Identification of highly polymorphic microsatellites in the rhesus macaque <i>Macaca mulatta</i> by cross‐species amplification

Kayser, Manfred; Ritter, Heide; Bercovitch, Fred B.; Mrug, Michal; Roewer, Lutz; Nürnberg, Peter

doi:10.1111/j.1365-294x.1996.tb00302.x

Cited by 49 publications

(40 citation statements)

References 17 publications

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“…Despite of recent improvements on the procedure (for review see: Zane et al, 2002) the isolation of microsatellites is still cumbersome. One of the possible solutions for this problem is cross-species amplification, which involves the use of primer pairs designed for the flanking region of conserved microsatellites (of a so-called source species) for genotyping in related species amplification (Housley et al, 2006;Kayser et al, 1996;Kijas et al, 1995). Data for several such experiments have been reported in teleosts during the last decade (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…However, microsatellites are not always available for the species to be studied and their isolation could be time-consuming (Lin et al, 2008;Wang et al, 2008). In order to save time and effort researchers often rely on cross-species amplification Küpper et al, 2008;Kayser et al, 1996;Kijas et al, 1995;Lin et al, 2008). This procedure uses PCR primers complementary to the flanking regions of loci from a extensively studied (source) species to amplify microsatellites from closely (Harr et al, 1998) or sometimes quite distantly related species (Gonzalez-Martinez et al, 2004) for which no such markers are described.…”

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confidence: 99%

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A New Problem with Cross-Species Amplification of Microsatellites: Generation of Non-Homologous Products

Yue¹,

Kovács²,

Orbán³

2010

Zoological Research

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Abstract:Microsatellites have been widely used in studies on population genetics, ecology and evolutionary biology. However, microsatellites are not always available for the species to be studied and their isolation could be time-consuming. In order to save time and effort researchers often rely on cross-species amplification. We revealed a new problem of microsatellite cross-species amplification in addition to size homoplasy by analyzing the sequences of electromorphs from seven catfish species belonging to three different families (Clariidae, Heteropneustidae and Pimelodidae). A total of 50 different electromorphs were amplified from the seven catfish species by using primers for 4 microsatellite loci isolated from the species Clarias batrachus. Two hundred and forty PCR-products representing all 50 electromorphs were sequenced and analyzed. Primers for two loci amplified specific products from orthologous loci in all species tested, whereas primers for the other two loci produced specific and polymorphic bands from some non-orthologous loci, even in closely related non-source species. Size homoplasy within the source species was not obvious, whereas extensive size homoplasy across species were detected at three loci, but not at the fourth one. These data suggest that amplification of products from non-orthologous loci and appearance of size homoplasy by cross-amplification are locus dependent, and do not reflect phylogenetic relationship. Amplification of non-orthologous loci and appearance of size homoplasy will lead to obvious complications in phylogenetic interference, population genetic and evolutionary studies. Therefore, we propose that sequence analysis of cross-amplification products should be conducted prior to application of cross-species amplification of microsatellites.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

A New Problem with Cross-Species Amplification of Microsatellites: Generation of Non-Homologous Products

Yue¹,

Kovács²,

Orbán³

2010

Zoological Research

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“…There have been many studies carried out on the transferability of heterologous primers in various species, including Artiodactyla (Engel et al, 1996), Canidae (Gotelli et al, 1994), Mustelidae (O'Connell et al, 1996), Cetacea (Valsecchi and Amos, 1996), Pinnipedia (Coltman et al, 1996), and Primates (Kayser et al, 1996). According to a study by Barbará et al (2007), the highest percentage of amplification of polymorphic heterologous markers is observed between species of the same genus, in reptiles, birds, mammals, and invertebrates.…”

Section: Discussionmentioning

confidence: 99%

Transferability of microsatellite loci from exotic Cervidae to Brazilian brocket deer (Mazama spp, Mammalia: Cervidae)

Mantellatto¹,

Carnelossi²,

Duarte³

2010

Genet. Mol. Res.

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ABSTRACT. Transferability of microsatellite loci between closely related species has been reported in several species. This helps reduce costs involved with the development of primers for newly investigated species. Fifteen microsatellite primers developed for Rangifer tarandus, Cervus elaphus, C. axis, and Moschus berezovskii were tested on five species of Brazilian brocket deer of the genus Mazama (M. americana, M. bororo, M. gouazoubira, M. nana, and M. nemorivaga). These primers were tested with DNA extracted from blood samples of two individuals of each species obtained from the Núcleo de Pesquisa e Conservação de Cervídeos (NUPECCE) of Universidade Estadual Paulista (UNESP). Fourteen of the 15 primers tested amplified microsatellite regions of all five species of Mazama, confirmed by sequencing of the amplified fragments. We conclude that these primers could be used for population studies of brocket deer.

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“…Human polymerase chain reaction (PCR) primers have been applied to several primate species (e.g. Morin et al 1994;Gerloff et al 1995;Kayser et al 1996). Similar degrees of polymorphism are found at the equivalent primate microsatellite loci, and they can be used to discriminate between individuals.…”

Section: *Indicates Amplification Of Nontarget Product(s)mentioning

confidence: 99%

Dinucleotide microsatellite loci in a migratory wood warbler (Parulidae: Limnothlypis swainsonii) and amplification among other songbirds

1999

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Identification of highly polymorphic microsatellites in the rhesus macaque Macaca mulatta by cross‐species amplification

Cited by 49 publications

References 17 publications

A New Problem with Cross-Species Amplification of Microsatellites: Generation of Non-Homologous Products

A New Problem with Cross-Species Amplification of Microsatellites: Generation of Non-Homologous Products

Transferability of microsatellite loci from exotic Cervidae to Brazilian brocket deer (Mazama spp, Mammalia: Cervidae)

Dinucleotide microsatellite loci in a migratory wood warbler (Parulidae: Limnothlypis swainsonii) and amplification among other songbirds

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