Identification of Highly Specific scFvs against Total Adiponectin for Diagnostic Purposes

Abstract

Adiponectin is one of the most abundant adipokines secreted from adipose tissue. It acts as an endogenous insulin sensitizer and plasma concentrations are inversely correlated with obesity and metabolic syndrome. A decrease in plasma adiponectin levels normally indicates increased hormonal activity of the visceral lipid tissue, which is associated with decreased insulin sensitivity. It may therefore be considered a valuable biomarker for elucidating the underlying deteriorations resulting in type 2 diabetes and macrovascular disease. Here we present the use of phage display technology to identify highly specific antibody fragments (scFvs) against adiponectin. The selected scFvs showed highly specific binding to globular and native adiponectin in ELISA tests. By using our phage display technology, we were able to obtain monoclonal antibodies with specific high affinity binding to the target protein in an effective and easy to upscale manner. The selected scFvs against adiponectin can be used for developing immunoassays suitable for use in metabolic syndrome diagnosis and monitoring.

Bisphenol-A and Risk of Obesity Among A Sample of Egyptian Children: Role of Adiponectin as Biomarker of Exposure

“…Adiponectin is an insulin-sensitizing protein secreted from adipose tissue, and has been shown to improve glucose tolerance and insulin resistance in humans (Devasia et al, 2017). Many studies showed that metabolic syndrome, insulin resistance and obesity were associated with lower adiponectin (Pervanidou et al, 2013;Wilton et al, 2017). Menale et al (2016) have declared an inverse association between adiponectin and BPA urinary levels independently in obese children; they explained that exposure to BPA leads to down-regulation of adiponectin expression.…”