identification via experimentation, may contribute to the development of potential antigen peptide tumor vaccines. IntroductionRecent studies have shown that tumor antigens, particularly tumor-specific antigens, which induce tumor-specific cytotoxic T lymphocytes (CTLs) and damage tumor cells, are a significant component of tumor vaccines. Epitope peptide vaccine has been of particular interest as it induces specific CTL in vitro and in vivo in order to kill target cells. Previously, a number of human genes that code for tumor antigens identified by autologous CTLs were isolated (1,2), and the epitopes derived from these tumor antigens were further identified to serve as targets for CTLs in the context of HLA class I molecules (3). MAGE-n is a newly identified member of the MAGE gene family which was first reported by our laboratory (Genebank, locus no. AF443295) (4), and was also found to be highly homologous to MAGE-A subfamily genes. Since HLA-A2/ A24 is one of the most frequently expressed molecules in the Chinese population (5,6), it is crucial to identify the tumor antigen epitopes which are presented by HLA-A2/A24 and to induce epitope-specific CTLs against tumor cells. The present study reported a simple and efficient bioinformatics method to identify candidate HLA-A2/A24-restricted CTL epitopes from the tumor antigen MAGE-n. Materials and methodsMAGE-n (316 aa) was selected as the antigen of interest in this study. The amino acid sequences of the tumor antigen were obtained from the Genbank database:Methods. HLA-A/A24-restricted CTL epitopes of antigen MAGE-n were predicted using the NetCTL1.2 Server on the web. The NetCTL1.2 Server (http://www.cbs.dtu.dk/services/ NetCTL) was used for CTL epitope prediction according to the following steps: i) input (past...

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“…The SacI-NotI fragment containing the EGFP coding region was isolated from pEGFP1 (Clontech, CA) and subcloned into the SacI-NotI site of pBluescriptIIKS('/) downstream of the T7 promoter to construct pBluescriptIIKS('/)-EGFP. The EcoRI-XhoI fragment encoding whole length of human MAGE-n [16] was isolated from pcDNA3.1('/)-hMAGEn and used to construct pBluescriptIIKS('/)-hMAGEn.…”

Section: Mice and Cell Linesmentioning

confidence: 99%

Immunization with truncated sequence of Telomerase Reverse Transcriptase induces a specific antitumor responsein vivo

Qiu

Sui

et al. 2007

Acta Oncologica

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Identification of HLA-A2-restricted CTL epitope encoded by the MAGE-n gene of human hepatocellular carcinoma

Cited by 10 publications

References 23 publications

The anti-tumor immune response induced by a combination of MAGE-3/MAGE-n-derived peptides

The anti-tumor immune response induced by a combination of MAGE-3/MAGE-n-derived peptides

Prediction and analysis of HLA-A2/A24-restricted cytotoxic T-lymphocyte epitopes of the tumor antigen MAGE-n using the artificial neural networks method on NetCTL1.2 Server

Immunization with truncated sequence of Telomerase Reverse Transcriptase induces a specific antitumor responsein vivo

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